[Estimation of selected parameters antioxidant barrier and lipid parameters in children with atherosclerosis risk factors]. / Ocena wybranych parametrów bariery antyoksydacyjnej i przemiany lipidowej u dzieci ze stwierdzonymi czynnikami ryzyka miazdzycy.

UNLABELLED: Atherosclerosis is one of the most common causes of mortality in developed countries. One of the many factors in its etiopathogenesis are reactive oxygen species (ROS), excess of which comes from disturbances of enzymatic and nonenzymatic mechanisms of antioxidant barrier. THE AIM OF THIS STUDY was to estimate relations between the activity of chosen parameters antioxidant barrier and the lipid levels in children with atherosclerosis risk factors. MATERIAL AND METHODS: The study was carried out on 48 children with atherosclerosis risk factors. The control group consisted of 25 healthy children. Total cholesterol (TC), Triglycerides (TG), HDL-C, LDL-C were determined by enzymatic method. The activity of superoxide dismutase (SOD) was estimated by Misra and Fridivich's method, glutathione peroxidase (GSH-Px) with method proposed by Sedlak and Lindsay in modyfication Little and Brien and the activity o catalase (CAT) by Beer and Sizer's method. Malonyl dialdehyde (MDA) concentration was determined by Placer's method. RESULTS: Obesity, lipid disorders, and hypertension were the most frequent risk factors in the investigated children. Statistically significant higher concentration of TC, LDL-C, TG and lower HDL-C were observed in children with atherosclerosis risk factors. Activity of glutathion peroxidase was statistically significant higher in children with atherosclerosis risk factors (p < 0.001), activity of catalase was lower (p < 0.05). No significant differences in activity of superoxide dismutase and malonyl dialdehyde were observed in the investigated groups. CONCLUSION: Modificated activity of selected antioxidants in children with atherosclerosis risk factors could determine an index of antioxidant barrier and might potencially constitute the marker of early atherosclerotical risk development.

[Estimation of relation between homocysteine concentration and selected lipid parameters and adhesion molecules concentration in children with atherosclerosis risk factors]. / Ocena zaleznosci pomiedzy stezeniem homocysteiny a stezeniem wybranych parametrów przemiany lipidowej i czasteczek adhezyjnych u dzieci z czynnikami ryzyka miazdzycy.

UNLABELLED: Atherosclerosis begins in childhood. At present among numerous risk factors of atherosclerosis the role of hiperhomocysteinemia in development of cardiovascular heart disease is taken under consideration. Atherogenic effect of homocystein is related to its cytotoxin action, conducting to endothelial dysfunction and damage. It is correlated with increase of the lipid levels in the blood serum and change of expression of the soluble forms of adhesion molecules. The aim of this study was to estimate relations between the homocystein serum concentration, expression of the selected adhesion molecules and the lipid levels in the blood serum in children with atherosclerosis risk factors. MATERIAL AND METHODS: The group consisted of 670 children, 76 of them had atherosclerosis risk factors. In further examination 48 children have taken a part, whose parents were agreed for theirs participation in the program. The comparative group composed of 25 children without the risk factors. We determined total cholesterol (TC), triglycerides (TG), LDL cholesterol fraction (LDL-C), HDL cholesterol fraction (HDL-C), serum homocysteine concentration (Hcy), the expression of the soluble forms of adhesion molecules (sCAM): sP-selectin and sVCAM-1 (vascular cell adhesion molecule-1). RESULTS: Obesity, hypertension and lipid disorders in the shape of higher concentration of TC, LDL-C, TG and lower HDL-C were the most frequent risk factors in the investigated children. No significant differences in serum homocysteine concentration were observed between the investigated groups. However, its concentration was significantly higher in children with two atherosclerosis risk factors. No significant differences in expression of s-VCAM-1 were observed in the investigated groups, concentration of sP-selectin was significantly higher in children with atherosclerosis risk factors (p<0.05). Statistically significantly higher serum concentrations of lipid levels were found in children with hyperhomocysteinemia. CONCLUSION: Higher concentration of the serum homocysteine and chosen adhesion molecules in children with atherosclerosis risk factors might potentially constitute the marker of early atherosclerotical risk development.

