Oxidation engineering triggered peroxidase-like activity of VOxC for detection of dopamine and glutathione.

MXenes, two-dimensional nanomaterials, are gaining traction in catalysis and biomedicine. Yet, their oxidation instability poses significant functional constraints. Gaining insight into this oxidation dynamic is pivotal for designing MXenes with tailored functionalities. Herein, we crafted VOxC nanosheets by oxidatively engineering V4C3 MXene. Interestingly, while pristine V4C3 displays pronounced antioxidant behavior, its derived VOxC showcases enhanced peroxidase-like activity, suggesting the crossover between antioxidant and pro-oxidant capability. The mixed valence states and balanced composition of V in VOxC drive the Fenton reaction through multiple pathways to continually generate hydroxyl radicals, which was proposed as the mechanism underlying the peroxidase-like activity. Furthermore, this unique activity rendered VOxC effective in dopamine and glutathione detection. These findings underscore the potential of modulating MXenes' oxidation state to elicit varied catalytic attributes, providing an avenue for the judicious design of MXenes and derivatives for bespoke applications.

Gbb Regulates Blood Cell Proliferation and Differentiation through JNK and EGFR Signaling Pathways in the Drosophila Lymph Gland.

The Drosophila lymph gland is an ideal model for studying hematopoiesis, and unraveling the mechanisms of Drosophila hematopoiesis can improve our understanding of the pathogenesis of human hematopoietic malignancies. Bone morphogenetic protein (BMP) signaling is involved in a variety of biological processes and is highly conserved between Drosophila and mammals. Decapentaplegic (Dpp)/BMP signaling is known to limit posterior signaling center (PSC) cell proliferation by repressing the protooncogene dmyc. However, the role of two other TGF-ß family ligands, Glass bottom boat (Gbb) and Screw (Scw), in Drosophila hematopoiesis is currently largely unknown. Here, we showed that the loss of Gbb in the cortical zone (CZ) induced lamellocyte differentiation by overactivation of the EGFR and JNK pathways and caused excessive differentiation of plasmatocytes, mainly by the hyperactivation of EGFR. Furthermore, we found that Gbb was also required for preventing the hyperproliferation of the lymph glands by inhibiting the overactivation of the Epidermal Growth Factor Receptor (EGFR) and c-Jun N-terminal Kinase (JNK) pathways. These results further advance our understanding of the roles of Gbb protein and the BMP signaling in Drosophila hematopoiesis and the regulatory relationship between the BMP, EGFR, and JNK pathways in the proliferation and differentiation of lymph gland hemocytes.

Chemical Potential-Modulated Ultrahigh-Phase-Purity Growth of Ultrathin Transition-Metal Boride Single Crystals.

Two-dimensional (2D) transition-metal borides (TMBs) are especially expected to exhibit excellent performance in various fields among electricity, superconductivity, magnetism, mechanics, biotechnology, battery, and catalysis. However, the synthesis of ultrathin TMB single crystals with ultrahigh phase purity was deemed extremely challenging and has not been realized till date. That is because TMBs have the most kinds of crystal structures among inorganic compounds, which possess generous phase structures with similar formation energies compared with other transition-metal compounds, attributing to the metalloid and electron-deficient characteristics of boron. Herein, for the first time, we demonstrate a chemical potential-modulated strategy to realize the precise synthesis of various ultrahigh-phase-purity (approximately 100%) ultrathin TMB single crystals, and the precision in the phase formation energy can reach as low as 0.01 eV per atom. The ultrathin MoB2 single crystals exhibit an ultrahigh Young's modulus of 517 GPa compared to other 2D materials. Our work establishes a chemical potential-modulated strategy to synthesize ultrathin single crystals with ultrahigh phase purity, especially those with similar formation energies, and undoubtedly provides excellent platforms for their extensive research and applications.

CSTNet: A Dual-Branch Convolutional Neural Network for Imaging of Reactive Flows Using Chemical Species Tomography.

Chemical species tomography (CST) has been widely used for in situ imaging of critical parameters, e.g., species concentration and temperature, in reactive flows. However, even with state-of-the-art computational algorithms, the method is limited due to the inherently ill-posed and rank-deficient tomographic data inversion and by high computational cost. These issues hinder its application for real-time flow diagnosis. To address them, we present here a novel convolutional neural network, namely CSTNet, for high-fidelity, rapid, and simultaneous imaging of species concentration and temperature using CST. CSTNet introduces a shared feature extractor that incorporates the CST measurements and sensor layout into the learning network. In addition, a dual-branch decoder with internal crosstalk, which automatically learns the naturally correlated distributions of species concentration and temperature, is proposed for image reconstructions. The proposed CSTNet is validated both with simulated datasets and with measured data from real flames in experiments using an industry-oriented sensor. Superior performance is found relative to previous approaches in terms of reconstruction accuracy and robustness to measurement noise. This is the first time, to the best of our knowledge, that a deep learning-based method for CST has been experimentally validated for simultaneous imaging of multiple critical parameters in reactive flows using a low-complexity optical sensor with a severely limited number of laser beams.

Adsorption-Free Growth of Ultra-Thin Molybdenum Membranes with a Low-Symmetry Rectangular Lattice Structure.

