Changes in oxidative stress intensity in blood of tumor-bearing rats following different modes of administration of rhenium-platinum system.

Effects of the different modes of administration of dichlorotetra-µ-isobutyratodirhenium(ІІІ) ­ І ­ (in water solution, liposomes, nanoliposomes and together with cisplatin ­ in the rhenium-platinum system) on the intensity of lipid peroxidation (LP) in blood plasma and the activity of the erythrocyte antioxidant enzymes were investigated on the model of tumor growth. A decrease in the concentration of TBA-active substances caused by dirhenium compounds was shown to be independent of the administration mode and the extent of the tumor growth inhibition. I was four-times more effective in inhibition of the LP burst than any known antioxidant. I induced the increasing activity of erythrocyte superoxide dismutase and decreasing activity of catalase. In vitro experiments with native superoxide dismutase, the interaction of І with following activation of the active center of the enzyme was confirmed and the superoxide dismutase activity of І was shown, that may contribute to the enhancement of the enzyme activity in vivo. The cluster rhenium compounds may be promising nontoxic potent antioxidants capable of deactivating superoxide radicals.

[Antioxidant properties of cluster rhenium compounds and their effect on erythropoiesis of rats with guerin carcinoma].

Biochemical characteristics of kidneys, pe- ripheral blood and bone marrow of rats in model of tumor growth under introduction of cisplatin and cis-tetrachlorodi-µ-isobutyratodirhenium(III), cis-Re2(i-C3H7COO)2Cl4 (I) have been investigated. It was shown that introduction of I alone and together with cisplatin led to decrease of biochemical markers of oxidation of lipids and proteins in tissue homogenates of the kidneys, change of enzyme activity in the urea and tissue homogenates of the kidneys, by a decrease of filtration function of kidneys. Introduction of nanoliposomal forms of the rhenium cluster compound led to a practically normal morphological picture of bone marrow and increase of the RBC (by 60%) with normalization of hematocrit counts, and decrease of quantities of destructed RBC (3.2 times) in comparison with the tumor-bearing animals. A tentative scheme of influence of cluster rhenium compound on erythropoiesis through regulation of synthesis of erythropoietin in kidneys has been proposed.

[Antioxidant and antitumor activity of dirhenium dicarboxylates in animals with guerin carcinoma].

The antioxidant and anticancer properties of dirhenium dicarboxylates of cis- and transconfiguration with different organic ligands in a model of tumor growth (Guerin carcinoma) were studied. It was shown that compounds of different configuration had similar antitumor effect, and dirhenium (III) cis-dicarboxylates were characterized by higher antioxidant activity and degree of activation of erythrocyte superoxide dismutase (SOD) in comparison with trans-isomers. The dependence between the structure of dirhenium (III) dicarboxylates and their ability to activate erythrocyte SOD in the model of tumor growth was shown for the first time. The in vitro studies have shown that rhenium compounds of cis- and transconfiguration interacted similarly with erythrocyte SOD, changing the protein secondary structure. In contrast to trans-dicarboxylate, for cis-dicarboxylate the SOD-like activity was demonstrated to be on the first minutes of the xantine-oxidase reaction. The studied features of the interaction between rhenium compounds and SOD in vitro explain only partly the activation of SOD in experiments in vivo. The attempt is made to explain the differences in the mechanisms of antioxidant activity of dirhenium cis- and trans-dicarboxylates.

[Changes of the state of rat kidneys under guerin carcinoma development and use of cytostatics].

It was shown that development of the Guerin carcinoma and introduction of cisplatin led to the damage of the kidneys of rats that was confirmed by a relative increase of weight, proteinuria, change of gamma-glutamyl transpeptidase and lactate dehydrogenase activity in the urea and tissue homogenates of the kidneys, by a decrease of relative reabsorption and glomerular filtration. Introduction of nanoliposomal forms of the rhenium cluster compounds led to normalization of above mentioned diagnostic indexes and to reduction of the toxic cisplatin influence that was confirmed by biochemical and morphological investigations.

[Influence of antitumor system rhenium-platinum on biochemical state of the liver].

Influence of the antitumour rhenium-platinum system on biochemical liver characteristics in the model of tumor growth (Guerin carcinoma) was studied and possible hepatoprotective activity of rhenium cluster compounds when introducing them in different forms was shown, that was confirmed by decreasing of diagnostic enzymes activity in blood (aminotransferase--AST 6 times and ALT 5.6 times, lactatedehydrogenase 4.9 times, gamma-glutamyltranspeptidase 3.6 times) and normalization of morphological state of the liver cells. The hepatoprotective activity of the cluster rhenium compound with adamanthyl ligands was confirmed in the model of acute toxic hepatitis. Introduction of this compound led to reduction of the concentration of MDA in homogenates of liver tissue (2 times), and in blood plasma (3.8 times); to reduction of levels of diagnostic liver enzymes in blood--AST and ALT 5.8 and 5.5 times respectively in comparison with control group. Some aspects of the mechanism of hepatoprotection were discussed, that included the presence of conjugated systems around the quadrupol rhenium-rhenium bond and alkyl radicals with significant positive inductive effects.

