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10.
Artigo em Russo | MEDLINE | ID: mdl-1087797

RESUMO

The authors describe a method of obtaining monospecific serum against secretory IgA and the corresponding standard. An immunochemically pure (11.6S) secretory human IgA was extracted from the colostrum by salt fractionation and gel-filtration through Sephadex G-200 and Sepharose 6B; this IgA was used as an antigen for the immunization and the standard for the quantitative determination of SIgA in the secretions. Monospecific anti-SC-serum was obtained by successive exhaustion of the antiserum against the S IgA immunosorbents prepared from normal human serum and the serum of a patient suffering from A myeloma containing polymeric IgA forms.


Assuntos
Anticorpos Anti-Idiotípicos , Imunoglobulina A , Anticorpos Anti-Idiotípicos/isolamento & purificação , Cromatografia em Gel , Colostro/imunologia , Feminino , Humanos , Imunoeletroforese , Imunoglobulina A/isolamento & purificação , Gravidez
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