RESUMO
A novel nanocomposite based on TiO(2)-protein for functional applications was prepared and characterized. The composite was made by covalent immobilisation of bovine serum albumin (BSA) on to the TiO(2) particles. BSA was attached to the TiO(2) powder through a three-step process. This involves the functionalization of the TiO(2) particles with silane and the subsequent coupling by glutaraldehyde to free NH(2) groups of the protein. Formation of Schiff's base was confirmed by IR spectroscopy and the optimum loading of the BSA was found to be 73.61%. Surface morphology of the composite was studied by SEM and TEM. Thermal analysis of TiO(2)-BSA composite was carried out by TGA and DSC. Structural variation of the BSA after immobilisation was studied by CD spectra.
Assuntos
Nanocompostos/química , Soroalbumina Bovina/metabolismo , Titânio/química , Animais , Varredura Diferencial de Calorimetria , Bovinos , Dicroísmo Circular , Proteínas Imobilizadas , Nanocompostos/ultraestrutura , Bases de Schiff/química , Silanos/química , Termogravimetria , Difração de Raios XRESUMO
The synthesis of modified hydrophobic starch nanoparticles using long chain fatty acids was accomplished. Grafting of fatty acid on the starch was done using potassium persulphate as catalyst and the formation of graft polymer was confirmed by FTIR spectra. The thermal properties of the native and grafted starch were investigated using simultaneous TG-DTA and DSC. The graft polymerization was found to be depending on the temperature and the duration of the reaction. The modified starch nanoparticles were cross-linked with sodium tripoly phosphate for better stabilization. Morphology of the grafted starch nanoparticles was studied by SEM and AFM. Drug-loading and the controlled release of the drug from the nanoparticles was studied using indomethacin as model drug.