Effect of Acute and Cumulative Stress on Gene Expression in Mammary Tissue and Their Interactions with Physiological Responses and Milk Yield in Saanen Goats.

This study addresses the hypothesis that different acute stressors can cumulatively decrease milk yield. In fact, in a time of global warming, the impact of environmental stress and farm management practices on milk production remains unclear. In this context, our objective was to investigate the effect of acute and cumulative stress on gene expression in mammary tissue and their interactions with physiological responses and milk yield in Saanen goats. Thirty lactating goats were subjected to two treatments: (1) control (CT), in which goats were maintained following a habitual routine under comfort conditions; (2) stress (ST), in which the goats were subjected to different types of environmental stress: heat stress, adrenocorticotropic hormone administration, hoof care management, and exposure to rain. These stressors were performed sequentially, with one stress per day on four consecutive lactation days, to evaluate their effect on milk quality and milk yield. Our results showed that compared to CT goats, cumulative stress increased the gene expression of glucocorticoid receptor (GR), interferon-gamma (IFN-γ), superoxide dismutase (SOD), and catalase (CAT) in mammary tissue, which are indicators of cortisol action, inflammatory response, and antioxidant enzymes. Furthermore, the acute challenges imposed on ST goats changed their rectal temperature and respiratory frequency and increased cortisol, glucose, cholesterol, triglycerides, and high-density lipoprotein release in plasma when compared to CT goats. Although these physiological and metabolic responses restore homeostasis, ST goats showed lower milk yield and higher somatic cell count in milk than CT goats. In conclusion, the results confirmed our initial hypothesis that different acute stressors cumulatively decrease the milk yield in Saanen goats.

Effect of experimental stress and cortisol release induced by ACTH administration on expression of key genes related to milk synthesis and apoptosis during mammary involution of Saanen goats.

This research paper addresses the hypothesis that stress, induced by ACTH administration and cortisol release increases somatic cell count (SCC) in mammary secretion, and improves the effectiveness of dry off in goats. We report indicators of milk synthesis and mammary gland involution during dry off. Thirty Saanen goats were subjected to abrupt dry off and treatments: (1) ACTH administration (ACTH) or (2) placebo (Control) on days 1, 3, 6, 9, 12, 15, 30, and 60 of dry off. The expression of target genes in mammary tissue that are related to milk synthesis and cell survival such as insulin-like growth factor 1 receptor (IGF1R), phosphatidylinositol-3-kinase (PIK3CA), protein kinase B (AKT1) and mechanistic target of rapamycin (MTOR), casein (CSN2), lactalbumin (LALBA) and lactoferrin (LF) were evaluated, and plasma cortisol concentration, SCC, leucocyte count, and microbiological analyses in milk and mammary secretions were assessed. ACTH significantly downregulated the expression of IGF1R and upregulated the expression of PIK3CA in mammary tissue, increased lactoferrin concentration and SCC, and changed immune cell levels in mammary secretions compared to Control. Furthermore, ACTH administration increased the percentage of dry goats compared to the Control (73 vs. 46%, respectively). We conclude that the effect of stress via ACTH administration and cortisol release accelerated mammary involution during the early dry-off period.

O Enfermeiro na saúde da família e a promoção de saúde e prevenção de doenças / The family health strategy nurse and health promotion and disease prevention

Objetivo: descrever as ações do enfermeiro para a promoção de saúde e prevenção de doenças. Metodologia: estudo exploratório, descritivo, transversal, de abordagem qualitativa, realizado com 12 enfermeiros das Estratégias de Saúde da Família de uma cidade do Triângulo Mineiro. Os dados foram obtidos por meio de entrevista individual semiestruturada e foram submetidos à Análise Temática. Resultados: as temáticas identificadas foram: visão dos enfermeiros sobre promoção de saúde e estratégias de intervenção; conhecimento dos enfermeiros quanto à prevenção de doenças e intervenções realizadas para esta finalidade. Conclusão: evidenciou-se a confusão de alguns enfermeiros em distinguir promoção de saúde de prevenção de doenças. Além disso, os dados demonstraram que os enfermeiros enfrentam desafios no cotidiano de trabalho que podem acarretar implicações para a prática. (AU)

Objective: To describe nurses´ actions for health promotion and disease prevention. Methodology: Exploratory, descriptive, and transversal study, using a qualitative approach, carried out with 12 nurses from the family health strategies of a city in the state of Minas Gerais. Data were obtained through semi-structured interviews and were submitted to thematic analysis. Results: The themes identified were: vision of nurses on health promotion and their intervention strategies; Knowledge of nurses regarding the prevention of diseases and the interventions carried out for this purpose. Conclusion: Results showed the confusion of some nurses in distinguishing health promotion and disease prevention. They also face challenges in the daily work that can entail implications for the practice. (AU)

Objetivo: describir las acciones de la enfermera para la promoción de la salud y la prevención de enfermedades. Metodologya: estudio exploratorio, descriptivo, transversal, enfoque cualitativo, realizado con 12 enfermeros de las estrategias de salud familiar de una ciudad del triángulo minero. Los datos se obtuvieron por medio de una entrevista individual semiestructurada y fueron analizados por medio de análisis temático. Resultados: los temas identificados fueron: visión de las enfermeras sobre promoción de la salud y sus estrategias de intervención; El conocimiento de las enfermeras en cuanto a la prevención de enfermedades y las intervenciones llevadas a cabo con este fin. Conclusión: se ha puesto de relieve la confusión de algunas enfermeras en la distinción de la promoción de la salud de la prevención de enfermedades. Enfrentan desafíos en el trabajo diario que resultar en implicaciones para la práctica. (AU)

Occurrence of acid erosion of the teeth, actinomycosis and polioencephalomalacia in flock of sheep fed wet low pectin citrus pulp / Ocorrência de erosão ácida dos dentes, actinomicose e polioencefalomalacia em rebanho de ovinos alimentados com polpa cítrica úmida despectinada

The citrus pulp can be used as a substitute in ruminant feed reducing costs and maintaining the nutritional quality of food. However, this compound should be used carefully so as not to cause harm to the animals. The present report aims to describe the occurrence of dental erosion, actinomycosis and polioencephalomalacia in sheep raised and kept with a wet low pectin citrus pulp based diet, composing 50% of roughage. Actinomycosis was diagnosed in five animals through clinical and radiographic examinations and microbiological culture, and, after treatment, three animals were cured. Polioencephalomalacia was confirmed in ten animals by clinical diagnostics, in nine out of ten animals by therapeutic diagnosis, and in one animal by post-mortem anatomopathological examination. According to the observed, we recommend caution when large amounts of citrus pulp are used as bulky food.(AU)

A polpa cítrica está entre os produtos que podem ser utilizados como substitutos na alimentação de ruminantes, diminuindo os gastos e mantendo a qualidade nutricional do alimento fornecido aos animais, porém, esses alimentos devem ser utilizados de forma que não tragam malefícios. Assim, o presente relato visa apresentar a ocorrência de erosão dentária, actinomicose e polioencefalomalácia em ovinos criados e mantidos recebendo alimentação à base de polpa cítrica úmida despectinada na concentração de 50% do volumoso. A actinomicose foi diagnosticada em cinco animais por meio de exame clínico, radiográfico e cultivo microbiológico, e após tratamento três animais foram curados. Já a polioencefalomalácia foi confirmada em dez animais pelos sintomas manifestados, eficiência da terapia instituída em nove animais e exame anatomopatológico de um animal que veio a óbito. De acordo com o observado, deve-se ter cuidado ao utilizar grande quantidade de polpa cítrica úmida como volumoso.(AU)