Although low-symmetry lattice structure of 2D transition metals is highly anticipated for both fundamental research and potentially distinctive application, it still has not been experimentally realized, which greatly hinders the exploration of the unique properties. Here, ultra-thin body-centered-cubic (bcc) phase molybdenum (Mo) membranes are successfully synthesized with a low-symmetry rectangular (110) crystal face via an adsorption-free reaction. Through experimental and density functional theory studies, no foreign atoms being adsorbed is shown to be a key factor for the successful preparation of the bcc phase 2D transition metal with (110) faces. The realization of 2D Mo(110) with a low-symmetric rectangular lattice structure extends the scope of 2D structures and is also beneficial for the exploration and development of low-symmetry rectangular lattice-structured materials with unique properties.

Lysosome and proteasome pathways are distributed in laticifers of Euphorbia helioscopia L.

At present, the lysosome pathway (LP) and proteasome pathway (PP) are known as major clearance systems in eukaryotic cells. The laticifer, a secretory tissue, degrades some cytoplasm during development. In this study, we investigated the distribution of LP and PP in non-articulated laticifers of Euphorbia helioscopia L. Electron microscopy revealed that, plastids, mitochondria and some cyotsol were degraded in the late development laticifers, where there were numerous vesicles originated from dicytosomes. Accordingly, some key proteins in LP and PP were detected in E. helioscopia latex using isobaric tags for relative and absolute quantitation (iTRAQ) proteomics. Further immunohistochemistry analysis revealed that the clathrin heavy chain (CHC) belonging to LP and the ubiquitin-mediated proteasome degradation increases gradually as the laticifer develops. Immuno-electron microscopy revealed that the cysteine protease, CHC and AP-2 complex subunit beta-1 belonging to LP were mainly distributed in vesicles deriving from dicytosomes, which we called lysosome-like vesicles. Ubiquitin was widely distributed in the cytosol, and proteasome activity was significantly reduced when various concentrations of the inhibitor MG132 were added to the latex total protein. We hypothesize that LP and PP are distributed in E. helioscopia laticifers; and it was speculated that LP and PP might be involved in the degradation of organelles and some cytoplasmic matrix in E. helioscopia laticifers.

Sub-nanometer Co3O4 clusters anchored on TiO2(B) nano-sheets: Pt replaceable Co-catalysts for H2 evolution.

Cobalt-based heterogeneous cocatalysts are important substitutions of noble metal cocatalysts in many important commercial chemical processes, but their efficiency is extremely low on a per metal atom basis, because only the atoms located at surface active-sites participate in the chemical reaction. Thus, cocatalysts with small cluster dispersions are highly desirable to maximize the amount of active-sites and enhance the per atom efficiency. Here, we report the synthesis of sub-nanometer Co3O4 clusters which are anchored to 2D ultrathin TiO2(B) nanosheets, as a cocatalyst for H2 evolution reaction (HER). It was found that the conduction type of Co3O4 clusters turns from P-type to N-type, and the heterojunction band structure between TiO2(B) and Co3O4 clusters changes from type II to type I, when the cluster size is reduced from nanometer scale to the sub-nanometer scale. With a suitable energy band matching between TiO2(B) and sub-nanometer Co3O4 clusters, the electrons generated in TiO2(B) during the photocatalytic process reduce the Co ions into metallic Co atoms, which produce excellent photocatalytic stability and extremely high HER efficiency comparable to that of the noble Pt cocatalyst.

Comparative proteomics of Euphorbia kansui Liou milky sap at two different developmental stages.

Euphorbia kansui Liou is a unique traditional Chinese medicinal herb. Its milky sap proteins play important roles in laticifer development, synthesis and transport of its biologically active substances. A proteomic approach was applied to analyze the E. kansui latex proteins related to laticifer development and secondary metabolite synthesis by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and two-dimensional polyacrylamide gel electrophoresis. A total of 125 milky sap proteins associated with development of laticifers, disease and defense, and general metabolism were identified, and 19 differentially expressed proteins at two different developmental stages of laticifers were successfully detected. Peroxidase, cytochrome P450 mono-oxygenase superfamily, lipoxygenase, and multidrug resistance protein ABC transporter family may be involved in laticifer development, secondary metabolite synthesis and transport, and plant physiology.

Immunocytochemical localization of saikosaponin-d in vegetative organs of Bupleurum scorzonerifolium Willd.

BACKGROUND: Saikosaponin-d (SSd) is an important active component of Bupleurum scorzonerifolium Willd., a traditional Chinese medicinal herb. Thus far, the biosynthetic pathway and biosynthetic site of saikosaponins in Bupleurum are largely unknown. The cellular localization of SSd will help in understanding saikosaponin biosynthesis and regulation. RESULTS: In this study, we characterize for the first time the localization of SSd in B. scorzonerifolium tissues and cells using histochemistry and immunoelectron microscopy. The results show that the saikosaponin distribution in different plant organs changes as they mature. The number of SSd gold particles distinctly differed among the roots, stems, and leaves, with the particles mainly concentrated in the roots. The gold particles were mainly observed in vacuoles, with a few particles in the protoplasm; hence, SSd is mainly stored in vacuoles. CONCLUSIONS: We speculate that saikosaponins are mainly synthesized via the mevalonate pathway in the protoplasm in young organs, and then transported to the central vacuole by the endoplasmic reticulum (ER) or the fusion of vacuoles, to protect plants from self-poisoning with the accumulation of more saikosaponins.