Halitosis and Pain Threshold of Peri-Implant Tissues: An Observational Cohort Study.

In this study, we aimed to evaluate the halitosis and pain threshold of the peri-implant soft tissues in individuals rehabilitated with implant-supported prostheses. Forty-eight subjects were divided into four groups (n = 12) according to their prosthetic rehabilitation: single-tooth fixed prosthesis, multi-tooth fixed prosthesis, overdentures, and the Brånemark protocol. Halitosis was measured using a halimeter, whereas the pain threshold was measured using Von Frey monofilaments. Measurements were taken before (t0) and 30 days after (t1) placement of healing caps, and at the time of (t2) and 30 days after (t3) prosthetic placement. Halitosis data were analyzed using the chi-square test and Bonferroni correction (p < 0.05). Two-way ANOVA and Tukey's test (p < 0.05) were used to analyze pain threshold data. We noted an association between halitosis and time for the Brånemark protocol [X2(6) = 18.471; p = 0.005] and overdenture groups [X2(6) = 17.732; p = 0.007], and between halitosis and type of prosthesis only at t0 [X2(6) = 12.894; p = 0.045]. The interaction between time and the type of prosthesis significantly interfered with the mean pain threshold values (p = 0.001). At most time points, the majority of participants in each group had clinically unacceptable halitosis. After 30 days of using the prostheses, the overdenture group had a lower pain threshold compared to the Brånemark protocol group.

Cytotoxicity of cleaning agents for ocular prostheses.

BACKGROUND: Polymethylmethacrylate (PMMA) is the most used material for the manufacturing of eye prostheses. OBJECTIVES: To investigate the cytotoxicity of different cleaning agents for ocular prostheses on human conjunctival cells. MATERIAL AND METHODS: Six groups of specimens were created (saline, soap, 4% chlorhexidine, hydrogen peroxide, 1% triclosan, and citronella oil). Three specimens were made for each disinfectant at each disinfection period (1, 7, 15, 30, 60, and 90 days), totaling 108 specimens. Thus, the specimens were disinfected, with different disinfectants, for different periods of time. After each disinfection process, the specimens were washed with sterile distilled water. A human conjunctival cell line was grown on the acrylic resin specimens and then cytotoxicity tests (MTT and Neutral Red (NR)) were performed. A negative control (untreated cell cultures) and positive control (Tween 20) were created. Two-way analysis of variance (ANOVA) and Bonferroni test were performed (p < 0.05). RESULTS: For the MTT and NR tests, when there was a significant difference between the disinfectant and negative control, the disinfectant generated a significant reduction in cell proliferation most of the time. CONCLUSIONS: All reductions in cell proliferation caused by the disinfectants were clinically acceptable. All disinfectants tested in this study were found to be non-cytotoxic to human conjunctival cells.

Halitosis and Pain Threshold of Peri-Implant Tissues: An Observational Cohort Study

Abstract In this study, we aimed to evaluate the halitosis and pain threshold of the peri-implant soft tissues in individuals rehabilitated with implant-supported prostheses. Forty-eight subjects were divided into four groups (n = 12) according to their prosthetic rehabilitation: single-tooth fixed prosthesis, multi-tooth fixed prosthesis, overdentures, and the Brånemark protocol. Halitosis was measured using a halimeter, whereas the pain threshold was measured using Von Frey monofilaments. Measurements were taken before (t0) and 30 days after (t1) placement of healing caps, and at the time of (t2) and 30 days after (t3) prosthetic placement. Halitosis data were analyzed using the chi-square test and Bonferroni correction (p < 0.05). Two-way ANOVA and Tukey's test (p < 0.05) were used to analyze pain threshold data. We noted an association between halitosis and time for the Brånemark protocol [X2(6) = 18.471; p = 0.005] and overdenture groups [X2(6) = 17.732; p = 0.007], and between halitosis and type of prosthesis only at t0 [X2(6) = 12.894; p = 0.045]. The interaction between time and the type of prosthesis significantly interfered with the mean pain threshold values (p = 0.001). At most time points, the majority of participants in each group had clinically unacceptable halitosis. After 30 days of using the prostheses, the overdenture group had a lower pain threshold compared to the Brånemark protocol group.

Resumo Este estudo teve como objetivo avaliar a halitose e o limiar de dor dos tecidos moles peri-implantares em indivíduos reabilitados com próteses implantossuportadas. Um total de 48 indivíduos foram divididos em quatro grupos (n=12), de acordo com as reabilitações: prótese fixa unitária, prótese fixa multidentária, sobredentadura e protocolo de Brånemark. A halitose foi medida com um halímetro, enquanto o limiar de dor foi medido com monofilamentos de von Frey. As medições foram feitas antes (t0) e 30 dias após (t1) a colocação das tampas de cicatrização e no momento (t2) e 30 dias após (t3) a colocação da prótese. Os dados de halitose foram analisados por meio do teste qui-quadrado e correção de Bonferroni (p < 0,05). ANOVA de duas vias e o teste de Tukey (p < 0,05) foram usados para analisar os dados do limiar de dor. Observou-se associação entre halitose e tempo para o protocolo de Brånemark [X2(6) = 18,471; p = 0,005] e grupos overdenture [X2(6) = 17,732; p = 0,007], e entre halitose e tipo de prótese apenas em t0 [X2(6) = 12,894; p = 0,045]. A interação entre o tempo e o tipo de prótese interferiu significativamente nos valores médios do limiar de dor (p = 0,001). Na maioria dos pontos de tempos, a maioria dos participantes de cada grupo apresentava halitose clinicamente inaceitável. Após 30 dias de uso das próteses, o grupo overdenture apresentou menor limiar de dor em comparação ao grupo do protocolo de Brånemark.

Effect of glaze and chlorhexidine on physical and mechanical properties of bis-acryl resin: An in situ study.

