Zizyphus mauritiana seed extract: Paving the way for next-generation bone constructs with nano-fluorohydroxyapatite/carboxymethyl chitosan nanocomposite scaffold.

This is the first study that explored the potential use of Zizyphus mauritiana seed extract (ZSE) to synthesize nano-fluorohydroxyapatite/carboxymethyl chitosan nanocomposite scaffolds at different concentrations (CFZ1, CFZ2 and CFZ3) using co-precipitation method. The proposed scaffolds showed presence of intermolecular H bonding interactions between the constituents, according to the FTIR. The mechanical studies revealed shore hardness of 72 ± 4.6 and optimal compressive modulus in case of CFZ3 [1654.48 ± 1.6 MPa], that was comparable with that of human cortical bone. The SEM, TEM and platelet adhesion images corroborated uniformly distributed needle like particles in case of CFZ3 with an average size ranging from 22 to 26 nm, linked rough morphology, and appropriate hemocompatibility. The markedly up regulation in the ALP activity and protein adsorption upon increasing ZSE concentration demonstrated that CFZ nanocomposite scaffolds were compatible with osteoblastic cells relative to CF nanocomposite. The cytotoxicity study indicated that CFZ nanocomposite do not induce toxicity over MG-63 and did not aggravate LDH leakage in contrast to CF. The histopathological investigations on albino rats confirmed significantly improved regeneration of bone in the repair of a critical-size [8 mm] calvarium defect. Therefore, CFZ3 nanocomposite scaffold represents a simple, off-the-shelf solution to the combined challenges associated with bone defects.

Synthesis and application of manganese-doped zinc oxide as a potential adsorbent for removal of Congo red dye from wastewater.

Synthetic dyes are considered toxic compounds and as such are not easily removed by conventional water treatment processes. This study demonstrated the synthesis of pure and manganese- (Mn), silver- (Ag), and iron- (Fe) doped zinc oxide (ZnO) nanoparticles via the wet chemical route. In particular, it investigated the batch adsorption studies and physiochemical properties of synthesized pure and doped ZnO materials for removing toxic congo red (CR) dye. X-ray diffraction (XRD), Raman spectroscopy, and energy-dispersive X-ray spectroscopy (EDS) confirmed the synthesis of the pure and doped ZnO materials. The batch adsorption investigation revealed adsorption efficiencies of 99.4% for CR dye at an optimal dose of 0.03 g/30 ml for Mn-doped ZnO at a solution pH of 2. The adsorption capacity of each of the synthesized materials was found to be in order Mn-doped ZnO (232.5 mg/g) > Ag-doped ZnO (222.2 mg/g) > pure ZnO (212.7 mg/g) > Fe-doped ZnO (208.3 mg/g). Both pseudo-second-order kinetics model and the Langmuir isotherm model accurately explained the adsorption behaviors of CR dye. As such, Van der Waal interactions, H-bonding, and electrostatic interaction were found to be the adsorption mechanisms responsible for dye removal. In addition, the desorption-regeneration investigation indicated the successful reuse of the exhausted Mn-doped ZnO material for five cycles of CR dye adsorption with an efficiency of 83.1%. Overall, this study has demonstrated that Mn-doped ZnO could be considered a viable adsorbent for the cleanup of dye-contaminated water.

Development of a ZnOS+C Composite as a Potential Adsorbent for the Effective Removal of Fast Green Dye from Real Wastewater.

Wastewater treatment is becoming increasingly important due to the potential shortage of pure drinking water in many parts of the world. Adsorption offers a potential technique for the uptake of contaminants and wastewater purification. In the last two decades, several efforts have been made to remove fast green (FG) dye from wastewater via different adsorbent materials. However, adsorption capacity shown by these adsorbents is low and time-consuming. Herein, we have synthesized for the first time a new powdered adsorbent ZnOS+C, modified zinc peroxide with sulfur and activated carbon to effectively remove FG dye from wastewater. Results of batch adsorption experiments have suggested that ZnOS+C has the maximum adsorption potential of 238.28 mg/g for FG dye within 120 min of adsorption equilibrium for a wide range of pH ranging from 2 to 10 pH. The adsorption process conforms to the Freundlich isotherm model, suggesting a multilayered adsorption process on the outer surface of ZnOS+C. The adsorption kinetics study indicates that the kinetics of the reaction are the intraparticle diffusion model. Briefly, this study shows proof of the application of ZnOS+C powder as a new eco-friendly adsorbent with extremely high efficiency and high surface area for removing FG dye.

