RESUMO
Subchronic intoxications in rats induced by repeated intraperitoneal injections of stable water suspensions of silver or copper oxide nanoparticles in low dosage were manifested by adverse shifts in some functional and biochemical indices, by development of histo-structural changes in different tissues and by poly-organ fragmentation of DNA. All these manifestations of toxicity were substantially attenuated against the background of parallel oral administration of bioprotective complexes comprising vitamins, trace elements, pectin, some amino acids and a fish oil preparation rich in omega-3 fee fatty acids, this composition has been adjusted to mechanisms of action of this or that nanomaterial.
Assuntos
Poluentes Ocupacionais do Ar/toxicidade , Cobre/toxicidade , Intoxicação por Metais Pesados , Nanopartículas/toxicidade , Óxidos/toxicidade , Intoxicação/prevenção & controle , Compostos de Prata/toxicidade , Poluentes Ocupacionais do Ar/farmacocinética , Animais , Cobre/farmacocinética , Modelos Animais de Doenças , Masculino , Metais Pesados/metabolismo , Óxidos/farmacocinética , Intoxicação/metabolismo , Ratos , Compostos de Prata/farmacocinética , Distribuição TecidualRESUMO
Judging by the cytological characteristics of the free cell population of the lower airways obtained with assistance of the bronchoalveolar lavage in 24 hours after the intratracheal instillation of equal doses of equidimensional gold or silver nanoparticles, both metals result in active recruitment of phagocytes with domination of neutrophile leukocytes, especially marked after the instillation of the nanosilver. The higher ratio of these cells count to that of alveolar macrophages gives evidence for the significantly higher cytotoxicity of the nanosilver comparing with both nanogold and even the smallest silver particles in the micrometric range. Transmission electron microscopy demonstrates similar pictures of intracellular distribution and ultra-structural damages caused by internalized nanoparticles in both types of phagocytes, while there are significant differences between cells under impact of nanosilver vs. those under impact of nanogold. The highest importance is higher propensity of the nanosilver particles to aggregation and to ingression into mitochondria with damaging these organelles.