BACKGROUND: Temporary prosthesis protects the oral tissues, in addition to providing aesthetic look and masticatory function until a definitive prosthesis is manufactured. OBJECTIVES: To evaluate the effect of glaze and 0.12% chlorhexidine (CHX) on the physical and mechanical properties of bis-acryl, and to evaluate the antimicrobial efficacy of CHX. MATERIAL AND METHODS: Eighty specimens of bis-acryl resin were made. Over 40 of them the glaze was applied. One specimen with and 1 specimen without glaze were placed in niches of an appliance manufactured for each patient. Each of the 20 volunteers received 2 devices. Initially, the volunteers used one device and treated it with sucrose for 7 days (control), and later they used the other device and treated it with sucrose and CHX for 7 days (test). Color, microhardness, roughness, surface energy, and insoluble extracellular polysaccharides (EPS) tests were performed. All results were submitted to the Tukey's test, with the exception of the EPS results, which were submitted to the Student's t test. RESULTS: The ΔE00 of the unglazed control group was significantly higher than that of the unglazed test group. In all groups, a significant decrease in microhardness occurred over time. At both times, the glaze significantly increased the microhardness of the specimens (in all the glazed groups). At the final time, the test glaze group showed significantly higher microhardness compared with the control glaze group. Roughness in the groups without glaze increased significantly with CHX treatment over time. At both times, the glaze generated a significant reduction in roughness in the control and test groups. There was a significant reduction in surface energy over time in all groups. In most comparisons, the glazed groups showed significantly higher surface energy values compared with the unglazed control group. At the final time point, the unglazed test group showed a significantly higher surface energy value than the unglazed control group; and the glazed test group showed a significantly higher surface energy value compared with the glazed control group. The resins that received CHX had a significantly lower amount of biofilm. CONCLUSIONS: Color values were clinically acceptable in all tested groups. At both time points, the roughness values were clinically acceptable only in the glazed groups. Glaze increased the microhardness of the specimens. Microhardness and surface energy were reduced over time in all groups. Chlorhexidine can help prevent microhardness degradation. Glaze and CHX can increase surface energy. Chlorhexidine reduced the amount of bacterial biofilm.

Extended-pour and conventional alginates: effect of storage time on dimensional accuracy and maintenance of details.

OBJECTIVE: This study aimed to evaluate the dimensional stability and maintenance of details of conventional and high stability alginates up to 5-day storage. METHODS: Two types of alginates were selected (n=10) for this study, conventional (Hydrogum) and high stability alginates (Hydrogum 5), which were produced with the aid of a cylindrical metal block and a ring-shaped metal mold (Specifications 18, 19, and 25, ANSI/ADA). Ten images were obtained from the molds for the dimensional stability test, which were taken immediately after their production and at each different storage periods (15 min, 24 h, 48 h, 72 h, 96 h, and 120 h) by a digital camera. The specimens were kept hermetically sealed in plastic bags (23°C) and then used to obtain 140 (n=70) dental stone models, used in the detail reproduction test, in which the angular accuracy of three grooves (20 µm, 50 µm, and 75 µm) was observed at each period. The details reproduction accuracy was classified using a predetermined score classification. Measurements of dimensional changes were made in the Corel DRAW X6 program. The data were submitted to the Student's t-test (α?#8197;= 0.05). RESULTS: A statistically significant difference concerning the size of the matrix was observed after 24h for both alginates, and a statistically significant negative linear dimensional change (contraction) was verified after 24 h of storage (1.52% for the high stability alginate, and 1.32% for the conventional alginate). The high stability alginate kept the full details for 72 hours, while the conventional alginate, for 24 h. Both alginates reproduced the 75 µm groove at all storage periods. CONCLUSION: Impressions made with both alginates presented satisfactory clinical results when the alginates were immediately poured.

Avaliação dos tratamentos de superfície na resistência de união entre dois pinos de fibra de vidro e uma resina composta / Evaluation of surface treatments on the bond strength between two fiberglass posts and a composite resin

Introdução: O objetivo deste trabalho foi avaliar a influência de diferentes métodos de tratamento de superfície em pinos de fibra de vidro com relação a sua força de união com a resina composta e avaliar os tipos de falhas ocorridas. Materiais e Métodos: Foram utilizados 36 pinos da WhitepostDC (grupo GF) e Exacto Cônicos (grupo GA), divididos em: Grupos controle GF-1 e GA-1 ­ aplicação de silano de acordo com as especificações do fabricante; Grupos GF-2 e GA-2 ­ condicionamento com peróxido de hidrogênio a 10% e silanização; Grupos GF-3 e GA-3 ­ Jateamento com óxido de alumínio por 10 segundos e silanização. A adição da resina composta foi realizada em incrementos de 2 mm, com fotopolimerização de cada incremento por 40 segundos, até o preenchimento de toda extensão da matriz. Então, o conjunto pino e resina composta foi seccionado em três porções (cervical, médio e apical) (n = 18). As amostras foram submetidas à análise de resistência de união através do teste Push-Out em uma Máquina de Ensaio Universal (EMIC), e os tipos de falhas foram avaliados com aumento 30 × com um microscópio óptico. Os dados foram submetidos à análise de variância, ao teste de Tukey e ao teste de qui-quadrado com significância de 5%. Resultados: Nos grupos GA, o tratamento com silano (23,10 MPa) resultou em resistência de união estatisticamente maior que os tratamentos com peróxido (17,19 MPa) e com jateamento (16,28 MPa). No tratamento com peróxido, a resistência de união foi estatisticamente maior no terço médio (21,16 MPa) que no terço apical (12,55 MPa). A falha do tipo mista teve a maior prevalência (57,01%). Conclusão: O tratamento com silano apresentou maior resistência de união, com diferença estatística dos demais grupos na marca Angelus

Introduction: The aim of this study was to evaluate the influence of different surface treatment methods on glass-fiber posts relative to their bond strength with the composite resin and to evaluate the types of failures that occurred. Materials and Methods: Thirty-six posts from WhitepostDC (FG group) and Exacto Conics (AG group) were used, divided into: Control FG-1 and AG-1 groups ­ application of silane, according to manufacturers' specifications; FG-2 and AG-2 groups ­ conditioning with 10% hydrogen peroxide and silanization; FG-3 and AG-3 groups ­ aluminum oxide blasting for 10 seconds and silanization. The composite resin was added in 2-mm increments, each increment was photopolymerized for 40 seconds, until the entire length of the matrix was filled. Then, the assembly (fiber post and composite resin) was sectioned into three portions (cervical, middle and apical) (n = 18). Samples were submitted to the bond strength analysis by a push-out test in a Universal Testing Machine and the failure types were evaluated with a 30 × magnification with an optical microscope. Data were submitted to the analysis of variance, the Tukey test, and the chi-square test with a 5% significance level. Results: For AG groups, the treatment with silane (23.10 MPa) resulted in statistically greater bond strength than the treatments with peroxide (17.19 MPa) and blasting (16.28 MPa). It was found that the bond strength was statistically higher in the middle third (21.16 MPa) than in the apical third (12.55 MPa) for the peroxide treatment. The mixed-type failure had the highest prevalence (57.01%).