Green synthesis of zinc oxide nanoparticles using lychee peel and its application in anti-bacterial properties and CR dye removal from wastewater.

In nanoscience and nanobiotechnology, using plant extracts in synthesizing metal nanoparticles (NPs) has recently come to light as an exciting opportunity with several benefits over traditional physicochemical methods. In the present work, zinc oxide (ZnO) based nanoparticles (NPs) were synthesized by green chemistry route using lychee peel extract to capture hazardous congo red dye from wastewater and illustrate their antimicrobial behavior. The X-Ray Diffraction (XRD) spectra confirm the wurtzite crystal structure, and Fourier Transform Infrared (FTIR) spectra confirm the functional group in ZnO, which is suitable for dye adsorption. It was found that the NPs were spherical and had a size of <10 nm. The synthesized ZnO NPs could effectively remove >98% of CR dye from wastewater within 120 min of contact time at a wide pH range from 2 to 10. The primary mechanism involved in removing dye was the electrostatic interaction between ZnO adsorbent and CR dye. The antimicrobial performance of synthesized ZnO NPs was found to show 34% inhibition against Bacillus subtilis (ATCC 6538), 52% against Escherichia coli (ATCC 11103), 58% against Pseudomonas aeruginosa (ATCC 25668) and 32% against Staphylococcus aureus (ATCC 25923) using well diffusion assay. ZnO demonstrates a suitable anti-bacterial property over both gram-positive and gram-negative pathogenic bacteria. Overall, the green synthesized method for developing ZnO NPs shows promising and significant anti-bacterial performance and is a highly potential adsorbent for removing CR dye from wastewater.

Fast and Effective Removal of Congo Red by Doped ZnO Nanoparticles.

ZnO nanoparticles (NPs) show remarkable efficiency in removing various contaminants from aqueous systems. Doping ZnO NPs with a second metal element can dramatically change the physicochemical properties of the pristine nanoparticles. However, there have been limited reports on the absorption of doped ZnO NPs, especially comparing the performance of ZnO NPs with different doping elements. Herein, ZnO NPs were doped with three transitional metals (Co, Fe, and Mn) at a nominal 2 wt.%. The particle surface had a higher dopant concentration than the interior for all NPs, implying the migration of the dopants to the surface. Because doping atoms inhibited grain growth, the doped ZnO NPs had a small particle size and a large surface area. The adsorption performance followed the order of Fe-doped < undoped < Mn-doped < Co-doped ZnO. Co-doped ZnO had an increased surface area and less tendency to agglomerate in an aqueous solution, showing the best adsorption performance. The adsorption of Congo red (CR) on Co-doped ZnO followed the pseudo-second-order model and the Langmuir isotherm. The adsorption process was spontaneous through monolayer chemisorption, and the maximum adsorption capacity was 230 mg/g. Finally, the Co-doped ZnO was successfully incorporated into an alginate membrane by electrospinning. The membrane demonstrated excellent adsorption performance and had great potential as an innovative and low-cost adsorbent (inexpensive raw materials and simple processing) for wastewater purification.

Anthropogenic aerosols in precipitation over the Indo-Gangetic basin.

