RESUMO
A simple membrane dialysis bioreactor was developed for a large-scale axenic culture of Symbiobacterium thermophilum, a symbiotic thermophile that requires co-cultivation with an associating thermophilic Bacillus strain S for normal growth. The bioreactor consisted of an outer- and an inner-coaxial cylindrical compartment bordered across a dialyzing membrane, which enabled a 1 l-scale dialysis culture with exchange of low molecular metabolites between the two compartments to be performed. Using the bioreactor, growth characteristics of S. thermophilum and Bacillus strain S were assessed under two medium conditions. The growth of S. thermophilum was measured by quantitative PCR because the bacterium formed no visible colonies and gave abnormally low turbidity. In medium containing 2% tryptone peptone, S. thermophilum proliferated up to 4x10(7) cells/ml, and strict dependence on the co-culture with Bacillus strain S was observed. On the other hand, medium containing 0.5% yeast extract not only facilitated the growth of S. thermophilum in the co-culture (6x10(7) cells/ml), but also allowed limited pure growth independent of Bacillus strain S (1x10(7) cells/ml), implying that some component of yeast extract can partially replace the growth requirement of S. thermophilum supplied by Bacillus strain S. Both the oxidative redox potential values and the cell morphology in the independently growing culture suggested the occurrence of marked unbalanced growth possibly caused by significant metabolic changes. The bioreactor is applicable to the analyses of culturing characteristics in symbiotic systems between free-living microorganisms.
Assuntos
Reatores Biológicos , Diálise/instrumentação , Bactérias Gram-Positivas/crescimento & desenvolvimento , Simbiose/fisiologia , Meios de CulturaRESUMO
Left-right asymmetric signaling molecules in mammals include three transforming growth factor beta (TGFbeta)-related factors, Nodal, Lefty1 and Lefty2. They are all expressed on the left half of developing mouse embryos. Nodal acts as a left-side determinant by transducing signals through Smad and FAST and by inducing Pitx2 expression on the left side. Lefty proteins are antagonists that inhibit Nodal signaling. There are positive and negative transcriptional regulatory loops between nodal and lefty2 genes. Thus, Nodal activates its own gene and lefty2. Lefty2 protein produced then inhibits Nodal signaling and terminates expression of both genes. This feedback mechanism can restrict the range and duration of Nodal signaling in developing embryos.
Assuntos
Padronização Corporal/genética , Anormalidades Congênitas/genética , Animais , Anormalidades Congênitas/fisiopatologia , Embrião de Mamíferos/metabolismo , Feminino , Regulação da Expressão Gênica no Desenvolvimento , Fatores de Determinação Direita-Esquerda , Masculino , Camundongos , Camundongos Endogâmicos C3H , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos , Camundongos Transgênicos , Proteína Nodal , Transdução de Sinais , Fator de Crescimento Transformador beta/genéticaRESUMO
The effect of CD44-phenotypic expression on metastasis to the lung was studied using a spontaneous murine osteosarcoma-derived cell line, POS-1, stimulated with lipopolysaccharide (LPS). POS-1 cells were inoculated into the hind paws of 20 C3H/HeJ mice and produced a visible mass in all mice in 5 weeks, and these transplanted tumors resulted in lung metastasis in all mice. The number of metastatic foci in the lungs was 12.0+/-2.1 (mean+/-SD) with LPS-stimulated cells, which was significantly higher than that of unstimulated cells (5.8+/-1.4; N=10 for each; P<0.05). Hyaluronate (HA), a ligand of CD44, inhibited a number of lung metastases in a dose-dependent manner (0.5% HA, 3.0+/-1.1; 0.005% HA, 5.1+/-1.5; without HA, 8.6+/-1.7; N=10 for each; P<0.05, each group with HA versus the group without HA). Adhesion assay by coculturing POS-1 cells and lung microvascular endothelial cells on culture plate showed that the adhesion was significantly lower in HA treated POS-1 than those without HA (1.18+/-0.12 and 2.74+/-0.17, respectively, P<0.05). These results suggest that lung metastasis was accelerated by up-regulation of CD44.