Extended-pour and conventional alginates: effect of storage time on dimensional accuracy and maintenance of details

ABSTRACT Objective: This study aimed to evaluate the dimensional stability and maintenance of details of conventional and high stability alginates up to 5-day storage. Methods: Two types of alginates were selected (n=10) for this study, conventional (Hydrogum) and high stability alginates (Hydrogum 5), which were produced with the aid of a cylindrical metal block and a ring-shaped metal mold (Specifications 18, 19, and 25, ANSI/ADA). Ten images were obtained from the molds for the dimensional stability test, which were taken immediately after their production and at each different storage periods (15 min, 24 h, 48 h, 72 h, 96 h, and 120 h) by a digital camera. The specimens were kept hermetically sealed in plastic bags (23°C) and then used to obtain 140 (n=70) dental stone models, used in the detail reproduction test, in which the angular accuracy of three grooves (20 µm, 50 µm, and 75 µm) was observed at each period. The details reproduction accuracy was classified using a predetermined score classification. Measurements of dimensional changes were made in the Corel DRAW X6 program. The data were submitted to the Student's t-test (α?#8197;= 0.05). Results: A statistically significant difference concerning the size of the matrix was observed after 24h for both alginates, and a statistically significant negative linear dimensional change (contraction) was verified after 24 h of storage (1.52% for the high stability alginate, and 1.32% for the conventional alginate). The high stability alginate kept the full details for 72 hours, while the conventional alginate, for 24 h. Both alginates reproduced the 75 µm groove at all storage periods. Conclusion: Impressions made with both alginates presented satisfactory clinical results when the alginates were immediately poured.

RESUMO Objetivo: O presente estudo teve como objetivo avaliar a estabilidade dimensional e manutenção de detalhes de alginatos convencionais e de alta estabilidade por até 5 dias de armazenamento. Métodos: Para esse estudo, foram selecionados dois tipos de alginatos: convencional (Hydrogum) (n = 10) e de alta estabilidade (Hydrogum 5) (n = 10), sendo produzidos com o auxílio de um bloco metálico cilíndrico e um molde metálico em forma de anel (especificações 18, 19 e 25, ANSI/ADA). Para o teste de estabilidade dimensional, dez imagens foram obtidas imediatamente e para cada tempo de armazenamento (15 min, 24, 48, 72, 96 e 120 horas), realizadas por câmera digital. As amostras foram mantidas hermeticamente fechadas em sacos plásticos (23°C). Para o teste de reprodução de detalhes, as amostras foram utilizadas para obter 140 (n = 70 por grupo) modelos de gesso, sendo observada a precisão angular de três sulcos (20 µm, 50 µm e 75 µm) para cada período. A precisão da reprodução de detalhes foi classificada usando uma classificação de pontuação predeterminada. As medições das mudanças dimensionais foram feitas no programa Corel DRAW X6. Os dados foram submetidos ao teste t de Student (α = 0,05). Resultados: Foi observada diferença estatística em relação à matriz após 24h para ambos os alginatos. Uma mudança dimensional linear negativa estatisticamente significativa (contração) foi verificada após 24 h de armazenamento (1,52% para alginato de alta estabilidade; 1,32% para alginato convencional). O alginato de alta estabilidade manteve os detalhes completos por até 72 horas, enquanto o alginato convencional, por 24 horas. Os alginatos reproduziram o sulco de 75 µm para todos os períodos. Conclusão: As impressões feitas com ambos os alginatos devem ser imediatamente vazadas para se ter resultados clínicos satisfatórios.

Prevalence of symptoms of temporomandibular disorders, oral behaviors, anxiety, and depression in Dentistry students during the period of social isolation due to COVID-19.

OBJECTIVE: Temporomandibular dysfunction (TMD), anxiety, and depression are disorders that, due to the current lifestyle, are affecting an increasing portion of the population. Investigating the prevalence of the symptoms of these disorders during the quarantine due to the coronavirus 2019 pandemic (COVID-19) is important to outline clinical strategies for patient care. This study assessed the prevalence of TMD symptoms, anxiety, depression, and oral behaviors and their associations during the social isolation due to COVID-19. METHODOLOGY: Questionnaires were used to assess TMD symptoms in accordance with the Diagnostic Criteria for Temporomandibular Disorders: clinical protocol and assessment instruments, a questionnaire to verify oral behaviors and Hospital Anxiety and Depression Scale to assess symptoms of anxiety and depression in students of dentistry at the Faculty of Health Sciences of the University of Brasília in May 2020. Qualitative data were subjected to descriptive statistics and chi-squared analysis (p<0.05). The relationship between quantitative and qualitative data was evaluated using Spearman's rho correlation (p<0.05). RESULTS: There was a high prevalence of TMD symptoms, anxiety, and depression in the participants, resulting in association between gender and anxiety symptoms (p=0.029). There was a positive correlation between oral behaviors and TMD symptoms (r=0.364; p<0.001), between oral behaviors and anxiety symptoms (r=0.312; p=0.001), and between oral behaviors and symptoms of depression (r=0.216; p=0.021). CONCLUSION: Social isolation due to the COVID-19 pandemic has an impact on the prevalence of TMD symptoms, anxiety, and depression.

In vitro evaluation of microbial adhesion on the different surface roughness of acrylic resin specific for ocular prosthesis.

OBJECTIVE: The purpose of this study was to evaluate the influence of surface roughness in biofilm formation of four microorganisms (Staphylococcus epidermidis, Staphylococcus aureus, Enterococcus faecalis, and Candida albicans) on acrylic resin surface of ocular prostheses. MATERIALS AND METHODS: Acrylic resin samples were divided into six groups according to polishing: Group 1200S (1200 grit + silica solution); Group 1200; Group 800; Group 400; Group 120 and Group unpolished. Surface roughness was measured using a profilometer and surface images obtained with atomic force microscopy. Microbial growth was evaluated after 4, 24, and 48 hours of incubation by counting colony-forming units. STATISTICAL ANALYSIS USED: For roughness, it was performed 1-way ANOVA and parametric Tukey test α5% (P ≤ 0.05). For CFU data found, it was applied Kruskal-Wallis and Mann-Whitney tests. RESULTS: Group 120 and 400 presented the highest roughness values. For S. epidermidis and S. aureus, Group 1200S presented the lowest values of microbial growth. For E. faecalis at 4 hour, microbial growth was not observed. C. albicans did not adhere to the acrylic resin. Except for Group 1200S, different surface roughnesses did not statistically interfere with microbial adhesion and growth on acrylic surfaces of ocular prostheses. CONCLUSIONS: The roughness did not interfere with the microbial adhesion of the microorganisms evaluated. The use of silica decreases significantly microbial growth.

