Mechanism research of Maxing-Ersan Decoction in the treatment of COPD based on network pharmacology / 国际中医中药杂志

Objective:To explore the targets and signal pathways of Maxing-Ersan Decoction in the treatment of COPD by using network pharmacology, and to reveal the mechanism of intervention of multiple targets and multiple pathways of Maxing-Ersan Decoction in the treatment of COPD. Methods:The active ingredients and corresponding targets of Maxing-Ersan Decoction were screened with the help of TCMSP database, and the targets related to COPD disease were screened with GeneCards database. The STRING database was used for protein-protein interaction (PPI) network analysis. By using Cytoscape to build compound target network and PPI network; by using Draw Venn Diagram website to draw a Venn diagram, and using R software to perform gene ontology (GO) functional enrichment analysis and Kyoto encyclopedia of genes and genomes (KEGG). Results:A total of 101 active ingredients were obtained, with 250 corresponding targets, including 214 targets related to COPD. Through GO and KEGG enrichment analysis, all together 48 signal pathways related to the main components of Maxing-Ersan Decoction were screened out. Conclusion:Based on the network pharmacological analysis, the effective chemical components of Maxing-Ersan Decoction were found, and it was found that Maxing-Ersan Decoction may act on COPD through multiple targets and multiple pathways such as inflammation and immune regulation.

Application of sixminute walk test in the evaluation of children and adolescents cardiorespiratory endurance / 中国学校卫生

Abstract@#Cardiopulmonary endurance is an important indicator of the physical health of children and adolescents. The 6 Minute Walk Test(6MWT) is an emerging method used to assess the cardiorespiratory endurance of children and adolescents, and has been applied in many countries and regions. This study aims to review the application of 6MWT in the assessment of cardiopulmonary endurance in children and adolescents, analyze the application prospects and precautions of 6MWT, and provide references for the application and promotion of 6MWT in the assessment of cardiopulmonary endurance in children and adolescents, as well as the formulation of related evaluation standards.

Observation on curative effects of neoadjuvant chemotherapy for different subtypes of Luminal B breast cancer and analysis on its prognosis / 吉林大学学报(医学版)

Objective:To investigate the differences in the curative effects of neoadjuvant chemotherapy(NAC) for different subtypes of Luminal B breast cancer and its prognosis,and to discuss the clinical treatment characteristics of different subgroups.Methods:A total of 246 cases of Luminal B like breast cancer patients who completed the projected NAC courses and surgical treatment were selected.All the biopsy specimens before treatment were positive for estrogen receptot(ER).According to the expressions of progesterone receptor(PR), human epidermal growth factor-2(Her-2)and cell proliferation nuclear antigen Ki-67,246 cases of Luminal B breast cancer patients were divided into 3 subgroups.A subgroup(PR low expression group),Her-2 negative and PR< 20% or negative,Ki-67 any levels;B subgroup(PR high expression group),Her-2 negative,PR≥20% and Ki-67 ≥14%;C subgroup(Her-2 positive group),Her-2 positive,Ki-67 and PR any levels.The clinical pathological materials and follow-up recurrence events of the patients were collected.Results:Among the three subtypes of Luminal B breast cancer,there were no significant differences in the age,the size of primary tumor and the stage of TNM(P>0.05).There were significant differences in the lymph node metastasis rate among three subgroups(P=0.018),and the lymph node metastasis rate was the highest in A subgroup among three subgroups. There were no significant differences in the clinical response and pathological response among three subgroups(P=0.123,P=0.06).8.5%(21/246)patients achieved the pathological complete response(pCR);the rates of pCR in three subgroups had statistically significant difference(P=0.009);the rate of pCR in C subgroup was the highest, and the rate of pCR in B subgroup was the lowest.The Log-Rank test of the survival curves of three subgroups had not statistically significant difference(P=0.216),but the 3-year disease-free survival(DFS)and 5-year DFS of the patients in B subgroup were slightly higher than those in other two groups.The DFS of the patients in C subgroup was longer than that of the patients with Her-2 overexpression breast cancer at the same period,and the difference was statistically significant(P=0.047).Conclusion:Her-2 positive Luminal B breast cancer is more likely to achieve pCR in NAC and the prognosis is better than Her-2 overexpressing breast cancer.The patients with high expression of PR in Luminal B breast cancer patients have a tend of overall survival advantage compared with the patients with PR low expression and Her-2 positive expression.