Assuntos
Receptores de Hialuronatos/metabolismo , Neoplasias Pulmonares/secundário , Osteossarcoma/secundário , Animais , Adesão Celular , Modelos Animais de Doenças , Endotélio Vascular/fisiologia , Lipopolissacarídeos , Neoplasias Pulmonares/metabolismo , Camundongos , Camundongos Endogâmicos C3H , Invasividade Neoplásica , Transplante de Neoplasias , Osteossarcoma/metabolismo , Osteossarcoma/patologia , Células Tumorais Cultivadas , Regulação para Cima/efeitos dos fármacosRESUMO
FoxH1 (FAST) is a transcription factor that mediates signaling by transforming growth factor-beta, Activin, and Nodal. The role of FoxH1 in development has now been investigated by the generation and analysis of FoxH1-deficient (FoxH1(-/-)) mice. The FoxH1(-/-) embryos showed various patterning defects that recapitulate most of the defects induced by the loss of Nodal signaling. A substantial proportion of FoxH1(-/-) embryos failed to orient the anterior-posterior (A-P) axis correctly, as do mice lacking Cripto, a coreceptor for Nodal. In less severely affected FoxH1(-/-) embryos, A-P polarity was established, but the primitive streak failed to elongate, resulting in the lack of a definitive node and its derivatives. Heterozygosity for nodal renders the FoxH1(-/-) phenotype more severe, indicative of a genetic interaction between FoxH1 and nodal. The expression of FoxH1 in the primitive endoderm rescued the A-P patterning defects, but not the midline defects, of FoxH1(-/-) mice. These results indicate that a Nodal-FoxH1 signaling pathway plays a central role in A-P patterning and node formation in the mouse.
Assuntos
Padronização Corporal , Proteínas de Ligação a DNA/genética , Fator de Crescimento Epidérmico , Glicoproteínas de Membrana , Transdução de Sinais , Fatores de Transcrição/genética , Fator de Crescimento Transformador beta/genética , Animais , Proteínas de Ligação a DNA/metabolismo , Desenvolvimento Embrionário e Fetal , Fatores de Transcrição Forkhead , Regulação da Expressão Gênica no Desenvolvimento , Camundongos , Camundongos Knockout , Mutação , Proteínas de Neoplasias/genética , Proteína Nodal , Fenótipo , Reação em Cadeia da Polimerase , Fatores de Transcrição/metabolismo , Fator de Crescimento Transformador beta/metabolismoRESUMO
Pitx2 is left--right (L--R) asymmetrically expressed initially in the lateral plate and later in primordial visceral organs. The transcriptional regulatory mechanisms that underlie L--R asymmetric expression of Pitx2 were investigated. Mouse Pitx2 has a left side-specific enhancer (ASE) that mediates both the initiation and maintenance of L--R asymmetric expression. This element contains three binding sites for the transcription factor FAST. The FAST binding sites function as Nodal-responsive elements and are sufficient for the initiation but not for the maintenance of asymmetric expression. The maintenance requires an Nkx2-5 binding site also present within the ASE. These results suggest that the left-sided expression of Pitx2 is directly initiated by Nodal signaling and is subsequently maintained by Nkx2. Such two-step control may represent a general mechanism for gene regulation during development.