Reabilitação estética e funcional em paciente com desgaste dental acentuado: relato de caso clínico / Esthetic and functional rehabilitation in a patient with excessive dental wear: a case report

O uso de facetas cerâmicas de dissilicato de lítio é uma excelente opção para a correção estética e funcional de dentes com alteração de formato, como o causado por desgaste dental acentuado, pois esse material apresenta características mecânicas e ópticas adequadas. Assim, o objetivo deste estudo foi descrever os procedimentos de reabilitação com facetas cerâmicas de um paciente jovem com desgaste dental acentuado. Paciente do gênero masculino, 26 anos, buscou atendimento devido à cor insatisfatória dos seus dentes. Durante a anamnese, paciente relatou não ranger ou apertar os dentes, contudo afirmou elevado consumo de alimentos ácidos e uso de força excessiva durante a escovação dental. Durante o exame clínico, foi observado desgaste dental acentuado na face vestibular dos dentes maxilares e leve na superfície oclusal dos dentes posteriores. Foi proposta a instalação de facetas cerâmicas de dissilicato de lítio dos elementos 15 ao 25 e de placa interceptora. O resultado da reabilitação foi satisfatório para o paciente devido ao reestabelecimento da estética, função e auto-estima(AU)

The use of ceramic facets of lithium disilicate is an excellent option for the aesthetic and functional correction of teeth with alteration of shape, such as that caused by accentuated dental wear, since this material presents adequate mechanical and optical characteristics. Thus, the objective of this study was to describe the rehabilitation procedures with ceramic facets of a young patient with marked dental wear. A male patient, 26 years old, sought care due to the unsatisfactory color of his teeth. During the anamnesis, patient reported no grinding or grinding of teeth, however, he stated high consumption of acidic foods and excessive force during dental brushing. During the clinical examination, sharp dental wear was observed on the buccal face of the maxillary teeth and light on the occlusal surface of the posterior teeth. It was proposed the installation of ceramic facets of lithium disilicate of elements 15 to 25 and of interceptor plate. The result of the rehabilitation was satisfactory for the patient due to the reestablishment of aesthetics, function and self-esteem(AU)

Effect of nonthermal plasma on the properties of a resinous liner submitted to aging.

STATEMENT OF PROBLEM: The properties, such as softness and viscoelasticity, of a resinous reliner can deteriorate and extrinsic elements can become incorporated, making surface protection of the reliner material essential. PURPOSE: The purpose of this in vitro study was to evaluate the effect of low temperature plasma on Coe-Soft resinous reliner, submitted to aging in artificial saliva for up to 180 days. Sorption, solubility, Shore A hardness, surface energy, and topographic characteristics were analyzed by scanning electronic microscopy (SEM) and energy-dispersive spectroscopy (EDS). MATERIAL AND METHODS: Forty-four specimens were fabricated and distributed in 2 groups: nonplasma reliner (control group) and reliner with plasma (plasma group). The plasma was applied with a mixture of 70% hexamethyldisiloxane, 20% O, and 10% Ar. Total work pressure was maintained at a constant 20 Pa for 30 minutes of deposition. The specimens were analyzed before and after aging in an incubator with immersion in artificial saliva for 30, 90, and 180 days. The quantitative data were submitted to 2-way ANOVA and the Tukey test (α=.05), while qualitative data were compared visually. RESULTS: The control group presented lower Shore A hardness values only in the initial period, and surface energy increased with aging for both groups until 90 days. Greater sorption percentage values were encountered at 180 days in the plasma group. Greater solubility values were encountered in the control group in all periods. CONCLUSIONS: Plasma is an option for the protection of the material studied because the deposited film remained on the surface of the reliner material after aging.

Biocompatibilidade da resina acrílica de prótese ocular: análise in vitro e in vivo / Biocompatibility of ocular prosthesis acrylic resin: in vitro and in vivo analysis

O objetivo desse estudo foi avaliar a influência de diferentes ciclos e métodos de polimerização da resina acrílica (RA) branca de próteses oculares sobre a biocompatibilidade de células da conjuntiva humana e resposta inflamatória do tecido subcutâneo de ratos. Para isso, foram confeccionados corpos de prova em RA termopolimerizados em água aquecida (RNAA), por energia de microondas (RNTM) e quimicamente ativados (RNQA). Para a análise in vivo, a resposta inflamatória desses 3 grupos (n=20/grupo) foi avaliada no tecido subcutâneo de 20 ratos Wistar por 7, 15, 30 e 60 dias (d). Células inflamatórias foram contadas no tecido adjacente ao corpo de prova após coloração com hematoxilina e eosina. A análise imunohistoquímica foi realizada para a detecção de IL-1ß, IL-6, TNFα, IL-17 e CCL20. Para a análise in vitro, diferentes ciclos de polimerização para cada método citado foram avaliados, totalizando 11 grupos (n=8/grupo). Foram realizadas análises de grau de conversão (GC), MTT, Alamar Blue, ELISA, RT-PCR em tempo real e dupla marcação de Anexina V e iodeto de propídio. Dados quantitativos foram submetidos à Análise de Variância e ao teste de Tukey com significância de 5%. Os resultados qualitativos foram comparados visualmente. Na análise in vivo, houve infiltrado inflamatório moderado para os grupos RNTM e RNQA e leve para o grupo RNAA após 7 d. O infiltrado inflamatório e a imunomarcação dos alvos testados diminuiu gradativamente ao longo dos 60 d. O grupo RNTM exibiu mais células inflamatórias, com exceção do grupo RNAA, que apresentou mais eosinófilos e linfócitos após 15 d, e do grupo RNQA, onde foi observado mais macrófagos em 15 d e neutrófilos em 60 d. Os grupos RNAA e RNQA apresentaram maior imunomarcação de IL-1ß após 7 d. O grupo RNQA apresentou maior imunomarcação de IL-1ß (15 e 30 d), IL-6 (30 e 60 d), IL-17 (15 e 30 d) e TNF-α (7 d). Os grupos RNAA e RNTM apresentaram maior imunomarcação de TNF-α nos períodos de 15 e 30 d, enquanto o grupo RNTM, aos 60 d. Na análise in vitro, todos os grupos apresentaram proliferação celular maior que 75%. O ciclo longo de polimerização em microondas apresentou menor GC e percentual de proliferação celular no MTT e resultou em grande liberação de IL-2. No ensaio de Alamar Blue, esse grupo apresentou baixo percentual de proliferação celular, assim como o grupo que recebeu ciclo longo de polimerização em água aquecida e grupos submetidos à ativação química. Maior liberação de IL-6 foi observada nos grupos submetidos à ativação química e de IL-23 para o ciclo curto de polimerização em microondas. Maior expressão gênica de TGF ß1 ocorreu para o grupo que recebeu ciclo longo de polimerização em água aquecida seguido de 30 min de armazenamento em água. Maior expressão gênica de CASP9 ocorreu para o grupo ativado quimicamente sobre a bancada. Pode-se concluir que os métodos de polimerização por meio de energia de microondas (ciclos longo e curto) e por ativação química desencadearam uma resposta inflamatória mais intensa. Dentre os métodos de polimerização recomendados pelo fabricante, a polimerização em água aquecida apresentou resultados mais satisfatórios(AU)

