RESUMO
The report discusses our experimental data in support of biotherapy which uses chemotherapy and antitumor immune treatment with in vivo xenogenic transfer-factor polypeptides (TFP) isolated from lymphocytes sensitized to antigens of given tumor. After excision of primary tumor--lung carcinoma of Lewis--mice C57BL/6 were injected intraperitoneally with xenogenic TFP (200 pg/body, twice) and a cytostaic dose of cyclophosphamide. Such adjuvant chemotherapy was found to prevent metastases from spreading to the lung in 100%. The marked anti-metastatic effect of the treatment correlated with recovery of splenic cell mass and its cellular structure, higher levels of large granular lymphocytes in peripheral blood and enhanced functional activity of cytotoxic cells in vitro. Our results point to a possibility of raising efficacy of treating solid malignancies with adjuvant chemotherapy in combination with adoptive immune therapy.
Assuntos
Carcinoma Pulmonar de Lewis/prevenção & controle , Ciclofosfamida/farmacologia , Imunossupressores/farmacologia , Imunoterapia Adotiva/métodos , Neoplasias Pulmonares/prevenção & controle , Fator de Transferência/farmacologia , Animais , Antineoplásicos Alquilantes/administração & dosagem , Antineoplásicos Alquilantes/farmacologia , Carcinoma Pulmonar de Lewis/secundário , Ciclofosfamida/administração & dosagem , Imunossupressores/administração & dosagem , Injeções Intraperitoneais , Neoplasias Pulmonares/secundário , Camundongos , Camundongos Endogâmicos C57BL , Peptídeos/farmacologia , Fator de Transferência/administração & dosagemRESUMO
Factor of transfer was identified and sampled in the course of immunization of rats with cells or tissues of mouse lung carcinoma of Lewis and its antitumor action investigated on models of passive and spontaneous dissemination in mice C57BL/6. Samples obtained at the peak of immunological response (day 14) and immunological memory inception (day 60) were shown to be capable of marked antimetastatic effects: fall in metastatic frequency, number and size of metastases correlated with higher index of metastasis formation.
Assuntos
Carcinoma Pulmonar de Lewis/imunologia , Carcinoma Pulmonar de Lewis/patologia , Imunização/métodos , Fator de Transferência/imunologia , Animais , Camundongos , Camundongos Endogâmicos C57BL , Ratos , Fatores de TempoRESUMO
Low-molecular extracts (LMEs) of lymphocytes were obtained from spleen of noninbred rats using our experimental model of intraorganic growth of Guerin's carcinoma. They were intended to transfer immune reaction to tumor antigens in vitro. It was LMEs developed prior to tumor progression in spleen that showed immunospecific activity with respect to tumor cells. Also, they had marked antigen-independent immunopharmacological activity. Single intravenous injection of LMEs100 pg given on day 7 of tumor growth stimulated antitumor resistance in intact rats within a short time. It prevented tumor cells engrafting in 60% of tumors. A tumor-specific factor has been evolved capable of immune reaction transfer to tumor antigens in vitro thus preventing tumorigenesis in recipients in vivo.