Analysis of antibiotic resistance of gram-negative bacteria in PICU / 中国小儿急救医学

Objective To investigate the antibiotic resistance of gram-negative bacteria in PICU, to provide relevant background information for the prevention and treatment of nosocomial infection. Methods The basic clinical data of all children admitted to our hospital from January 2013 to December 2016 were col-lected,and the gram-negative bacteria isolated from PICU were retrospectively analyzed. Results A total of 757 strains of pathogenic bacteria were isolated in PICU during the past 4 years, including 509 strains of gram-negative bacteria, accounting for 67. 2％ of the total pathogens. The first five strains of gram-negative bacteria were Pseudomonas aeruginosa, Acinetobacter baumannii, Stenotrophomonas maltophilia,Klebsiella pneumonia and Escherichia coli. Positive specimens were mainly from lower respiratory tract and bloodstream infection. The antibiotic resistance analysis showed that gram-negative bacteria was generally resistance to ampicillin, ceftriaxone and cefazolin. Compared with other strains, Acinetobacter baumannii had higher resistance rate to common antibiotics, and the isolation rate of multi-drug resistant strains was more than 50％. Stenotrophomonas maltophilia was still sensitive to ceftazidime, levofloxacin and sulfamethoxazole. A total of 97 strains of multidrug-resistant gram-negative bacteria were isolated. Conclusion The majority of nosocomial infections in PICU were gram-negative bacteria, most of which came from lower respiratory tract infection, and the top five were Pseudomonas aeruginosa, Bauman Acinetobacter, Stenotrophomonasmaltophil-ia, Klebsiellapneumoniae and Escherichia coli. The emergence of multi-drug resistant bacteria and extensively drug-resistant bacteria indicates that the situation of drug resistance of gram negative bacteria is still grim.

Research advances of bronchiolitis obliterans in children / 国际儿科学杂志

Bronchiolitis obliterans (BO) is a uncommon but severe chronic obstructive pulmonary disease in children.The infections are the most common causes in children.Its clinical manifestations are recurrent or persistent cough,shortness of breath,wheezing,crackles.The diagnosis relies on clinical manifestations,lung function ard high-resolution CT.There is no standard treatment,but experiential therapy.The article reviews the progress on laboratory examinations,diagnosis and treatment of BO.

Craniomaxillofacial Images Analysis of Children with Ectodermal Dysplasia / 中山大学学报(医学科学版)

[Objective]To study the dental status,the development of jaw and the size of sella turcica of children with ectoder-mal dysplasia(ED).[Methods]Panaramic radiography and lateral cephalograms of six ED individuals(age range of 6~7 years,five males and one female)were obtained. The dental status was record. 17 measurements about the jaws and the sella turcica were mea-sured and compare them to Chinese children without ED syndrome.[Results]The mean number of missing teeth was 22.3 in perma-nent dentition and 16.2 in primary dentition;The teeth that most likely to absent were permanent lateral incisor ,maxillary first premo-lar,maxillary primary lateral incisor and mandibular primary central incisor,and all remaining teeth are in conical shape. Lateral cephalometric measurements showed that all ED subjects had lower ANS-Ptm,which suggested a short maxilla. Low Co-Po,ANB, NA-PA,N-Me,N-ANS and ANS-Me values that were found in all subjects,as well as low SNA,Y-axis,MP-FH,S-Co,and high SNB,NP-FH,NP-FH that were noted in some subjects showed counterclockwise rotation and protrusion of mandible with short-er length in ED subjects. Some subjects had low ANS-Me/N-Me × 100%and high N-ANS/N-Me × 100%,representing a short facial height. Five cases represented lower length and diameter of sella turcica;two cases showed lower depth of sella turcica ,indicating the abnormal development of sella turcica.[Conclusion]The results of this study suggest that the dentition ,jaws and sella turcica in ED children differs when compared to individuals without this syndrome.