The aim of this study was to evaluate the influence of different cycles and methods of white color acrylic resin (AR) for ocular prosthesis on the biocompatibility of human conjunctival cells and on the inflammatory response of rat subcutaneous tissue. For this, AR specimens were prepared in water bath (NRWB), by microwave energy (NRME), and chemically activated (ANR). For in vivo analysis, the inflammatory response of these 3 groups (n=20/group) was assessed in subcutaneous tissue of 20 Wistar rats at 7, 15, 30 and 60 days (d). Inflammatory cells were counted in the tissue adjacent to specimen after staining with hematoxylin and eosin. The immunohistochemical analysis was performed for the detection of IL-1ß, IL-6, TNFα, IL-17, and CCL20. For in vitro analysis, different cycles of polymerization for each method were evaluated, with a total of 11 groups (n=8/group). The degree of conversion (DC), MTT, ELISA, real-time RT-PCR and Annexin V and propidium iodide assays were performed. Quantitative data were submitted to Analysis of Variance and Tukey test with a 5% significance. Qualitative data were submitted to visual comparison. In in vivo analysis, there was a moderate inflammatory infiltrate for groups NRME and ANR, and a light infiltrate for the group NRWB after 7 d. The inflammatory infiltrate and the immunolabeling of tested targets decreased gradually during the 60 d. The group NRME exhibited the highest number of inflammatory cells, except for the group NRWB, which presented a higher number of eosinophils and lymphocytes after 15 d, and for the group ANR, where a higher number of macrophages and neutrophils were observed at 15 d and at 60 d, respectively. Groups NRWB and ANR showed higher IL-1ß immunolabeling after 7 d. The group ANR had the highest immunolabeling of IL-1ß (15 and 30 d), IL-6 (30 and 60 d), IL-17 (15 and 30 d), and TNF-α (7 d). Groups NRWB and NRME showed greater immunolabeling in the periods of 15 and 30 d, while the group NRME had also high results at 60 d. In in vitro analysis, all groups showed cell proliferation higher than 75%. The long cycle of polymerization using microwave energy resulted in lower DC and lower percentage of cell proliferation in the MTT assay and in large release of IL-2. In the Alamar Blue assay, this group had a low percentage of cell proliferation, as well as the group that received a long cycle of polymerization in water bath and groups submitted to chemical activation. A higher release of IL-6 was observed in groups submitted to chemical activation and of IL-23, for the short cycle of polymerization in microwave. Higher TGF ß1 gene expression occurred for the group that received long cycle of polymerization in water bath followed by 30 min of storage in water. Higher CASP 9 gene expression occurred for the chemically activated group on bench. It can be concluded that the polymerization by microwave energy (long and short cycles) and by chemical activation resulted in higher inflammatory response. Among methods recommended by the manufacturer, the water bath polymerization showed more satisfactory results(AU)

Instalação de prótese provisória implantossuportada: relato de caso / Installation of implant-supported provisional prosthesis: case report

A busca por restaurações protéticas suportadas por implantes está aumentando cada vez mais na odontologia, e é uma parte importante do tratamento reabilitador, principalmente em regiões estéticas. Desta forma, o objetivo deste estudo foi descrever o caso clínico de um paciente reabilitado com implante e posterior instalação de prótese provisória em região anterior de maxila, reestabelecendo sua estética, fonética e função. Paciente do sexo masculino procurou atendimento devido à fratura do elemento 2.2. Ao exame clínico, foi observada raiz residual desse elemento. Então, foi proposto ao paciente a exodontia da raiz residual, instalação de implante imediato e posterior instalação de prótese provisória. Após seis meses de acompanhamento, o paciente encontra-se satisfeito com a aparência clínica dos tecidos periodontais adjacentes, sendo então iniciado o tratamento reabilitador protético com a prótese definitiva(AU)

The search for implant-supported prosthetic restorations is increasing in dentistry, and is an important topic of the rehabilitation treatment, especially in esthetic regions. Thus, the aim of this study was to describe a clinical case of a patient rehabilitated with implant and later with provisional prosthesis in the maxilla anterior region, reestablishing esthetics, phonetics and function. A male patient sought the Department of Dental Materials and Prosthodontics of the Faculty of Dentistry of Aracatuba/UNESP due to a fracture of the left lateral incisor. During the clinical exam, a residual tooth root was observed. Then, the extraction of the residual root was proposed, followed by the installation of an immediate implant and subsequent installation of provisional prosthesis. After six months of follow-up, the patient was satisfied with the clinical appearance of the adjacent periodontal tissues and started the prosthetic treatment with the definitive prosthesis(AU)

Push-out bond strength of glass fiber posts luted with two resin cements / Resistência à tração de pinos de fibra de vidro cimentados com dois tipos de cimento resinoso

