Assuntos
Brassica/fisiologia , Eletroporação/métodos , Plantas Geneticamente Modificadas , Brassica/genética , Células Cultivadas , Meios de Cultura , Expressão Gênica , Técnicas de Transferência de Genes , Glucuronidase/biossíntese , Glucuronidase/genética , Indicadores e Reagentes , Canamicina Quinase , Fosfotransferases (Aceptor do Grupo Álcool)/biossíntese , Fosfotransferases (Aceptor do Grupo Álcool)/genética , Plasmídeos , Protoplastos/fisiologia , Proteínas Recombinantes/biossíntese , Transfecção/métodos , Transformação GenéticaRESUMO
The paper compares different approaches for the genetic transformation of cauliflower (Agrobacterium-mediated, PEG-mediated and/or electroporation). Transient expression of the neomycin phosphotransferase II (NPTII) gene could be detected after direct gene transfer. Stable transformation was achieved using both Agrobacterium-mediated and direct gene transfer. Expression as well as incorporation of the NPTII sequence could be demonstrated.
Assuntos
Brassica/genética , Fosfotransferases/genética , Rhizobium/genética , Transfecção , Transformação Genética , Canamicina Quinase , Fosfotransferases/isolamento & purificação , Fosfotransferases/metabolismo , Plasmídeos , Protoplastos/enzimologiaRESUMO
The paper describes scanning electron microscopic observations of electric field induced fusions of human lymphocytes. The cells were fused by application of short-time (20 microseconds) field pulses of 5 kV/cm after dielectrophoretic collection at about 1.2 MHz and 750 V/cm. Electron microscopy clearly demonstrates membrane fusion and the stability of initial stages of fusion.
Assuntos
Fusão Celular , Linfócitos/ultraestrutura , Membrana Celular/ultraestrutura , Humanos , Microscopia Eletrônica de VarreduraRESUMO
The fusion of human cells of the cerebrospinal fluid and of the peripheral blood is reported, as well as the fusion of these cells with tomato protoplasts. The cells were fused by applying short-time electric pulses after dielectrophoretic collection. The importance of this method for diagnosis and therapy is discussed and possible applications are mentioned.