RESUMO
The influence of probiotic strains of Lactobacillus casei IMV B-7280, Lactobacillus acidophilus IMV B-7279, Bifidobacterium animalis VKL, Lactobacillus delbrueckii subsp. bulgaricus IMV B-7281 and Bifidobacterium animalis VKB, each strain separately, to the range of the urogenital tract microflora in physiological norm and in cases of experimental intravaginal staphylococcosis of mice induced by Staphylococcus aureus 8325-4. It was found that all these strains had different efficiency in Staphylococcus aureus 8325-4 growth suppression in the vagina of infected mice and affect the spectrum of microorganisms. Lactobacillus casei IMV B-7280 strain had effective antistaphylococcal effect and reduced the number of coliform bacteria and fungi in the vagina in cases of intravaginal staphylococcosis of mice. Lactobacillus casei IMV B-7280 strain is promising to create probiotic drugs effective in treating intravaginal staphylococcosis.
Assuntos
Probióticos/uso terapêutico , Infecções Estafilocócicas/tratamento farmacológico , Vagina/microbiologia , Vaginose Bacteriana/tratamento farmacológico , Administração Intravaginal , Animais , Antibiose , Contagem de Colônia Microbiana , Modelos Animais de Doenças , Feminino , Camundongos , Camundongos Endogâmicos BALB C , Probióticos/administração & dosagem , Probióticos/farmacologia , Infecções Estafilocócicas/microbiologia , Vagina/efeitos dos fármacos , Vaginose Bacteriana/microbiologiaRESUMO
The effect of intravaginal injection of Lactobacillus casei IMV B-7280, Lactobacillus acidophilus IMV B-7279, Bifidobacterium animalis VKL and Bifidobacterium animalis VKB strains in various compositions on the range of microflora of the urogenital tract on the model of experimental intravaginal staphylococcosis of mice was determined. It was established that under the influence of various compositions of these strains changes in microflora spectrum occurred: the number of representatives of normoflora increased and the number of pathogenic microorganisms, including staphylococci, streptococci, coliform bacteria and fungi, significantly decreased. It was determined that strains of lacto- and bifidobacteria, that were studied, are prospective components of future probiotic drugs efficient in treating staphylococcosis.
Assuntos
Bifidobacterium/fisiologia , Lactobacillus/fisiologia , Probióticos/farmacologia , Infecções Estafilocócicas/prevenção & controle , Staphylococcus aureus/efeitos dos fármacos , Vagina/microbiologia , Administração Intravaginal , Animais , Antibiose , Contagem de Colônia Microbiana , Modelos Animais de Doenças , Feminino , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Infecções Estafilocócicas/microbiologia , Staphylococcus aureus/crescimento & desenvolvimento , Staphylococcus aureus/patogenicidade , Vagina/efeitos dos fármacosRESUMO
The effect of intravaginal injection of Lactobacillus casei IMV B-7280, Lactobacillus acidophilus IMV B-7279, Bifidobacterium animalis VKL and Bifidobacterium animalis VKB strains in various compositions on the range of microflora of the urogenital tract on the model of experimental intravaginal staphylococcosis of mice was determined. It was established that under the influence of various compositions of these strains changes in microflora spectrum occurred the number of representatives of normoflora increased and the number of pathogenic microorganisms, including staphylococci, streptococci, coliform bacteria and fungi, significantly decreased. It was determined that strains of lacto- and bifidobacteria, that were studied, are prospective components of future probiotic drugs efficient in treating staphylococcosis.
Assuntos
Bifidobacterium/fisiologia , Lacticaseibacillus casei/fisiologia , Lactobacillus acidophilus/fisiologia , Probióticos/uso terapêutico , Infecções Estafilocócicas/terapia , Vagina/efeitos dos fármacos , Administração Intravaginal , Animais , Antibiose , Contagem de Colônia Microbiana , Modelos Animais de Doenças , Enterobacteriaceae/efeitos dos fármacos , Enterobacteriaceae/crescimento & desenvolvimento , Feminino , Fungos/efeitos dos fármacos , Fungos/crescimento & desenvolvimento , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Probióticos/administração & dosagem , Infecções Estafilocócicas/microbiologia , Staphylococcus/efeitos dos fármacos , Staphylococcus/crescimento & desenvolvimento , Streptococcus/efeitos dos fármacos , Streptococcus/crescimento & desenvolvimento , Vagina/microbiologiaRESUMO
Lactic acid bacteria and bifidobacteria were isolated from the samples of dry microbial substance "Vivolac" and identified. The lactic acid bacteria were identified as Lactobacillus acidophilus. L. delbrueckii subsp. bulgaricus, L. paracasei. Bifidobacteria belonged to the species Bifidobacterium animalis. Manifestation of biological activity of all studied strains was investigated. It was shown, that adhesive activity of the studied strains depended on the genus of the strain: lactobacilli manifested medium adhesiveness to buccal epithelium (medium adhesive index 2.07-2.26), bifidobacteria manifested high adhesiveness (medium adhesive index 4.11-4.76). Antagonism against pathogenic and opportunistic reference strains was manifested to a greater extent to Pseudomonas aeruginosa. Lactobacilli and Bifidobacteria were resistant to aminoglycosides. Sensitivity of the studied strains to antibiotics did not depend on the genus and species of microorganism. Lactobacillus and Bifidobacterium strains were capable to survive in gastric juice and at different concentration of bile, which is promising for development of probiotic preparations on the basis of these strains.