This study investigated the concentration of heavy metals in rainwater (RW) at a semi-arid region of the Indo-Gangetic basin to understand the influence of local, regional, or long-range transport of air pollutants during the monsoon and non-monsoonal rain. The concentration of heavy metals in RW was determined using Atomic Absorption Spectrophotometer with Graphite Furnace, the scavenging ratio was estimated, and source interpretation was carried out using Principle Component Analysis (PCA) and HYSPLIT model. Ca was the highest contributor in RW followed by Na, Fe, Mg, and Al whereas Ba, Cr, Cu, Mn, Ni, Pb, and Zn were found in trace quantity. During the non-monsoon period, the crustal component (Ca) was the highest; however, during the monsoon, sea salt components (Na and Fe) were found higher. The scavenging ratio for metals was estimated and was found many times higher than those reported over European sites. The moderate concentration of heavy metal in RW was found with higher wind from South (S), South-West (SW), and North-West (NW) directions. Air mass back trajectory shows a significant contribution of metals from the Arabian Sea (South-Westerly wind) during active monsoon, whereas, in the non-monsoon season, the air masses mainly originated from the north-west indicating a contribution from wind-blown dust. The correlation analysis has shown the positive correlations between Ca and Mg, Mg and Na, Na and Cu, Al and Zn, Zn and Ba, Ba and Cr, and Cr and Zn. Principal Component Analysis (PCA) indicated loading of Ca, Na, Mg, Cu, Mn, and Ni in the first factor suggesting their crustal origin, whereas the second factor showed high loading of Al, Ba, Zn, Cr, and Ni indicating vehicular exhaust and industrial emission as their major sources, and loading for Ba and Mg in the third factor indicates the mixed contribution from both natural and anthropogenic sources in rainwater during the monsoon and non-monsoon periods. The data of this study can be used in the air pollution transport model. This study will help in source interpretation over the Indo-Gangetic basin and will help in planning for National Clean Air Program (NCAP) and deriving critical load.

Sodium Docusate Surface-Modified Dispersible and Powder Zinc Peroxide Formulation: An Adsorbent for the Effective and Fast Removal of Crystal Violet Dye, an Emerging Wastewater Contaminant.

Crystal violet (CV) dye is one of the most toxic dyes majorly generated by textile industries. It may cause health issues if enters human beings. A lot of research has been reported for the removal of CV dye from wastewater; however, most of them are time-consuming and hardly remove more than 95% of the CV dye. In the last few years, we have tested several materials, and most of them have exhibited very low efficacy toward adsorption of CV including zinc peroxide (ZnO2). To enhance adsorption efficacy, dispersibility, and stability, the surfaces of several reported materials were modified using different wetting agents and nonionic surfactants. Interestingly, ZnO2, which was earlier very less effective after surface modification by sodium salt of dioctyl sulfosuccinate, efficiently adsorbed >99.5% of CV from contaminated water within 5 min of contact time at pH ∼10. The adsorption capacity obtained for the sodium docusate surface-modified zinc peroxide (ZnSD) adsorbent was found to be 123 mg/g, which is much better than the other reported for CV removal. Different physiochemical experiment parameters like pH, contact time, initial dye concentration, adsorbent dosages, and temperature were optimum to achieve maximum adsorption of the CV dye. The adsorption rate and adsorption mechanism studies show that the adsorption of CV follows pseudo-second-order kinematics and the Freundlich isotherm model. The adsorption results are consistent, and even treated water can be reutilized for various applications.

Development of a pH-sensitive functionalized metal organic framework: in vitro study for simultaneous delivery of doxorubicin and cyclophosphamide in breast cancer.

Exploration of an efficient dual-drug based nanocarrier with high drug loading capacity, specific targeting properties, and long-term stability is highly desirable in cancer therapy. Metal-organic frameworks (MOFs) have proven to be a promising class of drug carriers due to their high porosity, crystalline properties with defined structure information, and their potential for further functionalization. To enhance the drug efficacy as well as to overcome the burst effect of drugs, here we synthesized a pH responsive folic acid (FA) and graphene oxide (GO) decorated zeolitical imidazolate frameworks-8 (GO-FA/ZIF-8), for targeted delivery of doxorubicin (DOX) and cyclophosphamide (CP), simultaneously. In this system, DOX molecules were encapsulated in the pores of ZIF-8 during in situ synthesis of ZIF-8 and CP molecules have been captured by the GO surface via hydrogen bonding and π-π interactions as well. Furthermore, the resulting pH-responsive nanocarrier (DOX@ZIF-8/GO-FA/CP) showed in vitro sustained release characteristics (76% of DOX and 80% of CP) by cleavage of chemical bonding and disruption of the MOFs structure under acidic condition (at pH 5.6). Moreover, DOX@ZIF-8/GO-FA/CP has synergistic cytotoxic effects as compared to the combination of both the drugs without ZIF-8/GO-FA when treating MCF-7 and MDA-MB-231 breast cancer cell lines (with a combination index of 0.29 and 0.75 for MCF-7 and MDA-MB-231 cell-lines, respectively). Hence this system can be applied as an effective platform for smart dual drug delivery in breast cancer treatment through its remarkable manageable multidrug release.

