RESUMO
BACKGROUND: The possibility of inter-human transmission of Mycobacterium abscessus in cystic fibrosis centres has been recently hypothesized suggesting the need for the molecular characterization of strains isolated from such patients. MATERIALS AND METHODS: One hundred and forty one isolates of M. abscessus grown from sputum samples of 29 patients with cystic fibrosis were genotyped resorting to variable number of tandem repeats (VNTR) determination and whole genome sequencing (WGS). RESULTS: Out of 29 VNTR profiles, 15 were unique to the same number of patients while seven were shared by multiple patients. WGS showed that only two of the patients sharing common VNTR patterns were indeed infected by the same strain. The shared VNTR patterns were mostly present among the isolates of M. abscessus subsp. abscessus. CONCLUSION: As expected WGS showed a clearly higher discriminatory power in comparison with VNTR and appeared the only molecular epidemiology tool suitable to effectively discriminate the isolates of M. abscessus subsp. abscessus.
Assuntos
Fibrose Cística/microbiologia , Mycobacterium abscessus/genética , Mycobacterium abscessus/isolamento & purificação , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Técnicas de Tipagem Bacteriana , RNA Polimerases Dirigidas por DNA/genética , RNA Polimerases Dirigidas por DNA/metabolismo , Genótipo , Humanos , Repetições Minissatélites , Mycobacterium abscessus/classificação , Mycobacterium abscessus/enzimologia , Filogenia , Escarro/microbiologiaAssuntos
Complexo Mycobacterium avium/genética , Infecção por Mycobacterium avium-intracellulare/diagnóstico , Tuberculose Pulmonar/diagnóstico , Idoso , Feminino , Genes Bacterianos , Humanos , Itália , Dados de Sequência Molecular , Complexo Mycobacterium avium/isolamento & purificação , Análise de Sequência de DNARESUMO
Cobas Amplicor MTB and later Cobas TaqMan MTB were used to test a very large series of consecutive specimens received for tuberculosis diagnosis. Performance parameters were estimated and compared overall and for separate specimen categories. Both systems showed excellent specificity, and that of TaqMan was the higher. The sensitivities were similar but satisfactory only with respiratory specimens and smear-positive samples.
