Densitometric method for assessment of six specialized metabolites in four Sida sp. and its congener Abutilon indicum: Targeted metabolomics, greenness assessment, and chemometrics analysis.

Sida is one of the most diverse genera, with about 200 species distributed in tropical and subtropical regions of the world. Among 18 species distributed in India, Sida acuta, Sida cordifolia, Sida rhombifolia, and Sida cordata are used in traditional medicines along with its possible adulterant Abutilon indicum for several therapeutic uses. The non-availability of marker-based validated methods for the identification and classification of these species leads to adulteration. Indoloquinoline and quinazoline are the major bioactive alkaloids distributed in Sida spp. First time, a simple, economical and high throughput method was developed and validated for the simultaneous determination of 20-hydroxyecdysone (1), vasicine (2), vasicinone (3), cryptolepine (4), quindolinone (5), and cryptolepinone (6) using HPTLC-UV densitometry. The method was validated to meet globally accepted ICH guidelines. The method was sensitive with LOD and LOQ ranging from 0.38-0.63 and 1.57-2.12 µg/band. The samples were spiked at 3 different concentrations, the recovery values were 93.49-98.88%. In addition, the greenness index of the HPTLC method was estimated using four different greenness assessment techniques. Targeted HPTLC analysis indicated the distribution of specialized metabolites in Sida spp. and A. indicum. However, the occurrence of cryptolepine in A. indicum was not reported in the literature, so this was further confirmed by liquid chromatographic studies of the samples from different locations. The chromatographic data was statistically evaluated by principal component analysis (PCA) and hierarchical clustering (HCA). HPTLC-based targeted metabolite quantitation explains the adulteration/substitution in Sida raw material and derived herbal preparations.

Cepstral Characteristics of Voice in Indian Female Classical Carnatic Singers.

BACKGROUND: The cepstral measures are gaining attention in the voice analysis as it is reported to be strong predictor of voice impairments. As singer's voice is reported to be present with more harmonics, it can be hypothesized that there would be an increase in the value of cepstral parameters. Also, little is known about the cepstral characteristics of voices in Indian classical Carnatic singers; and hence, the present study was undertaken with an aim to characterize the singer's voice using cepstral measures. METHODS: Thirty Indian female classical Carnatic singers and 30 nonsingers participated in the study. Phonation of vowel /a/ was recorded at their habitual pitch and loudness. The cepstral peak prominence and smoothened cepstral peak prominence were analyzed using the Hillenbrand algorithm available in Speech Tool software. Mean and standard deviation was calculated for each group. Obtained scores were analyzed using independent t test. RESULTS: Mean raw scores were found to be increased in classical Carnatic singers in comparison with nonsingers. Results of independent t test revealed significant difference between the means of singers and nonsingers for both the cepstral measures at P < 0.05. CONCLUSIONS: The present study investigated the cepstral characteristic of voice in singers and nonsingers. Results revealed that there was a significant difference between the means of singers and nonsingers indicating that cepstral parameters were higher among the singers in comparison with nonsingers. The obtained results were attributed to the harmonic organization in the voices of singers.