The removal of pentavalent arsenic by graphite intercalation compound functionalized carbon foam from contaminated water.

In the present investigation, Graphite intercalation compound (GIC) functionalized phenolic resin based carbon foam for removal of arsenic (As(V)) from contaminated water is developed by sacrificial template technique followed by carbonization at 1000 °C in N2. The GICCF adsorbent is characterised by scanning electron microscope (SEM) for morphological study, X-ray diffraction (XRD) patterns explains the phase information and interlayer spacing of the adsorbent, whereas Fourier Transform Infrared Spectroscopy (FTIR) and X-ray Photoelectron Spectroscopy (XPS) gives the information about surface functionality and mechanism of adsorption of As(V) over the surface of adsorbent. The time data is fitted well in pseudo second order kinetics and follows multilinear nature of intra-particle diffusion model. The adsorption nature of adsorbent and adsorbate is explained by Langmuir isotherm better than Freundlich isotherm, Temkin isotherm, and D-R isotherm. The adsorption capacity of adsorbent is 62.5 µgg-1, which is calculated by Langmuir isotherm. Arsenic removal by GICCF is taken place within two hrs up to acceptable limit. The proposed GICCF can be regenerated after treating with 0.1 M HNO3 and 0.1 M HCl solution and it can be used for multiple times.

Iron acquisition in maize (Zea mays L.) using Pseudomonas siderophore.

Siderophore facilitates iron availability in soil, but its assistance in iron transportation to different plant parts is not reported till date. Therefore, it is worthwhile to study the effect of siderophore produced by Pseudomonas for iron acquisition in the presence and absence of iron. To study these effects, two siderophore-producing Pseudomonas aeruginosa strains RSP5 and RSP8 were selected. RSP5 and RSP8 produced the highest and lowest amounts of siderophore, respectively. Iron (Fe) concentration of stem, leaf, seed, and shoot length, root length, cob length, and number of grains parameters were analysed. It was observed that the plants treated with RSP5 were sturdier and taller than RSP5 + Fe > RSP8 > RSP8 + Fe > Fe > Control plants. Iron content of RSP8 vs. RSP8 + Fe, RSP8 + Fe vs. Control, and RSP8 + Fe vs. RSP5 + Fe was significantly different (P < 0.01). Analysis of variance (ANOVA) proves that RSP5 was able to transport higher amount of iron to maize plant than other treatments. Increase in shoot length, root length, cob length, grain number and iron content of stem, and leaf and seed of maize plant inoculated with RSP5 suggests that the strain can be used as an inoculant for increasing iron transportation in maize plant. (Indian Patent Filed: 40163/DEL/2016).

Chemical characteristics of trace metals in PM10 and their concentrated weighted trajectory analysis at Central Delhi, India.

Trace metals associated with PM10 aerosols and their variation during day and nighttime as well as during different seasons have been studied for the year 2012. PCA analysis suggested 5 PCs, which accounted for 86.8% cumulative variance. PC1 accounted for 30% with a significant loading of metals of anthropogenic origin, while PC2 showed 28% variance with the loading of metals of crustal origin. These trace metals showed seasonal distinct day and night time characteristics. The concentrations of Cu, Pb, and Cd were found to be higher during nighttime in all the seasons. Only Fe was observed with significantly higher mean concentrations during daytime of all seasons except monsoon. The highest mean values of Cu, Cd, Zn, and Pb during post-monsoon might be attributed to winds advection over the regions of waste/biomass burning and industrial activities in Punjab and Haryana regions. Furthermore, concentration weighted trajectory analysis suggested that metals of crustal origin were contributed by long-range transport while metals of anthropogenic and industrial activities were contributed by regional/local source regions.

Study of cyanide removal from contaminated water using zinc peroxide nanomaterial.