Objectives: To evaluate the bond strength of a prefabricated glass fiber post to the root dentin with two resin cements using the push-out test. Material and Methods: Thirty fiber posts (White Post ECD) were cemented in human endodontically treated anterior teeth with two resin cements (n=15): RelyxTM ARC and AllCem. The roots were transversely sectioned and divided in cervical, middle and apical thirds after 48 h of cementation. The specimens' retention was tested by push-out method. The results were analyzed by 2-way ANOVA and Tukey's test (p <0.05). Results: There were no significant differences in the bond strength between the resin cements. The bond strength was higher in cervical third than in middle and apical thirds for both resin cements. It was observed higher percentages of adhesive failures followed by mixed. Conclusions: Both resin cements are indicated in the cementation of glass fiber post. (AU)

Objetivo: Avaliar a resistência de união entre dentina radicular e pino pré-fabricado, utilizando dois sistemas cimentantes, por meio do teste push-out. Material e Métodos: Trinta pinos de fibra de vidro (White Post DCE) foram cimentados a dentes humanos anteriores, tratados endodonticamente, com o auxílio de dois sistemas cimentantes (n=15): RelyxTM ARC e AllCem. Após 48 horas da cimentação, as raízes foram seccionadas transversalmente e divididas em terços cervical, médio e apical. Os corpos-de-prova foram submetidos ao teste de cisalhamento por extrusão "push-out". Os resultados foram submetidos aos testes estatísticos de análise de variância (2-way ANOVA) e de Tukey y (p<0,05). Resultados: Não houve diferença estatisticamente significativa na resistência de união entre os dois cimentos resinosos utilizados. A resistência de união foi maior no terço cervical do que nos terços médio e apical. Foi observada maior frequência de falhas adesivas seguida das mistas. Conclusão: Os dois cimentos resinosos são sugeridos para a cimentação de pinos de fibra de vidro (AU)

Planejamento e instalação de restaurações cerâmicas: relato de caso / Planning and restoration ceramic installation: case report

Nos dias atuais, a cerâmica tem se tornado o material de escolha nas reabilitações estéticas, apresentando muitas vantagens em relação à resina composta. Portando, é de grande importância que o profissional tenha conhecimento sobre sua correta indicação, sabendo avaliar adequadamente fatores como substrato dental, tecidos gengivais, condições endodônticas e anseios do paciente, e saiba aplicar a técnica de forma correta, garantindo o sucesso clínico e longevidade da restauração. Desta forma o objetivo deste estudo foi descrever a técnica operatória de substituição de laminados cerâmicos para a reabilitação estética e funcional do sorriso, bem como suas indicações

Nowadays, the pottery has become the material of choice in aesthetic restorations, with many advantages over the composite. Porting is of great importance that the trader is aware of the correct indication, knowing properly assess factors such as dental substrate, gingival tissues, endodontic conditions and wishes of the patient, and learn to apply the technique correctly, ensuring the clinical success and longevity restoration. Thus the aim of this study was to describe the surgical technique of substitution of ceramic laminates for the aesthetic and functional rehabilitation of the smile, and his instructions

Pigmentação de restaurações de resina composta: uma revisão de literatura / Color alteration of composite resins: a literature review

As resinas compostas são materiais restauradores que reproduzem as características dos tecidos dentais. Contudo, frente aos desafios mecânicos e químicos a que são submetidas na cavidade bucal, sofrem efeitos indesejáveis, como pigmentação e manchamento. O objetivo do presente estudo foi revisar a literatura sobre a etiologia da pigmentação da resina composta e expor alternativas clínicas e domiciliares para reduzir os seus efeitos na alteração de cor deste material odontológico estético. Foi realizado um levantamento bibliográfico na base de dados do Pubmed, no período de agosto de 2009 à agosto de 2014 (5 anos). Os termos utilizados foram: “composite resin and color stability”, “composite resin and pigmentation” e “composite resin and color and pigmentation”. Os critérios de inclusão foram estudos clínicos em humanos e estudos in vitro, na língua inglesa, que avaliaram a estabilidade de cor da resina composta direta e indireta. A pigmentação pode ser atribuída a fatores intrínsecos e extrínsecos. Os fatores intrínsecos se referem à descoloração oriunda de componentes do próprio material, enquanto os fatores extrínsecos estão associados aos hábitos e à dieta do individuo, como o consumo de bebidas e alimentos que possuem corante em sua composição. O profissional pode intervir na prevenção ou redução desses manchamentos através de adequada técnica de confecção das restaurações (polimerização, acabamento e polimento adequados) e, na orientação dos pacientes com relação à higiene oral e aos hábitos que interfiram diretamente na estabilidade de cor do material

Composite resins are restorative materials that reproduce dental tissue characteristics. However, this material is constantly exposed to chemical and mechanical challenges in the oral cavity, resulting in pigmentation and staining. The aim of this study was to review the literature regarding the composite resin pigmentation etiology and to propose clinical and home alternatives to prevent this undesirable characteristic. A literature review was conducted in Pubmed database, from August 2009 to August 2014 (5 years). The terms used were: “composite resin and color stability”, “composite resin and pigmentation” and “composite resin and color and pigmentation”. The inclusion criteria were clinical and in vitro studies, in English, that evaluated the color stability of direct and indirect composite resins. The pigmentation can be attributed to intrinsic and extrinsic factors. Intrinsic factors refer to discoloration resultant from components of the material itself, while extrinsic factors are associated to the diet and habits, as the consumption of beverages and foods, which have pigment composition. The professional can intervene in preventing or reducing the pigmentation through proper technique of restorations (proper polymerization, finishing and polishing) and in guiding patients regarding oral hygiene and habits that directly interfere on material color stability

A influência da radioterapia e da quimioterapia sobre próteses obturadoras retidas por implante: revisão de literatura / The influence of radiotherapy and chemotherapy on obturator prosthesis retained by implants: literature review