Assuntos
Regulação da Expressão Gênica no Desenvolvimento , Proteínas de Homeodomínio/genética , Proteínas Nucleares , Fatores de Transcrição/genética , Fator de Crescimento Transformador beta/genética , Proteínas de Xenopus , Animais , Sequência de Bases , Sítios de Ligação/fisiologia , Padronização Corporal/genética , Proteínas de Ligação a DNA/química , Proteínas de Ligação a DNA/genética , Proteínas de Ligação a DNA/metabolismo , Elementos Facilitadores Genéticos/fisiologia , Fatores de Transcrição Forkhead , Proteína Homeobox Nkx-2.5 , Proteínas de Homeodomínio/química , Proteínas de Homeodomínio/metabolismo , Óperon Lac , Fatores de Determinação Direita-Esquerda , Camundongos , Dados de Sequência Molecular , Proteína Nodal , Fatores de Transcrição Box Pareados , Transdução de Sinais/genética , Fatores de Transcrição/química , Fatores de Transcrição/metabolismo , Transgenes/fisiologia , Xenopus , Proteína Homeobox PITX2RESUMO
Retinoic acid (RA), a derivative of vitamin A, plays a pivotal role in vertebrate development. The level of RA may be determined by the balance between its synthesis and degradation. We have examined the role of CYP26, a P450 enzyme that may degrade RA, by generating mutant mice that lack CYP26. CYP26(-/-) mice exhibited anomalies, including caudal agenesis, similar to those induced by administration of excess RA. The concentration of endogenous RA, as revealed by marker gene activity, was markedly increased in the tailbud of the mutant animals, in which CYP26 is normally expressed. Expression of T (Brachyury) and Wnt3a in the tailbud was down-regulated in CYP26(-/-) mice, which may underlie the caudal truncation. The lack of CYP26 also resulted in homeotic transformation of vertebrae as well as in misspecification of the rostral hindbrain associated with anterior expansion of RA-positive domains. These results suggest that local degradation of RA by CYP26 is required for establishing an uneven distribution of RA along the anterio-posterior axis, which is essential for patterning the hindbrain, vertebrae, and tailbud.
Assuntos
Sistema Enzimático do Citocromo P-450/metabolismo , Embrião de Mamíferos/metabolismo , Proteínas Fetais , Tretinoína/metabolismo , Animais , Sequência de Bases , Padronização Corporal , Sistema Enzimático do Citocromo P-450/deficiência , Sistema Enzimático do Citocromo P-450/genética , Primers do DNA/genética , Regulação para Baixo , Membro Anterior/embriologia , Regulação da Expressão Gênica no Desenvolvimento , Camundongos , Camundongos Knockout , Camundongos Mutantes , Camundongos Transgênicos , Proteínas/genética , Proteínas/metabolismo , Ácido Retinoico 4 Hidroxilase , Rombencéfalo/embriologia , Somitos/metabolismo , Proteínas com Domínio T/genética , Proteínas com Domínio T/metabolismo , Distribuição Tecidual , Tretinoína/antagonistas & inibidores , Proteínas Wnt , Proteína Wnt3 , Proteína Wnt3ARESUMO
A Gram-negative and tryptophanase-positive thermophile, whose growth is dependent on co-culture with an associating Bacillus strain, had been reported and tentatively named Symbiobacterium thermophilum strain T(T). Axenic culture of strain T(T) was recently established by dialysing cultures with the supporting bacterial strains or adding their culture broth. Phylogenetic analysis of strain T(T), based on the 16S rDNA sequence, was conducted for the validation of S. thermophilum. The sequence of strain T(T) was located at the outermost position in the high-G+C Gram-positive group distinctly isolated from any other branches hitherto known. Ten sequences identical to that of strain T(T), and one sequence closely related to it, were identified for the first time from soil and compost samples. The outer membrane of strain T(T) had a three-layered structure, outside the cytoplasmic membrane, which is similar to the S-layer in the cells of members of the Bacillaceae. Chemical analysis of the cells revealed that menaquinone-6 is a major component of the quinone system. According to these results, along with several previous observations (i.e. a G+C DNA content of 65 mol% and the identification of iso-C15:0 and iso-C17:0 acids as major cellular fatty acids), the new taxon Symbiobacterium thermophilum gen. nov., sp. nov. is proposed. The type strain is S. thermophilum strain T(T) (= IAM 14863T).