[Antioxidant properties of rhenium cluster complexes with butyric acid derivatives in blood plasma and erythrocytes]. / Antyoksydantni vlastyvosti klasternykh kompleksiv reniiu z deiakymy pokhidnymy maslianoï kysloty u plazmi krovi ta erytrotsytakh.

The antioxidant action of rhenium cluster complexes with isobutiric and gamma-aminobutiric acids were studied in vitro. It was established that strong antiradical properties were found in the reaction with stable radical diphenylpycrylhydrazile Re2(i-C3H7COO)4Cl2, [Re2(GABA)2Cl5(H2O)]Cl.2H2O. The chemiluminescence investigation of antioxidant properties of both substances Re2(i-C3H7COO)4Cl2 and [Re2(GABA)2Cl5(H2O)]Cl.2H2O has established that these properties are realized in the blood plasma and erythrocytes, but to different extent.

[Spectrophotometric study of the interaction between rhenium complexes and phosphatidylcholine during liposome formation]. / Spektrofotometrychne doslidzhennia vzaémodiï kompleksnykh spoluk reniiu z fosfastydylkholinom pry formuvanni liposom.

The electron absorption spectra of halogenides and halogencarboxylate complex compounds of rhenium (III) having cluster structure with phosphatydilcholine and their lyposome forms were investigated. Some results which evidence for the interaction of these compounds with phosphatydilcholine were obtained. The possible mechanism of this interaction is discussed.

Influence of cis-[Re2GABA2Cl4]Cl2 on the antioxidant defense system parameters of normal human blood.

The effect of the rhenium complex cis-[Re2GABA2Cl4]Cl2 on the antioxidant parameters of normal human blood in vitro have been studied. The results suggest that the complex influences various enzymes in the cascade of reactions utilizing active oxygen metabolites. However, the manifestation of this activity varies over the studied concentration range of the complex in the preincubation medium (10(-12)-10(-4) M), so the effects appear to be concentration-dependent. The largest differences in antioxidant parameters in comparison with control were observed for the concentrations 10(-8), 10(-5), and 10(-4) M. Thus, correlations between the peroxidation level, superoxide dismutase (SOD) activity, antioxidant factor (F), and indexes of resistance of erythrocytes for hemolysis (TR) were found.

[Pharmacological analysis of the antihypoxic action of aminobutyric acid derivatives]. / Farmakolohichnyi analiz antyhipoksychnoï diï pokhidnykh maslianoï kysloty.

The correlation-regressive and pharmacological analysis of butane acid derivates--ufibrat, Re2(isoC3H7COO)4Cl2Ta[Re2(GABA)2Cl4(H2O)2]Cl2--was made. It is established that among these substances antihypoxic activity on the hypercapnic hypoxic hypoxia have only ufibrat. It decrease mice's temperature of body, increase duration of period to the appearance of sudoms and the life longitudindity at the condition of hypercapnia-hypoxia.

[Effect of rhenium complexes with organic ligands on acid resistance of human erythrocytes]. / Izuchenie vliianiia kompleksov reniia s organicheskimi ligandami na kislotnuiu rezistentnost' éritrotsitov cheloveka.

The authors showed stabilizing effect of rhenium binuclear cluster compounds with organic ligands on erythrocytic membranes. Estimation of functional condition of cells was studied by a chemical fragility method. Biphase type of rhenium complexes concentration curves stabilization has been found out; the influence of organic radicals and axial substructures (Cl- and Br-) on degree of stabilization has been showed. Different mechanisms of interaction of the investigated complexes with erythrocytic are suggested.

[Spectral and thermographic characteristics of surface lipids from various plants]. / Spektral'na ta termogravimetrychna kharakterystyka poverkhnevykh lipidiv khvoï riznoho viku.

Spectral (IR) and thermogravimetrical properties of surface lipids of two coniferous species, differing during molecular weights of their components, were investigated in the process of ontogenic development. Different mechanisms of surface lipid formation in leaves of the investigated species are shown. It is supposed that low temperature endothermic effect, which is connected with phase transfer of the surface lipids amorphous part may play functional role in formation of stability of plants to gases.

[Investigation of oxosubstances in plant leaf lipids]. / Doslidzhennia oksospoluk poverkhnevykh lipidiv lystkiv roslyn.

Surface lipids of plant leaves of different groups were investigated by spectral, chromatographic and functional analysis methods. Absence of oxosubstances in surface lipids of coniferous and their characteristics in tropical species were shown. Functions of oxosubstances are discussed.

[Peculiarities in a composition of surface lipids in coniferous leaves]. / Osoblyvosti skladu poverkhnevykh lipidiv lystia khvoinykh.

Composition on surface lipids of coniferous from Picea, Pinus and Larix genera (six plant species) are studied by spectral and chromatographic methods. Different content of carbonyl compounds in the summary fraction of surface lipids of coniferous, concerning componental content of hydrocarbon fraction and predominance of concrete fatty acids was shown. A substantial quantity of unethereficated fatty acids in surface lipids of plants is shown.