A prótese obturadora é uma alternativa para a reabilitação funcional e estética de pacientes que se submeteram a ressecção cirúrgica total ou parcial da maxila. Para a obtenção de maior estabilidade e retenção desta prótese, faz-se necessário a associação de implantes osseointegrados para a maioria dos pacientes. Porém, quando se trata de pacientes com história de câncer de cabeça e pescoço, ainda não há consenso na literatura quanto a essa associação, devido ao envolvimento da radioterapia e/ou quimioterapia durante o tratamento, as quais possuem efeitos secundários como: xerostomia, diminuição do suprimento vascular, dificuldade na abertura de boca e deglutição, e a dificuldade em tolerar a prótese. Contudo, a oxigenoterapia hiperbárica tem sido utilizada como um recurso para melhorar a osseointegração dos implantes, aumentando a oxigenação dos tecidos irradiados. Pode-se verificar que a associação da prótese obturadora com implantes osseointegrados, conjuntamente com a radioterapia e/ou quimioterapia, resultaram em melhora na qualidade de vida da maioria dos pacientes estudados

The obturator prosthesis is an alternative for functional and esthetic rehabilitation of patients who have been submitted total or partial surgical resection of the jaw. To obtain increased stability and retention of this prosthesis, osseointegrated implants are due for most patients. However, when dealing with patients with head and neck cancer history, there is no consensus in the literature about this association due to the involvement of radiotherapy and / or chemotherapy during treatment, which have side effects such as: dry mouth, decreased vascular supply, difficulty of mouth opening and swallowing, and difficulty tolerating the prosthesis. Although, the hyperbaric oxygen therapy has been used as a resource to improve osseointegration of the implants, increasing the oxygenation of the irradiated tissues. It was shown that the combination of obturator prosthesis with osseointegrated implants in conjunction with radiotherapy and / or chemotherapy, resulted in improved quality of life for most patients

Análise in vitro da proliferação celular e produção de mediadores inflamatórios por células da conjuntiva estimuladas por materiais utilizados na confecção de próteses oculares / In vitro analysis of cell proliferation and the production of inflammatory mediators by conjunctiva cells stimulated by ocular prosthesis materials

A prótese ocular é utilizada para a reabilitação estética e funcional da ausência ocular. O conhecimento da sua biocompatibilidade é importante para a utilização sem reações danosas aos usuários. O objetivo neste estudo foi avaliar a citotoxicidade de materiais utilizados na confecção de próteses oculares, por meio da análise da proliferação celular e da produção de citocinas pró-inflamatórias e de proteínas de matriz extracelular por células da conjuntiva humana. Inicialmente, foi analisada a influência de diferentes períodos de formação e de exposição dos extratos de resina acrílica branca (N1), termopolimerizada em água aquecida, sobre culturas de células da conjuntiva. Foram confeccionados 24 corpos de prova em resina, sendo 12 para cada período de exposição de células da conjuntiva aos extratos da resina testada (24 e 72 horas). Após a formação dos extratos por 24, 48 e 72 horas de imersão em meio de cultura e, 24 horas em água seguido de 24 horas de imersão em meio, os ensaios propostos foram realizados (n=3). Em seguida, foi avaliado o efeito citotóxico de diferentes métodos de polimerização de resina acrílica N1 em células da conjuntiva. Foram confeccionados 9 corpos de prova em resina (n=3), termopolimerizados em água aquecida, por energia de microondas ou ativados quimicamente, utilizados para a formação dos extratos dessas resinas. Os extratos foram obtidos após 72 horas de imersão dos corpos de prova em meio de cultura e, então, expostos às células da conjuntiva por 72 horas para a realização dos ensaios propostos. Adicionalmente, foi analisada a influência da presença do pigmento acrílico na confecção da prótese de resina acrílica N1, termopolimerizada em água aquecida. Foram confeccionados 9 corpos de prova (n=3): resina N1, resina N1 + pigmento e, pigmento, utilizados para a formação dos extratos desses materiais. Os extratos formaram-se por 72 horas de imersão dos corpos de prova em meio de cultura e, então, foram expostos às células da...

Ocular prosthesis is a treatment option for esthetical and functional rehabilitation of ocular absence. The knowledge of ocular prosthesis material’s biocompatibility is important to ensure a safe use in patients. The aim of this study was to evaluate the cytotoxic effect of ocular prosthesis materials, through the analysis of the cell proliferation, and the production of proinflammatory cytokines and extracellular matrix proteins by a human conjunctival cell line. Initially, the influence of different preparation and exposition periods of eluates from heat-polymerized ocular prosthesis N1 color acrylic resin in human conjunctival cell line was evaluated. A total of 24 acrylic resin samples were manufactured and divided into 2 groups, according to the eluate exposition period to conjunctival cell line (24 and 72 hours). Eluates corresponding to 24, 48 and 72 hours of resin sample immersion in medium and, 24 hours of resin sample immersion in water followed by 24 hours of immersion in medium, were prepared (n=3) for the proposed tests. Then, the cytotoxic effect of different polymerization methods of ocular prosthesis N1 color acrylic resin was analyzed. A total of 9 acrylic resin samples were manufactured (n=3), according to the polymerization method: heat-polymerization in water bath, polymerization by microwave energy and auto-polymerization. Eluates corresponding to 72 hours of resin sample immersion in medium were prepared for proposed tests and exposed to conjunctival cell line for 72 hours. Additionally, the influence of pigment incorporation on the cytotoxicity of heat-polymerized ocular prosthesis N1 color acrylic resin was evaluated. A total of 9 samples were manufactured (n=3): N1 color acrylic resin without pigment incorporation, N1 color acrylic resin with pigment incorporation, and acrylic pigment. Eluates corresponding to 72 hours of sample immersion in medium were prepared and exposed to conjunctival cell line for 72 hours. The cytotoxic effect...