Evaluation on efficacy of trastuzumab combined with neoadj uvant chemotherapy in Her-2 positive breast cancer patients and analysis of influencing factors of prognosis / 吉林大学学报(医学版)

Objective:To assess the efficacy of trastuzumab combined with neoadjuvant chemotherapy in the treatment of human epidermal growth factor receptor-2 (Her-2)positive breast cancer patients,and to explore its influencing factors of prognosis of breast cancer.Methods:The clinical materials of 112 Her-2 positive breast cancer patients were collected and they were divided into combined treatment group (trastuzumab + neoadj uvant chemotherapy)and single chemotherapy group (without trastuzumab ). SPSS 1 9.0 software was employed to calculate and analyze their clinical characteristics.The survival rate and prognosis were analyzed by Kaplan-Meier method,Log-rank test, and Cox regression. Results:Among the 112 Her-2 positive breast cancer patients, 23 cases were treated with trastuzumab,89 cases were treated without trastuzumab.The disease-free survival (DFS)in combined treatment group and single chemotherapy group had significant difference (P=0.012).And there was no significant difference of overall survival (OS)between two groups (P=0.064).The HR negative group had 18 (32.7%)patients with 5-level of Miller and Payne (MP)classification which was higher than HR positive group (5/46,10.9%)(P=0.009).In univariate analysis,the tumor size,node status at diagnosis and node status after operation were the influencing factors of DFS in the Her-2 positive breast cancer patients;the tumor size,node status at diagnosis,MP classification and node status after operation were the influencing factors of OS in the Her-2 positive breast cancer patients.The result of multivariate analysis indicated that the tumor size, node status at diagnosis,and with or without radiotherapy were the independent influencing factors of DFS and OS in the Her-2 positive breast cancer patients. Conclusion:The breast lesions in the Her-2 positive breast cancer patients with negative HR are relieved after treated with trastuzumab combined with neoadjuvant chemotherapy. The combination of trastuzumab and neoadj uvant chemotherapy can significantly improve the DFS and has a positive influence in the prognosis of Her-2 positive breast cancer patients.

The characteristic features of CT and MRI in the pancreatic neuroendocrine tumor / 医学研究生学报

Objective Pancreatic neuroendocrine tumor( PNET) is a rare kind of tumor with slow growth for which surgery is the main treatment.The histological grading of PNET was closely related to the choice of operation mode and the comprehensive treat-ment after operation.Imaging examinations such as enhanced CT and MRI are of great value in the preoperative diagnosis of PNET. The aim of this study was to investigate the characteristic features of CT and MRI in the PNET. Methods Preoperative imaging data of 80 patients with PNET confirmed by pathology were reviewed, including CT and MRI, and the image features of each PNET were an-alyzed and the statistical analysis was performed. Results There were significant differences in the lesion morphology between the G1 and G2 levels of PNET.The majority of forms of the G1 stage PNET lesions presented round-like regular shape, but the G2 stage did not show regular shape.CT examination showed that regular morphological lesions accounted for 91.30%(21/23) in the G1 level, and 52.00%(13/25)in the G2 level,χ2 =8.857,P=0.003.MRI examination showed that regular morphological lesions accounted for 88.48%(22/27)in the G1 level, and 55%(11/20) in the G2 level, χ2 =3.862,P=0.050.The difference of scan density in PNET lesions was statistically significant.Low density lesions accounted for 73.91%(17/23)in the G1 level, and 32%(8/25)in the G2 level.Equidensity lesions accounted for 26.09%(6/23)in the G1 level, and 68.00%(17/25)in the G2 level,χ2 =8.842,P=0.004.The difference of bile pancreatic duct expansion in the G1 and G2 level lesions was statistically significant by MRI examination. Lesions with expanded pancreatic duct accounted for 7.41%(2/27)in the G1 level,and 40%(8/20) in the G2 level, χ2 =7.287, P =0.007.There was significant difference in the peak time after en-hanced scanning between the G1 and G2 levels.Lesion enhancement peak at the arterial phase by CT examination of accounted for 21.74%(5/23) in the G1 level,and 64%(16/25) in the G2 level,χ2 =8.694,P=0.003.Lesion enhancement peak at the arterial phase by MRI examination accounted for 29.63% (8/27) in the G1 level, and 70% (14/20) in the G2 level, χ2 =7.521,P=0.006. Conclusion The lesion morphology, density of CT plain scan, CT or MRI enhancement peak time, and expansion of the bile duct and pancreatic duct can provide reliable information to distinguish the G1 and G2 stage in PNET.

