Telomere length determined by the fluorescence in situ hybridisation distinguishes malignant and benign cells in cytological specimens.

BACKGROUND: Telomeres are tandem repeats of TTAGGG at the end of eukaryotic chromosomes that play a key role in preventing chromosomal instability. The aim of the present study is to determine telomere length using fluorescence in situ hybridisation (FISH) on cytological specimens. METHODS: Aspiration samples (n = 41) were smeared on glass slides and used for FISH. RESULTS: Telomere signal intensity was significantly lower in positive cases (cases with malignancy, n = 25) as compared to negative cases (cases without malignancy, n = 16), and the same was observed for centromere intensity. The difference in DAPI intensity was not statistically significant. The ratio of telomere to centromere intensity did not show a significant difference between positive and negative cases. There was no statistical difference in the signal intensities of aspiration samples from ascites or pleural effusion (n = 23) and endoscopic ultrasound-guided FNA samples from the pancreas (n = 18). CONCLUSIONS: The present study revealed that telomere length can be used as an indicator to distinguish malignant and benign cells in cytological specimens. This novel approach may help improve diagnosis for cancer patients.

Natural skeletal levels of lead in Homo sapiens sapiens uncontaminated by technological lead.

Lead, Ba and Ca concentrations were determined in tooth enamel, femur and rib from buried skeletons of PreColumbian Southwest American Indians, 10 subjects who lived 1000 years ago on the Pacific coast at 34 degrees N, and 13 subjects who lived 700 years ago in a desert valley tributary of the Colorado River at 37 degrees N 111 degrees W, both groups living in environments uncontaminated by technological Pb. For the coastal tribe, average Pb/Ca ratios were 1.1 x 10(-7) in enamel, 2.3 x 10(-7) in femur and 4.7 x 10(-7) in rib, while Ba/Ca ratios were 1.2 x 10(-5) in enamel, 32 x 10(-5) in femur and 38 x 10(-5) in rib (wt ratios). For the desert tribe, average Pb/Ca ratios were 4 x 10(-7) in enamel, 11 x 10(-7) in femur and 37 x 10(-7) in rib, while Ba/Ca ratios were 1.1 x 10(-5) in enamel, 7.5 x 10(-5) in femur and 6.2 x 10(-5) in rib. It is shown that biologic levels of Pb and Ba in buried femur and rib at both burial sites and in buried enamel at the Arizona site are obscured by excessive diagenetic additions of Pb and Ba from soil moisture. It is shown that one-third of the Pb in enamel at the Malibu site is biologic, yielding a skeletal Pb/Ca (wt) ratio of 4 x 10(-8). This is equivalent to a mean skeletal concentration of 13 ng Pb g-1 bone ash, and a mean natural body burden of 40 micrograms Pb/70 kg adult Homo sapiens sapiens, uncontaminated by technological Pb. This value is about one-thousandth of the mean body burden of 40 mg industrial Pb/70 kg adult American today, which indicates the probable existence within most Americans of dysfunctions caused by poisoning from chronic, excessive overexposures to industrial Pb.

Lead in ancient human bones and its relevance to historical developments of social problems with lead.

Concentrations of metabolic lead in buried ancient bones are obscured by replacement of calcium in apatite by excessive amounts of soil moisture Pb. Concentrations of metabolic barium in bones are affected in a similar way. Added soil Pb and Ba, expressed as log(Pb/Ca) versus log(Ba/Ca) among various bones at a given burial site, are positively covariant, with about 5-fold more soil Pb added for each unit of added soil Ba. The typical natural metabolic Ba/Ca ratio in contemporary people can be measured unambiguously because it as unaffected by industrial pollution. It applies to ancient people because it has not changed historically. The intercept of the covariance curve for buried bones of a given ancient population at the known metabolic Ba/Ca ratio indexes the corresponding metabolic Pb/Ca ratio in bones of that population. Lead levels which prevailed in Romans appear to have been similar to those in contemporary people, which are approximately 1000-fold above natural levels in humans determined by this method in ancient Peruvians. This indicates that studies of natural biochemical reactions in cells free of industrial Pb should be made, because most present biochemical knowledge is founded on data obtained from systems polluted with Pb 1000 to 100000-fold above natural levels. The 5000 year history of smelting Pb by humans indicates that a system of education fostered by genetically common lower brain center functions operated on hundreds of successive generations in a context of cultural changes invoked by feedback from developments in engineering technologies to give rise to the difference between present typical and prehistoric natural levels of Pb in humans. Archaeological and anthropological studies of early developments in writing, music and metallurgy by ancient Peruvians and Persian peoples should be combined with PET-scan studies of their descendants to discover if, as preliminary archaeological data suggest, the two ancient populations differed on a genetic basis in higher brain functions, yet are indistinguishable as metallurgical engineers. This would demonstrate that higher centers of the human brain did not exercise guiding control, through hundreds of generations, over those developments of engineering technologies which resulted in the extreme pollution of the earth's biosphere with poisonous Pb.

Skeletal concentrations of lead in ancient Peruvians.

The level of biologic lead (expressed as the ratio of atomic lead to atomic calcium) in bones of Peruvians buried 1600 years ago was found to be 3 x 10(-8), as compared to 2100 to 3500 x 10(-8) in the bones of present-day residents of England and the United States. The ratio of barium to calcium was 2 to 3 x 10(-6) in bones of ancient Peruvians and present-day Americans. Barium and lead have similar morphologic distributions in organisms, so this discrepancy for lead must result from overexposure of present-day people to industrial lead and not from natural variations. The magnitude of this discrepancy has been confirmed by two different lines of investigation not reported in this article. This new evidence suggests that natural interactions of lead in human cells have not yet been determined because reagents, nutrients and controls used in laboratory and field studies have been contaminated with lead far in excess of naturally occurring levels.