Effect of the inclusion procedure on the alteration of teeth position in maxillary complete dentures / Efeito dos procedimentos de inclusão na alteração da posição dos dentes em dentaduras completas maxilares

As alterações dimensionais das próteses podem comprometer a adaptação e retenção associadas à alteração oclusal, que são considerados fatores relevantes para a confecção de prótese total. Objetivo: O objetivo deste estudo foi o de avaliar o deslocamento linear dos dentes durante o processamento de próteses totais superiores submetidas a dois métodos de inclusão e polimerização convencional. Material e Métodos: Vinte próteses totais superiores foram fabricadas: G1- inclusão com barreira de silicone, e G2 inclusão com barreira de gesso tipo IV. Pontos foram marcados nas superfícies incisal do incisivo central direito e cúspides dos segundos molares para permitir as mensurações lineares das réplicas através do “sofware” Auto CAD. A avaliação dos deslocamentos dos dentes artificiais para ambos os grupos foi realizadas em diferentes momentos: a) dentadura encerada, b) dentadura encerada e parcialmente incluída com gesso ou silicone, c) após completa inclusão, d)dentadura incluída após a polimerização, e) dentadura aderida ao modelo após a demuflagem, f) dentadura separada do modelo, g) dentadura após o acabamento e polimento, h) após a permanência em água a 36º durante sete dias. Resultados: Ocorreram alterações lineares no posicionamento dos dentes artificiais para ambos os grupos e em todos os períodos da inclusão, com maior alteração para o grupo G2. Os dados das fases c e d foram significantemente diferentes das outras fases quando comparados intra grupos, para ambos os métodos de inclusão avaliados. Conclusão: O grupo G2 exibiu as maiores alterações. Após a polimerização, o grupo G1(inclusão com barreira de silicone complementada com gesso tipo III) demonstrou as maiores distâncias entre os pontos (expansão) enquanto o grupo G2 revelou as distâncias reduzidas (contração).

The dimensional alterations of denture base jeopardize adaptation and retention associated to altered occlusion, which are considered relevant factors forfabrication of complete dentures. Objective: The aimof this study was to evaluate linear tooth displacement during processing of maxillary complete dentures submitted to two inclusion methods and conventional polymerization. Material and Methods: Twenty maxillary complete dentures were fabricated: G1 –inclusion with silicone barrier, and G2 – inclusion with dental stone type IV barrier. Points were markedon incisal surface of right central incisor and cusp of second molars to allow linear measurement ofthe replicas through the software AutoCAD. The evaluation of artificial teeth displacement for both groups was carried out in different moments: a) waxed denture; b) waxed denture partially investedwith stone or silicone barrier; c) after complete flasking; d) denture into flask after polymerization;e) denture attached to the cast after deflasking; f)denture separated from the cast; g) denture after finishing and polishing; h) denture after storagein water at 36° during 7 days. Results: Linear alterations occurred on artificial teeth positioning for both groups and in all periods of inclusion, with higher alterations for group G2. The data from phasesc and d were significant different from other phases when compared intra groups, for both inclusion methods evaluated. Conclusion: Group G2 exhibited the greatest alterations. After polymerization, groupG1 (Inclusion with silicone barrier complementedwith dental stone type III) demonstrated higher distance between the points (expansion) while groupG2 revealed reduced distance (contraction).

Análise in vitro da proliferação celular e produção de mediadores inflamatórios por células da conjuntiva estimuladas por materiais utilizados na confecção de próteses oculares / In vitro analysis of cell proliferation and the production of inflammatory mediators by conjunctiva cells stimulated by ocular prosthesis materials

A prótese ocular é utilizada para a reabilitação estética e funcional da ausência ocular. O conhecimento da sua biocompatibilidade é importante para a utilização sem reações danosas aos usuários. O objetivo neste estudo foi avaliar a citotoxicidade de materiais utilizados na confecção de próteses oculares, por meio da análise da proliferação celular e da produção de citocinas pró-inflamatórias e de proteínas de matriz extracelular por células da conjuntiva humana. Inicialmente, foi analisada a influência de diferentes períodos de formação e de exposição dos extratos de resina acrílica branca (N1), termopolimerizada em água aquecida, sobre culturas de células da conjuntiva. Foram confeccionados 24 corpos de prova em resina, sendo 12 para cada período de exposição de células da conjuntiva aos extratos da resina testada (24 e 72 horas). Após a formação dos extratos por 24, 48 e 72 horas de imersão em meio de cultura e, 24 horas em água seguido de 24 horas de imersão em meio, os ensaios propostos foram realizados (n=3). Em seguida, foi avaliado o efeito citotóxico de diferentes métodos de polimerização de resina acrílica N1 em células da conjuntiva. Foram confeccionados 9 corpos de prova em resina (n=3), termopolimerizados em água aquecida, por energia de microondas ou ativados quimicamente, utilizados para a formação dos extratos dessas resinas. Os extratos foram obtidos após 72 horas de imersão dos corpos de prova em meio de cultura e, então, expostos às células da conjuntiva por 72 horas para a realização dos ensaios propostos. Adicionalmente, foi analisada a influência da presença do pigmento acrílico na confecção da prótese de resina acrílica N1, termopolimerizada em água aquecida. Foram confeccionados 9 corpos de prova (n=3): resina N1, resina N1 + pigmento e, pigmento, utilizados para a formação dos extratos desses materiais. Os extratos formaram-se por 72 horas de imersão dos corpos de prova em meio de cultura e, então, foram expostos às células da...

Ocular prosthesis is a treatment option for esthetical and functional rehabilitation of ocular absence. The knowledge of ocular prosthesis material’s biocompatibility is important to ensure a safe use in patients. The aim of this study was to evaluate the cytotoxic effect of ocular prosthesis materials, through the analysis of the cell proliferation, and the production of proinflammatory cytokines and extracellular matrix proteins by a human conjunctival cell line. Initially, the influence of different preparation and exposition periods of eluates from heat-polymerized ocular prosthesis N1 color acrylic resin in human conjunctival cell line was evaluated. A total of 24 acrylic resin samples were manufactured and divided into 2 groups, according to the eluate exposition period to conjunctival cell line (24 and 72 hours). Eluates corresponding to 24, 48 and 72 hours of resin sample immersion in medium and, 24 hours of resin sample immersion in water followed by 24 hours of immersion in medium, were prepared (n=3) for the proposed tests. Then, the cytotoxic effect of different polymerization methods of ocular prosthesis N1 color acrylic resin was analyzed. A total of 9 acrylic resin samples were manufactured (n=3), according to the polymerization method: heat-polymerization in water bath, polymerization by microwave energy and auto-polymerization. Eluates corresponding to 72 hours of resin sample immersion in medium were prepared for proposed tests and exposed to conjunctival cell line for 72 hours. Additionally, the influence of pigment incorporation on the cytotoxicity of heat-polymerized ocular prosthesis N1 color acrylic resin was evaluated. A total of 9 samples were manufactured (n=3): N1 color acrylic resin without pigment incorporation, N1 color acrylic resin with pigment incorporation, and acrylic pigment. Eluates corresponding to 72 hours of sample immersion in medium were prepared and exposed to conjunctival cell line for 72 hours. The cytotoxic effect...