RESUMO
HIV risk perceptions and behaviors of 236 commercial sex workers from three major Mozambican urban centers were studied using the International Rapid Assessment, Response and Evaluation (I-RARE) methodology. All were offered HIV testing and, in Maputo, syphilis testing was offered as well. Sixty-three of the 236 opted for HIV testing, with 30 (48%) testing positive for HIV. In Maputo, all 30 receiving HIV tests also had syphilis testing, with 6 (20%) found to be positive. Results include interview excerpts and qualitative results using I-RARE methodology and AnSWR-assisted analyses of the interviews and focus group sessions.
Assuntos
Cidades/estatística & dados numéricos , Infecções por HIV , Conhecimentos, Atitudes e Prática em Saúde , Profissionais do Sexo/psicologia , Comportamento Sexual/psicologia , Pessoal Administrativo , Adolescente , Adulto , Aconselhamento , Usuários de Drogas/psicologia , Usuários de Drogas/estatística & dados numéricos , Infecções por HIV/diagnóstico , Infecções por HIV/etiologia , Pessoal de Saúde , Humanos , Masculino , Moçambique , Risco , Adulto JovemAssuntos
Linfócitos T CD4-Positivos/imunologia , Células Dendríticas/imunologia , Suscetibilidade a Doenças/imunologia , Doença de Hodgkin/imunologia , Infecções Oportunistas/imunologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Contagem de Células Sanguíneas , Suscetibilidade a Doenças/sangue , Feminino , Doença de Hodgkin/sangue , Humanos , Interferon-alfa/sangue , Interferon-alfa/imunologia , Masculino , Pessoa de Meia-Idade , Infecções Oportunistas/sangue , Adulto JovemRESUMO
Myeloid and plasmacytoid dendritic cells (DCs) are important mediators of both innate and adaptive immunity against pathogens such as HIV. During the course of HIV infection, blood DC numbers fall substantially. In the present study, we sought to determine how early in HIV infection the reduction occurs and whether the remaining DC subsets maintain functional capacity. We find that both myeloid DC and plasmacytoid DC levels decline very early during acute HIV infection. Despite the initial reduction in numbers, those DCs that remain in circulation retain their function and are able to stimulate allogeneic T-cell responses, and up-regulate maturation markers plus produce cytokines/chemokines in response to stimulation with TLR7/8 agonists. Notably, DCs from HIV-infected subjects produced significantly higher levels of cytokines/chemokines in response to stimulation with TLR7/8 agonists than DCs from uninfected controls. Further examination of gene expression profiles indicated in vivo activation, either directly or indirectly, of DCs during HIV infection. Taken together, our data demonstrate that despite the reduction in circulating DC numbers, those that remain in the blood display hyperfunctionality and implicates a possible role for DCs in promoting chronic immune activation.
Assuntos
Células Dendríticas/imunologia , Infecções por HIV/imunologia , Contagem de Células Sanguíneas , Linfócitos T CD4-Positivos/imunologia , Linfócitos T CD8-Positivos/imunologia , Estudos de Casos e Controles , Quimiocinas/biossíntese , Quimiocinas/genética , Estudos Transversais , Citocinas/biossíntese , Citocinas/genética , Células Dendríticas/classificação , Expressão Gênica , Infecções por HIV/sangue , Infecções por HIV/genética , Infecções por HIV/virologia , Humanos , Técnicas In Vitro , Estudos Longitudinais , Ativação Linfocitária , Transdução de Sinais , Fatores de Tempo , Receptores Toll-Like/agonistas , Carga ViralRESUMO
Antigen presenting dendritic cells (DCs) can serve as sites for HIV replication and as vehicles for transmission of the virus to T cells. It is known that the numbers of DCs in blood is reduced during HIV-1 infection. Here we monitored the two major subsets of blood DCs in 12 individuals undergoing a change, primarily initiation, of highly active antiretroviral therapy. The numbers of plasmacytoid DCs were reliably higher on therapy, although in the 1-3 month interval we followed, these numbers did not return to those seen in HIV uninfected controls. An increase in plasmacytoid DCs was accompanied by an increase in IFN-alpha production in response to a standard challenge in culture with UV-inactivated herpes simplex virus. The levels of myeloid DCs also demonstrated an increase while on HAART, and these numbers become comparable to the HIV uninfected controls. The numbers of plasmacytoid and myeloid DCs varied inversely with the levels of plasma HIV viremia. These longitudinal studies extend prior work showing that virus infection with HIV leads to a decrease in the number of dendritic cells in blood, and that this can be reversed at least in part by therapy.
Assuntos
Antirretrovirais/farmacologia , Células Dendríticas/efeitos dos fármacos , Células Dendríticas/virologia , Adulto , Antirretrovirais/uso terapêutico , Contagem de Células Sanguíneas , Linfócitos T CD4-Positivos/efeitos dos fármacos , Linfócitos T CD4-Positivos/patologia , Estudos de Casos e Controles , Células Dendríticas/patologia , Feminino , Infecções por HIV/sangue , Infecções por HIV/tratamento farmacológico , Infecções por HIV/imunologia , Humanos , Interferon-alfa/biossíntese , Leucócitos Mononucleares/efeitos dos fármacos , Leucócitos Mononucleares/patologia , Estudos Longitudinais , Masculino , Pessoa de Meia-Idade , Carga ViralRESUMO
The host immune factors that determine susceptibility to HIV-1 infection are poorly understood. We compared multiple immunologic parameters in three groups of HIV-1-seronegative men: 14 highly exposed (HR10), 7 previously reported possibly to have sustained transient infection (PTI), and a control group of 14 low risk blood bank donors (BB). Virus-specific cellular immune assays were performed for CD4(+) T helper cell responses, CD8(+) cytotoxic T lymphocyte activity, CD8(+) cell chemokine release, and CD8(+) cell-derived antiviral soluble factor activity. General immune parameters evaluated included CCR5 genotype and phenotype, interferon alpha production by PBMCs, leukocyte subset analysis, and detailed T lymphocyte phenotyping. Comparisons revealed no detectable group-specific differences in measures of virus-specific immunity. However, the HR10 group differed from the BB group in several general immune parameters, having higher absolute monocyte counts, higher absolute CD8(+) T cell counts and percentages, lower naive and higher terminal effector CD8(+) cells, and lower levels of CD28(+)CD8(+) cells. These changes were not associated with seropositivity for other chronic viral infections. The PTI men appeared to have normal levels of monocytes and slightly elevated levels of CD8(+) T cells (also with increased effector and decreased naive cells). Although we cannot entirely exclude the contribution of other chronic viral infections, these findings suggest that long-lived systemic cellular antiviral immunity as detected by our assays is not a common mechanism for resistance to infection, and that resistance may be multifactorial. General immune parameters reflected by CD8(+) T cell levels and activation, and monocyte concentrations may affect the risk of infection with HIV-1, and/or serve as markers of exposure.