Long-term Effects of Pyrethrin and Cyfluthrin, a Type II Synthetic Pyrethroid, Insecticide Applications on Bull Reproductive Parameters.

The objectives of this study were to determine effects of cyfluthrin and pyrethrin spray products, used in combination with cyfluthrin topical and ear tag applications, on bull reproductive parameters over 18 weeks. Angus or Angus x Simmental bulls were randomly assigned to one of three treatment groups: (i) no exposure to pyrethrins/cyfluthrin (CONT; n = 10), (ii) cyfluthrin ear tag and topical applications (ET; n = 10), or (iii) cyfluthrin ear tag, topical, premise spray and pyrethrin fog spray applications (ET+S; n = 8). Bull body weight was measured every 3 week, and body condition score and scrotal circumference were recorded on weeks 0, 9 and 18. Semen and serum were collected every 3 weeks for sperm evaluation and testosterone measurement, respectively. There was a treatment × week interaction (p < 0.01) for sperm with primary defects; bulls in CONT group had a greater (p = 0.01) percentage of sperm with primary defects than bulls treated with insecticides at week 18. Overall and progressive sperm motility, normal sperm morphology, secondary sperm defects and serum testosterone concentrations changed (p < 0.01) over time in all bulls; however, treatment did not affect (p ≥ 0.13) any of these parameters. There were also no treatment effects (p ≥ 0.08) on bull body weight, body condition score or scrotal circumference. The use of pyrethrin- and cyfluthrin-based insecticides, regardless of application, did not negatively affect reproductive parameters in beef bulls when administered over 18 weeks.

Diagnosis and effects of urine contamination in cooled-extended stallion semen.

Urospermia is known to affect semen quality in many mammals, including stallions. Determinations of semen pH and creatinine and urea concentrations have been used to diagnose urine contamination in raw stallion semen. Unfortunately, practitioners suspecting urine contamination in cooled-shipped samples have no proven means to confirm the presence of urine. Therefore, the objectives of this study were (1) to assess the effects of urine contamination on sperm motility of extended fresh and cooled-stored stallion semen, (2) to evaluate the usefulness of semen color, odor, pH, and creatinine and urea concentrations for urospermia diagnosis, and (3) to evaluate the accuracy of a commercial blood urea nitrogen test strip in diagnosing urine contamination in extended-cooled stallion semen. Thirty-seven ejaculates were obtained from 11 stallions with no history of urospermia before division into 5 mL aliquots, and contamination with stallion urine. Each resulting sample was assessed for sperm motility, color, odor, pH, creatinine, and urea nitrogen concentration using both a semiquantitative test strip (Azostix), and a quantitative automated analyzer before and after cooling for 24 hour. Sperm motility parameters, pH, and creatinine and urea concentrations were analyzed using mixed models. Urine contamination decreased total and progressive motility in all samples before and after cooling (P < 0.05). Mean control total motility was 80% at 0 hour and 67% at 24 hours, whereas urine-contaminated samples ranged from 30% to 71% at 0 hour and 27% to 61% at 24 hours. Control mean urea (29 mg/dL) and creatinine (0.6 mg/dL) concentrations were significantly different (P < 0.05) from all urine-contaminated samples (158 mg/dL and 11.6 mg/dL, respectively) at 0 hour. Similarly, control mean urea (8 mg/dL) and creatinine (0.9 mg/dL) concentrations were significantly different than all urine-contaminated samples at 24 hours. Odor assessment presented moderate sensitivity (65%) and high specificity (100%), while color assessment presented low sensitivity (47%) and moderate specificity (79%) for urine in extended semen. Azostix strips were highly sensitive (95%) and specific (97%). Assessment of color, odor, and pH are not reliable methods to diagnose urine in experimentally contaminated cooled-stored stallion semen. Sperm motility parameters (in raw and cooled semen) are significantly reduced by the presence of urine in a concentration dependent. The results of the present study indicated that determination of urea and creatinine concentrations can be used to diagnose urospermia and that Azostix can be used as a point care method for diagnosing urine contamination in extended cooled stallion semen.

Effects of local anesthesia and flunixin meglumine on the acute cortisol response, behavior, and performance of young dairy calves undergoing surgical castration.