[Homocystein serum levels and lipid parameters in children with atherosclerosis risk factors]. / Homocysteina i wybrane parametry przemiany lipidowej u dzieci z czynnikami ryzyka miazdzycy tetnic.

UNLABELLED: Atherosclerosis is a disease of adult patients, however, it begins in childhood and progresses from fatty streaks to raised lesions in arteries in adolescence and young adults. Clinical manifestation of atherosclerosis in adulthood depends on the risk factors such as: lipid disorders, obesity, hypertension, smoking habits and family history of CHD. High serum homocysteine concentration is increasingly recognised as a new risk factor for atherosclerosis and other vascular diseases. Atherogenic effect of homocystein is related to cytotoxin action on the endothelial cells and their function. The aim of this study was to estimate relations between the homocysteine serum concentration and the lipid levels in children with atherosclerosis risk factors. MATERIAL AND METHODS: The study was carried out on 48 children with atherosclerosis risk factors. The control group consisted of 25 healthy childrens. Total cholesterol (TC), Triglycerides (TG), HDL-C, LDL-C were determined by enzymatic method. Concentration of homocysteine was estimated by immunoenzymatic method (ELISA). RESULTS: Obesity, lipid disorders, and hypertension were the most frequent risk factors in the investigated children. Statistically significant higher concentration of TC, LDL-C, TG and lower HDL-C were observed in children with atherosclerosis risk factors. No significant differences in homocystein concentration were observed in the investigated groups, but homocystein concentration was significantly higher in group of children with atherosclerosis risk factors. CONCLUSION: We observed that increased number of the risk factors is followed by high homocystein concentration in the serum.

[Assessment of 8-hydroxy-2'-deoxyguanosine concentrations in bladder cancer patients treated with intravesical BCG instillation]. / Analiza stezenia 8-hydroksy-2'-deoxyguanozyny w moczu chorych na powierzchownego raka pecherza moczowego leczonych zawiesina pratka BCG.

UNLABELLED: Increased 8-hydroxy-2'-deoxyguanosine (8-OHdG) concentration has been proposed as reliable marker of the oxidative DNA damage, and prognostication of urological carcinogenesis, particularly in bladder cancer. Widely accepted method of treatment in early stages of bladder cancer is transurethral electro resection (TURN). Intravesical bacillus Calmette-Guerin instillation is considered to be the therapeutic agent for superficial transitional cell carcinoma (TCC) of the bladder and has been established as standard therapy in the patients. The aim of the study was to test the concentration of 8-OHdG in urine in patients with bladder cancer and the effect of TURN and BCG therapy on the 8-OHdG value. MATERIAL AND METHODS: We tested 12 patients (3 female and 9 male) with superficial bladder cancer and 31 healthy controls. Urine for the examination was drawn in 4 time points: before and 2 weeks after TURN treatment, then 6 weeks after 6 intravesical instillations of BCG (Onko BCG--BIO MED Lublin) by Morales and 12 weeks after TURN. 8-OHdG concentration in urine was tested using ELISA commercial kit (OXIS health) and the values of 8-OHdG are expressed as ng/ml of urine. RESULTS: Patients with superficial bladder cancer had 16.89 ng/ml of 8-OHdG in urine before the TURN procedure. The value was significantly (p< 0.005) higher then 12.98 ng/ml noted in healthy controls. 2 weeks after the procedure the 8-OHdG level decreased to 13.36 ng/ml. After 6 weeks of repeated 6 intravesical instillations of the BCG the concentration of 8-OHdG dropped to 10.91 ng/ml (p<0.005) and returned to the value 13.28 ng/ml after the next 4 weeks. CONCLUSIONS: Patients with bladder cancer have significantly increased concentration of 8-OHdG in urine compared to controls. 8 week combine therapy with TURN and BCG resulted in a significant decrease in 8-OHdG concentration in urine The beneficial effect of BCG instillations seems to result from strengthening of the antioxidative DNA protection.