The clinicophatholgic report of antivirus therapy in patients with liver cirrhosis resulting from Hepatitis B / 中国综合临床

Objective To evaluate the clinicophatholgic benefits and safty of antivirus therapy in patients with liver cirrhosis resulting from hepatitis B.Methods 80 patients with HBV-ralated liver cirrhosis were divided into three groups by the histopathology of liver:group of lamivudine treated with lamivudine 100 mg once daily;Adefovir group treated with Adefovir 10 mg once daily;control group treated with liver protective treatment only.Liver and renal function,PTA and HBV DNA were regularly measured.The Child push-Turotte sore and histopathology wag compared before and after treatment.All courses of treatment were 36 weeks.Results The scores of Child Pugh-Turotte sore in groups of lamivudine and Adefovir were lowered sinificantly (3.9 and 2.1 respectively),the load of HBV-DNA was decreased also[(4.1±0.9) copies/ml and(2.8±1.0) copies/ml],liver inflammmation decreased by more than 2 scores and liver fiber was improved by more than one score,with obviously significant difference(P<0.05) as compared with control group.Conclusion Patients with HBV-related cirrhosis treated with lamivudine and adefovir for antivims are improved and antivirus is important and safe to those during cirrhosis decompensation.

Expressions of progesterone receptor A and B in breast cancer and adjacent non-malignant tissues and significances / 吉林大学学报(医学版)

Objective To study the expressions of progesterone receptor A (PRA) and B (PRB) in breast cancer and adjacent non-malignant tissues and the correlations between their expressions and the clinical characteristics. Methods The expressions of PRA and PRB in 50 specimens of female human breast cancer and adjacent non-malignant tissues were detected by immunohistochemistry. The correlations between the expressions of PRA and PRB and the clinical characteristics were analyzed.Results PRA and PRB expressed in both the nuclei and the cytoplasma of tumor cells and epithelial cells of the acini and ducts.The percentages of PRA and PRB positive cells were 42%,42% and 52%,36% in the cancer and the adjacent non-malignant tissues,respectively,there was no significant difference.The expression of PRA was significantly correlated with the age of patient(r=-0.316 8,P

Application of Aiyishu injection in adjuvant treatment of breast cancer after operation and curative effect evaluation / 吉林大学学报(医学版)

Objective To evaluate the effect of Aiyishu injection on the cellular immunity state of body and toxic and side reaction in post-operation patients with breast cancer after chemotherapy .Methods Seventy patients with breast carcinoma at stage Ⅰ-ⅢA were randomly divided into treatment group (Aiyishu associated CAF regimen) (n=36) and control group (CAF regimen) (n=34).The activities of T cells and NK cells were examined in two groups before and after treatment,and the toxic, side reaction and living quality were also compared.Results The levels of CD3,CD4,CD8 and the ratio of CD4/CD8 and the activity of NK cells in treatment group were higher than those in control group (P