This study assessed the effects of flunixin meglumine (FM) and a local anesthetic block (LA) on postcastration performance, plasma cortisol concentration, and behavior in dairy calves. Thirty 2- to 3-mo-old Holstein-Friesian bull calves were allocated to 5 treatments: castration with LA (2% lidocaine injected into the testes and subcutaneously), castration with FM (1.1mg/kg, i.v.), castration with LA+FM, castration without drugs (CC), and sham castration (SC). Castration was performed using a Newberry knife and Henderson castrating tool. Feed intake and body weight gain were recorded for 10d postcastration. Plasma cortisol concentration and behavior frequency and duration were monitored for 8h postcastration. Variables with repeated measures were analyzed using PROC MIXED (SAS Institute Inc., Cary, NC); one-way ANOVA was used for nonrepeated measures. No differences in feed intake or body weight gain were detected among groups. Calves in the CC, LA, and FM groups had transient (<60, <60, and <45 min, respectively) increases in plasma cortisol concentration after castration, with a second increase at 120 min in the LA group, whereas cortisol concentration remained at baseline in the LA+FM and SC groups. Mean cortisol concentrations were lower for calves in the LA+FM and SC groups than in the CC group. The area under the plasma cortisol concentration curve during the first 3h postcastration was greater in CC- and LA-treated calves than in SC controls. Castration without drugs was associated with higher frequencies of crouching and statue standing and less oral activity compared with SC controls. Administering LA alone before castration was associated with higher frequencies of head turning, statue standing, and postural changes, and less feeding behavior compared with SC controls. More leg lifting to groom was seen in LA+FM-treated calves than in SC controls. Calves administered FM alone before castration exhibited less crouching than CC calves, fewer postural shifts, and more feeding behavior than LA-treated calves. In summary, FM alone tended to shorten the duration of cortisol response and reduce crouching after surgical castration. Combining LA+FM eliminated the cortisol response to castration but was associated with more leg lifting behavior. Treatment with LA alone did not mitigate the cortisol response and was associated with several behavioral differences compared with SC, FM-treated, or FM+LA-treated calves. Results suggest that LA alone did not effectively control discomfort in young dairy calves castrated using the Henderson castration tool.

Technical note: a technique for inserting a T-cannula into the distal ileum of pregnant sows.

Our objective was to develop a technique for cannulating the terminal ileum in pregnant sows and to evaluate the usefulness of this procedure in digestibility studies in pregnant and lactating sows. A simple T-cannula was inserted into the terminal ileum approximately 15 cm cranial to the ileo-cecal valve in a total of 15 multiparous sows at d 40 (+/- 5 d) of pregnancy. All cannulated sows recovered quickly after the surgery and within 3 d they were eating normally. Elevated body temperatures were not registered in any sows, and clinical problems related to the surgery were not observed. At farrowing, normal litters were born, and number of stillborn pigs, number of live born pigs, and daily litter weight gain were not affected by the cannulations (P > .05). Of the 15 sows originally cannulated, 11 sows were used for collection of digesta during gestation and the following lactating period. Eight sows were rebred after weaning, and five sows were rebred after the second lactation period and kept for another cycle. Blockage of the cannulas never occurred, and no serious problems were associated with digesta collections. The experiment demonstrated that pregnant sows can be prepared with a simple T-cannula in the distal ileum and that the cannula can be maintained in sows throughout the reproductive cycle. Hence, the procedure provides a tool for obtaining digesta from pregnant and lactating sows for nutrition studies.

Clinical trial of moxidectin oral gel in horses.

A clinical trial carried out over 98 days was done to evaluate treatment of horses with moxidectin gel for efficacy as measured by (1) reduction in the production of parasite ova post treatment, (2) a comparison of the posttreatment parasite egg count suppression of moxidectin to ivermectin, and (3) assessment of the field safety, animal acceptance of the moxidectin formulation, and the utility of the moxidectin delivery device. One hundred and fifty Standardbred horses with naturally acquired parasite infections were used in the study. Moxidectin had more prolonged and greater suppressive influence than did ivermectin on reappearance and magnitude of strongyle egg counts post treatment. Differences were not observed between the capability of ivermectin or moxidectin to reduce and suppress low Parascaris equorum egg counts. Adverse reactions to treatments were not observed, and the utility of the moxidectin delivery syringe and animal acceptance of moxidectin treatment were satisfactory.

Plasma amino acid uptake by the mammary gland of the lactating sow.

