Dynamics of the Enteric Virome in a Swine Herd Affected by Non-PCV2/PRRSV Postweaning Wasting Syndrome.

A commercial pig farm with no history of porcine circovirus 2 (PCV2) or porcine reproductive and respiratory syndrome virus (PRRSV) repeatedly reported a significant reduction in body weight gain and wasting symptoms in approximately 20-30% of the pigs in the period between three and six weeks after weaning. As standard clinical interventions failed to tackle symptomatology, viral metagenomics were used to describe and monitor the enteric virome at birth, 3 weeks, 4 weeks, 6 weeks, and 9 weeks of age. The latter four sampling points were 7 days, 3 weeks, and 6 weeks post weaning, respectively. Fourteen distinct enteric viruses were identified within the herd, which all have previously been linked to enteric diseases. Here we show that wasting is associated with alterations in the enteric virome of the pigs, characterized by: (1) the presence of enterovirus G at 3 weeks of age, followed by a higher prevalence of the virus in wasting pigs at 6 weeks after weaning; (2) rotaviruses at 3 weeks of age; and (3) porcine sapovirus one week after weaning. However, the data do not provide a causal link between specific viral infections and the postweaning clinical problems on the farm. Together, our results offer evidence that disturbances in the enteric virome at the preweaning stage and early after weaning have a determining role in the development of intestinal barrier dysfunctions and nutrient uptake in the postweaning growth phase. Moreover, we show that the enteric viral load sharply increases in the week after weaning in both healthy and wasting pigs. This study is also the first to report the dynamics and co-infection of porcine rotavirus species and porcine astrovirus genetic lineages during the first 9 weeks of the life of domestic pigs.

Use of paracetamol in sows around farrowing: effect on health and condition of the sow, piglet mortality, piglet weight and piglet weight gain.

BACKGROUND: Pain and fever in the periparturient period can lead to prolonged farrowing and can slow down the recovery of the sow, which will have an effect on the vitality and survival of the piglets. This study investigated the use of orally administered Paracetamol (Pracetam- CEVA) in sows in the periparturient period. RESULTS: Mortality did not differ in piglets during the first week, or during total lactation (P > 0.10). No difference was found in weight or weight gain of the piglets during lactation. The coefficient of variation of piglet weight was smaller in the Paracetamol (Pm) group at day 7, day 14 and at weaning, but not at birth. So, the variation within litters was smaller in the Pm- treated sows, in comparison with the Control (C) litters. No difference in mean IgG concentration was found between treatments, but the coefficient of variation was too high (> 40) in 50% of the C litters and not in Pm litters. The Pm- treated sows lost less backfat than the C- sows. No effect was found on the body temperature of the sows, but fever was rare in both groups. CONCLUSION: Paracetamol results in less variation of body weight of piglets during lactation, seems to have a potential effect on the distribution of IgG within litters and has a positive effect on backfat loss. The effects of Paracetamol might be even more pronounced in farms with high piglet mortality (this farm only 8%) or with a high incidence of agalactia, fever after farrowing or piglet diarrhoea, which was not the case in this farm. Paracetamol is a promising product for increasing the welfare in lactating sows and optimising production in the farrowing stable.

Dynamics of faecal shedding of ESBL- or AmpC-producing Escherichia coli on dairy farms.

OBJECTIVES: To explore the dynamics of faecal ESBL/AmpC shedding in dairy cattle and farmers, a study was conducted to examine changes in shedding by individual animals, as well as environmental exposure, and to study the association between antimicrobial use (AMU) and ESBL/AmpC shedding. METHODS: The study comprised a cross-sectional survey of 20 farms and a 1 year follow-up of 10 farms. Faecal samples were cultured by both direct inoculation on MacConkey agar + 1 mg/L cefotaxime (MC+) and enrichment in LB-broth + 1 mg/L cefotaxime with subsequent inoculation on MC+. Dust samples were collected using electrostatic dustfall collectors (EDCs). Human faecal samples were collected by the farmers. Presence of ESBL/AmpC genes was screened for by PCR and sequencing. Using mixed effects logistic regression, ORs were determined and population-attributable fractions (PAFs) calculated subsequently. RESULTS: In Phase 1, 8/20 farms were positive for ESBL/AmpC and, with 2 negative farms, were selected for Phase 2. Transient shedding of dominant allele variants was observed in the animals. EDCs and human faecal samples did not reflect what was observed in the animals. AMU was related to shedding of ESBLs in the next sampling moment [OR 14.6 (95% CI 3.0-80.0)] and the PAF of AMU was 0.36 (95% CI 0.08-0.77). Calves fed with colostrum from cows on dry-off therapy was not a risk factor [OR 1.7 (95% CI 0.7-4.9, P = 0.28)]. CONCLUSIONS: The presence of ESBL/AmpC could only be partly explained by AMU. No link was shown between shedding in cattle and humans or the environment. Interventions should focus on prevention of introduction.