Assuntos
Bacillus/crescimento & desenvolvimento , Bactérias Gram-Negativas/classificação , Bactérias Gram-Negativas/crescimento & desenvolvimento , Bacilos Gram-Positivos Asporogênicos/classificação , Bacilos Gram-Positivos Asporogênicos/crescimento & desenvolvimento , Microbiologia do Solo , Simbiose , Composição de Bases , Meios de Cultura , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Bactérias Gram-Negativas/genética , Bactérias Gram-Negativas/ultraestrutura , Bacilos Gram-Positivos Asporogênicos/genética , Bacilos Gram-Positivos Asporogênicos/ultraestrutura , Temperatura Alta , Dados de Sequência Molecular , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Temperatura , Triptofanase/metabolismoRESUMO
Three sets of dyads, in which a zinc-porphyrin (ZP) electron donor is connected to an aromatic diimide electron acceptor,either pyromellitimide (PI) or naphthalene-1,8:4,5-tetracarboxylic acid diimide (NI), via a boronate-ester bridge, a piperidine bridge, and a 1,3-dioxolane bridge, respectively, were prepared for the purpose of control of intramolecular electron transfer (ET) by acid-base reactions at the connecting bridge. Boronate-ester bridge is a Lewis acidic site and confers a chance to regulate intramolecular ET reaction upon base coordination. This has been demonstrated by suppression of photoinduced ET from ZP to PI or NI in highly electron-pair donating solvents or upon addition of a fluoride anion. To extend this strategy to control of ET-path selectivity, we prepared triad 18, which consists of a ZP donor bearing NI and PI acceptors at similar distances through a boronate-ester bridge and an acetal bridge, respectively. Photoexcitation of 18 in a free form led to intramolecular ET from (1)()ZP preferentially to NI, but the ET path was completely switched toward PI in F(-)-coordinated form without a serious drop in the rate, constituting a novel ET-switching molecular system.
RESUMO
We reported successful anesthetic management of a 34 year-old woman with diffuse pulmonary hamartoangiomyomatosis associated with spontaneous pneumothorax. She had recurrent pneumothorax and chest X-ray film showed reticulo-granular shadow, and chest CT revealed multiple bullae. Induced by thiopental and succinylcholine, anesthesia was maintained safely with enflurane in oxygen. Nitrous oxide was used after thoracotomy. This disease accompanies multiple bullae in the lung and some patients have complications such as tuberous sclerosis or hemangioma of the kidney. Therefore, we have to pay attention to both bullae and other complications.
Assuntos
Anestesia por Inalação , Neoplasias Pulmonares/complicações , Linfangiomioma/complicações , Pneumotórax/complicações , Adulto , Enflurano , Feminino , Humanos , Neoplasias Pulmonares/cirurgia , Linfangiomioma/cirurgia , Óxido Nitroso , Oxigênio , Pneumotórax/cirurgiaRESUMO
STI measurements and impedance cardiography were carried out during gestation and post partum in 231 cases. In normal pregnant women, prolongation of the Q-I interval, isometric contraction time (ICT), preejection period (PEP) and shortening both of the Q-II interval and ejection time (ET) were all marked in late stages of pregnancy. Mean values for stroke volume (SV), cardiac output (CO) and cardiac index (CI) were at the maximum at 16-23 weeks of pregnancy, and then fell in the antepartum period. The total peripheral resistance (TPR) at 16-23 weeks of pregnancy was lower than in other periods. In the postpartum period, the heart rate was lowered, and the Q-II interval and ICT were prolonged. In toxemic pregnancy, SV, CO and CI were lower than those of the normal group and various changes in STI were more apparent than those of non-toxemic pregnants in the supine position. By the postural change from supine to left lateral in toxemia, the improvement in STI was not as good as in the normal pregnant group and, moreover, SV and CO were decreased and TPR was increased. These results suggest that in toxemic pregnancy the left ventricle might be less complaint and less sensitive to the increase in venous return.