The objectives of this study were to measure arteriovenous concentration (A-V) differences and estimate uptake of amino acids across the mammary gland in lactating sows. Four sows were used in Trial 1 and nine sows in Trial 2. Cannulas were fitted in the right anterior mammary vein and the carotid artery around d 7 of lactation. Arteriovenous samples were obtained on d 11, 14, 17, and 20. Litters were separated from the sows for 90 min, after which the first blood samples were drawn. Samples were taken at 20-min intervals for the next two consecutive hours. Milk production measurements and milk samples were obtained between d 11 and 19. Liters of plasma to liters of milk ratio (conversion coefficient) was estimated with the Fick method using A-V difference for lysine and milk lysine concentration. The conversion coefficient and the daily plasma flow were 541.41 +/- 35.72 L of plasma per liter of milk and 4,275 +/- 386 L of plasma, respectively. The A-V differences and the plasma flow were used to quantify daily amino acid uptake by the mammary gland. Uptake estimates (grams/day) were 36.51 +/- 4.05 (leucine), 31.24 +/- 3.64 (arginine), 23.39 +/- 2.97 (lysine), 21.22 +/- 1.96 (valine), 18.36 +/- 1.92 (isoleucine), 15.9 +/- 1.9 (threonine), 15.46 +/- 1.58 (phenylalanine), 7.61 +/- 1.12 (histidine), and 6.54 +/- 2.01 (methionine). The uptakes of arginine, leucine, isoleucine, valine, phenylalanine, and threonine significantly exceeded output of these amino acids in the milk. These results indicate that the sow mammary gland retains specific amino acids above requirements for milk protein synthesis.

Intrauterine diagnosis of hydrothorax in a fetus who had a combination chylothorax and pulmonary sequestration after delivery.

Congenital hydrothorax was diagnosed prenatally by transabdominal ultrasonography. Five fetal thoracentesis procedures were performed and biochemical analyses of the fluids were done. After delivery a combination chylothorax and extralobar pulmonary sequestration was demonstrated in the infant. We believe this to be the first case report of this combination investigated prenatally. We suggest that investigators perform biochemical analyses on fetal pleural fluid removed in cases with a single diagnosis. Perhaps by comparing those data with the data reported here, a biochemical marker can be identified that will be useful in distinguishing these two conditions in utero.

A technique for the venous cannulation of the mammary gland in the lactating sow.

The objective of this study was to develop a technique to cannulate the mammary venous system of the lactating sow in conjunction with the carotid artery. A total of 16 sows were subjected to surgery between d 3 and 6 of lactation. The dams were separated from their litter during the full surgical procedure and the post-surgical recovery period. The carotid artery was cannulated according to a previously described method. The mammary vein cannulation immediately followed the carotid artery cannulation. A small venous branch (approximately 4 mm in diameter) located on the lateral side of the thoracic region was used to obtain access to the mammary vein. It was isolated 4 to 5 cm above the plica lateralis, between the first and second anterior gland. The venous branch was exposed and a cannula inserted slowly for a distance of 16 cm or until the tip of the cannula would reach the most cranial point of the mammary vein. The cannula was allowed to follow the normal path of blood flow, running in a caudal to cranial direction. After recovery from surgery (1 to 2 h), the dams were returned to their respective litters and treated with antibiotics for a minimum of 6 d. All sows nursed and weaned normal litters. Cannulas were flushed with heparinized saline (20 U/mL) every 8 h and both arterial and venous blood samples (10 mL) were collected simultaneously at 0700, 1500, and 2300. Arterial and venous cannulas remained patent for a minimum of 15 d.(ABSTRACT TRUNCATED AT 250 WORDS)

Comparison of transvaginal sonography with endometrial biopsy in asymptomatic postmenopausal women.

The purpose of this study was to compare TVS with endometrial biopsy as a screening technique in asymptomatic postmenopausal women. Asymptomatic postmenopausal women were recruited by newspaper advertisement. Each study patient was subjected to pelvic examination and TVS followed by endometrial biopsy. Patients with suspected endometrial abnormalities by TVS (normal by endometrial biopsy) were evaluated further with hysteroscopy with biopsy or D&C or both. Eight patients were identified as having abnormalities by TVS, only one of whom had abnormalities by initial endometrial biopsy. Two patients were identified as having abnormalities by endometrial biopsy and normal by TVS. Further evaluation of the seven patients identified as having abnormalities by TVS (normal by endometrial biopsy) documented all seven patients as having abnormalities. The total yield of abnormalities with TVS was 16% (eight of 50 patients). The total yield of abnormalities with endometrial biopsy was 6% (three of 50 patients). The sensitivity of TVS in identifying endometrial abnormalities was 80% (eight of 10), while endometrial biopsy was only 30% (three of 10). TVS was more sensitive in detecting endometrial abnormalities, including endometrial hyperplasia, than was endometrial biopsy. The use of endometrial biopsy as a screening technique in asymptomatic postmenopausal patients is questioned.

Estimation of uterine volume with ultrasound in women with endometrial carcinoma.

