RESUMO
The haemolymph of Tettigonia cantans (Fuess) has been studied at the preimago and imago stages with both electron and light microscopic methods. PAS-negative granules were detected in histochemical reactions. On electronograms, one type of haemocytes was referred to as granulocytes recognized in haemolymph. In the cytoplasm of granulocytes, two types of granules were found: those of mitochondrial origin, and those being derivatives of the Golgi apparatus. Secret discharge is realized by the merocrine way. Four stages of granulocyte development are distinguished: the formation of granules and organelle development; the formation and accumulation of granules, active secretion, and cell destruction.
Assuntos
Hemócitos/ultraestrutura , Hemolinfa/citologia , Ortópteros/ultraestrutura , Animais , Grânulos Citoplasmáticos/metabolismo , Grânulos Citoplasmáticos/ultraestrutura , Complexo de Golgi/ultraestrutura , Estágios do Ciclo de Vida , Microscopia Eletrônica , Mitocôndrias/ultraestrutura , Ortópteros/fisiologia , Coloração e RotulagemRESUMO
The haemolymph of larvae and imago stages of Decticus verrucirus was studied with electron and light microscope. PAS-positive and PAS-negative granules were detected in haemocytes. On the electronograms, granulocytes were recognized as the only type of haemocytes. In the cytoplasm of granulocytes, granules of two types were found: those of mitochondrial origin, and originating from the Golgi apparatus, respectively. The discharge of a secret is realized by the merocrine way. Four stages of granulocyte development have been distinguished: 1) granule formation and organelle development, 2) granule formation and accumulation, 3) active secretion, and 4) cell destruction.
Assuntos
Hemócitos/ultraestrutura , Hemolinfa/citologia , Ortópteros/ultraestrutura , Animais , Grânulos Citoplasmáticos/ultraestrutura , Complexo de Golgi/ultraestrutura , Estágios do Ciclo de Vida , Microscopia Eletrônica , Ortópteros/fisiologia , Partículas Submitocôndricas/ultraestruturaRESUMO
On the basis of patterns of haemocyte ultrastructure and functions at preimago and imago stages of Metrioptera roeselii, secretory cells of granulocyte type were recognized in the haemolymph. The development of granulocytes was traced starting from their formation up to cell death and destruction. The haemocytes develop as "dark" and "light" cells, differing in their functional activity, although their ultrastructure is similar. In the cytoplasm of granulocytes, granules of both mitochondrial and nuclear origin were detected. Simultaneously two processes occur in the cells--the accumulation and discharge of granules.