In calculating uterine volume with ultrasound, it is necessary to calculate the volume of a parallelogram containing the uterus or to assume a specific shape for the uterus. The objective of this study was to determine how much (what percentage) of the parallelogram was actually occupied by the uterus without assuming any specific shape for the uterus. Overall, the volume of the uterus occupies 63% of the volume of the parallelogram. The uterus occupies a greater percentage of smaller parallelogram volumes than larger ones. The data also suggest that the concomitant presence of myomata may be responsible for the positive intercept of the regression line and therefore for the finding that small uteri occupy more than 63% of the parallelogram volume.

Chorionic villus sampling in a patient who is an unusual carrier of hemophilia B.

We describe a patient who is a carrier of hemophilia B, who was unusual in that she had symptoms and abnormal hematologic findings. She became pregnant and desired to have chorionic villus sampling for fetal sex determination. This was performed without complication. Her pregnancy continued, and she was delivered of a normal female infant with no complications for mother or infant. We believe this to be the first report of chorionic villus sampling in a symptomatic carrier of hemophilia B.

Seeking early medical attention after vaginal bleeding will not assure mild disease in postmenopausal endometrial carcinoma.

We compared the length of time between the onset of vaginal bleeding and (1) initial visit to a physician and (2) hysterectomy in patients with endometrial carcinoma (mild vs advanced disease). There was no difference in these times. We conclude that patients with advanced endometrial carcinoma do not delay seeking medical attention when compared with patients with mild disease.

Prenatal diagnosis of a placental cyst: comparison of postnatal biochemical analyses of cyst fluid, amniotic fluid, cord serum, and maternal serum.

A 4 x 4 cm placental cyst was diagnosed prenatally by ultrasonography. At delivery, simultaneous samples of cyst fluid, amniotic fluid, cord blood, and maternal blood (collected 3 hours before delivery) were obtained. Biochemical measurements of cyst fluid, amniotic fluid, cord serum, and maternal serum suggest that the solutes of cyst fluid do not originate exclusively from a single source. In addition, X-cell synthesis and secretion into the cyst fluid may occur.

Evaluation of pretreatment transvaginal ultrasonography in the management of patients with endometrial carcinoma.

OBJECTIVE: The objective of this study was to evaluate the usefulness of pretreatment assessment with ultrasonography in patients with endometrial carcinoma. STUDY DESIGN: Fifty patients with endometrial carcinoma diagnosed by endometrial biopsy or curettage were studied before hysterectomy, bilateral salpingo-oophorectomy, and selected lymph node sampling. Ultrasonographic criteria for determination of tumor grade, depth of myometrial invasion, uterine volume, and tumor volume were established. Tumor grade was compared with preoperative and postoperative pathologic diagnoses, depth of invasion was compared with postoperative pathologic evaluation, and uterine volume and tumor volume were compared with postoperative evaluation of tumor grade and depth of invasion. Data were analyzed by chi 2 testing, and, where appropriate, sensitivity, specificity, and accuracy of the ultrasonographic measurements were determined. RESULTS: The following statistically significant correlations (p less than or equal to 0.05) were found. Ultrasonography predicted tumor grade as accurately as preoperative pathologic evaluation did. Depth of invasion less than or equal to 50% or greater than 50% significantly correlated. Uterine volume less than or equal to 200 ml predicted less than or equal to 50% depth of invasion 28 of 32 times. Uterine volume greater than 500 ml was noted in six cases; five of these had grade 2 or 3 tumor and four had depth of invasion greater than 50%. Tumor volume less than or equal to 20 ml predicted a grade 1 tumor in 25 of 39 cases and depth of invasion less than or equal to 50% in 34 of 39 cases. Tumor volume greater than 20 ml correlated with a grade 2 or 3 tumor in 11 of 11 cases and a depth of invasion greater than 50% in seven of 11 cases. In 25 grade 1 tumors diagnosed postoperatively by pathologic study, none was associated with a tumor volume greater than 20 ml. CONCLUSION: Pretreatment ultrasonographic measurements should be of value in the management of patients with endometrial carcinoma.

Postcesarean endometritis: a brief review and comparison of three antibiotic regimens.

Three different antibiotic regimens (trospectomycin plus azteonam, clindamycin plus azteonam, and triple antibiotics-ampicillin plus clindamycin plus gentamicin) were all effective in treating patients with postcesarean endometritis. Patients are frequently cured clinically despite the fact that the offending organisms may be isolated in post-treatment cultures. Treatment of postcesarean endometritis without obtaining endometrial cultures is acceptable gynecologic practice. Obtaining post-treatment cultures is clearly not cost effective nor clinically beneficial. Drug treatment efficacy should be evaluated by clinical response. This communication is the first to report the new antibiotic, trospectomycin, in the treatment of postcesarean endometritis. Further clinical trials are currently underway.

Efficacy of altrenogest in synchronizing estrus in two swine breeding programs and effects on subsequent reproductive performance of sows.

In two herds that used different breeding and housing schemes, altrenogest (15 mg/d) was fed for 14 d to gilts or 10 d to sows in .45 kg of a diet formulated to meet or exceed their nutrient requirements. In Herd 1 (five breeding seasons per year), 63 of 123 gilts and 40 of 70 sows in seven replications were fed in individual crates to ensure proper intake. In Herd 2 (continuous breeding), 244 of 484 gilts in 20 replications received the treated feed in individual feeding stalls to which animals had free access. Average and median days to estrus were reduced (P less than .01) for treated gilts and sows compared with controls in both herds. Of 29 treated gilts that did not mate or become pregnant, three had cystic follicles, compared to 1 of 14 controls. There were no statistically significant treatment differences in litter size born or number of stillborn pigs in either herd, but farrowing rates of cycling gilts were 8% lower (P less than .05) in Herd 2 for treated gilts than for controls. Overall, altrenogest could be a valuable tool for improving reproductive efficiency by allowing producers to better control the estrous cycle.

Single-center comparison of results of 1000 prenatal diagnoses with chorionic villus sampling and 1000 diagnoses with amniocentesis.

Large multicenter studies have confirmed the safety and accuracy of chorionic villus sampling as a prenatal genetic diagnostic procedure, but there have been few single-center evaluations. We report our experience with 1000 consecutive chorionic villus sampling procedures compared with 1000 consecutive amniocentesis procedures during the same period. The procedures were performed by the same genetic counselors, sonographers, obstetricians, and laboratory personnel. Indications for referral, demographic characteristics of patients, numbers of attempts per patient, fetal loss rates, laboratory results, and evaluation of accuracy are included. Analysis of all data suggests that chorionic villus sampling is a safe and accurate alternative to amniocentesis in our community-based teaching hospital.

Maternal serum placental alkaline phosphatase as a marker for low birth weight: results of a pilot study.

In a pilot study, maternal serum was analyzed for placental alkaline phosphatase (PLAP) to evaluate the possibility of using PLAP values as a prenatal marker to predict low birth weight in neonates. Study subjects were selected from among women whose newborns were of low birth weight. These women were screened for maternal serum alpha-fetoprotein (MSAFP) between 15 and 20 weeks of gestation. Of the mothers of low-birth-weight neonates, 43% had PLAP values of 2.0 multiples of the median (MoM) or higher; only 22% of the mothers in the control group had such high values. The adjusted odds ratio was 2.6 (95% confidence interval, 1.1 to 6.5) and was not confounded by race, age, or weight. Odds ratios were improved by selecting more extreme cutoff values, with fewer cases identified as positive. In 14% of the cases of low birth weight, both MSAFP and PLAP values were elevated, compared with 5.6% of those in the control group. These data suggest that PLAP elevations, with or without measurements for MSAFP, may be a useful indicator during the second trimester of pregnancy for the risk of low birth weight.

The effects of articulation on the acoustical structure of feline vocalizations.

Feline isolation calls were analyzed, and a model was developed to relate the acoustical features of these calls to the physical processes used in their production. Fifty isolation calls were recorded from each of five cats for a total sample of 250 vocalizations. By combinations of Fourier transform, autocorrelation, and linear prediction methods, the fundamental frequency (glottal-pulse period) F0, the energy of F0, the frequency having maximum energy Fmax (not always F0), and the energy at this frequency were computed. Mean F0 ranged from 400-600 Hz for individual cats. For some cats F0 was consistent within calls, but for other cats sudden shifts in F0 occurred within calls. Here, Fmax was almost a harmonic of F0 and generally ranged from 1-2 kHz. For individual cats, the energy ratio E = (energy of Fmax/energy of F0) varied from 1 to 60 and the grand average E over the time course of the call varied from about 12 to 38. The mean rms call intensity was an inverted-U function of time. Measured jaw opening was strongly correlated with acoustical features of call. A Bessel-horn model with time-varying flare gave a good account of acoustical parameters such as Fmax. The presence of formantlike resonances in cat vocalizations and the important role of jaw movements (vocal gestures) in the production of these calls suggest that cats may provide a useful model for some aspects of human vocal behavior.