The present study highlights the potential application of zinc peroxide (ZnO2) nanomaterial as an efficient material for the decontamination of cyanide from contaminated water. A process patent for ZnO2 synthesis has been granted in United States of America (US Patent number 8,715,612; May 2014), South Africa, Bangladesh, and India. The ZnO2 nanomaterial was capped with polyvinylpyrrolidone (PVP) to control the particle size. The PVP capped ZnO2 nanomaterial (PVP-ZnO2) before and after adsorption of cyanide was characterized by scanning electron microscope, transmission electron microscope, X-ray diffractometer, Fourier transform infrared spectroscopy and time of flight-secondary ion mass spectrometry. The remaining concentration of cyanide after adsorption by PVP-ZnO2 was determined using ion chromatograph. The adsorption of cyanide over PVP-ZnO2 was also studied as a function of pH, adsorbent dose, time and concentration of cyanide. The maximum removal of cyanide was observed in pH range 5.8-7.8 within 15min. The adsorption data was fitted to Langmuir and Fruendlich isotherm and it has been observed that data follows both the isotherms and also follows second order kinetics.

Zinc peroxide nanomaterial as an adsorbent for removal of Congo red dye from waste water.

In the past decade, various natural byproducts, advanced metal oxide composites and photocatalysts have been reported for removal of dyes from water. Although these materials are useful for select applications, they have some limitations such as use at fixed temperature, ultra violet (UV) light and the need for sophisticated experimental set up. These materials can remove dyes up to a certain extent but require long time. To overcome these limitations, a promising adsorbent zinc peroxide (ZnO2) nanomaterial has been developed for the removal of Congo red (CR) dye from contaminated water. ZnO2 is highly efficient even in the absence of sunlight to remove CR from contaminated water upto the permissible limits set by the World Health Organization (WHO) and the United States- Environmental Protection Agency (US-EPA). The adsorbent has a specific property to adjust the pH of the test solution within 6.5-7.5 range irrespective of acidic or basic nature of water. The adsorption capacity of the material for CR dye was 208mgg-1 within 10min at 2-10pH range. The proposed material could be useful for the industries involved in water purification. The removal of CR has been confirmed by spectroscopic and microscopic techniques. The adsorption data followed a second order kinetics and Freundlich isotherm.

Adsorptional photocatalytic mineralization of oxytetracycline and ampicillin antibiotics using Bi2O3/BiOCl supported on graphene sand composite and chitosan.

In present study, heterojunctioned Bi2O3/BiOCl (BO/BOC) was synthesized via in situ chemical reduction and oxidation of BiOCl nanoplates. BiOCl was reduced to metallic Bi in KHB4 solution followed by oxidation in H2O2 solution to produce BO/BOC. The BO/BOC was supported over graphene sand composite and also on chitosan using wet impregnation method to report BO/BOC/GSC and BO/BOC/CT nanocomposite. The morphology and compositional characteristics of BO/BOC/GSC and BO/BOC/CT were investigated by FESEM, TEM, HRTEM, FTIR, XRD, EDX, RAMAN, BET and UV-visible diffuse reflectance spectral analysis. The photocatalytic activity of BO/BOC/GSC and BO/BOC/CT was performed for mineralization of ampicillin (AMP) and oxytetracycline (OTC) antibiotics under solar light. The adsorption process had significant effect on photodegradation of AMP and OTC. The adsorption of both OTC and AMP onto BO/BOC/GSC and BO/BOC/CT followed pseudo second order kinetics. Simultaneous adsorption and degradation process (A+P) resulted in higher degradation rate of investigated antibiotics. The applicability of power law model indicates the intricacies of mineralization process. During A+P process, OTC and AMP were mineralized to CO2·H2O, NO3(-) and SO4(2-) ions. Both BO/BOC/GSC and BO/BOC/CT exhibited significant recycle efficiency.

An in vitro comparison of effect on fracture strength, pH and calcium ion diffusion from various biomimetic materials when used for repair of simulated root resorption defects.

AIM: The aim of this study was to compare the effect on fracture strength, pH and calcium ion diffusion from mineral trioxide aggregate (MTA) Fillapex, iRoot SP, and Ultracal when used for repair of simulated root resorption defects. MATERIALS AND METHODS: Three sets of 40 teeth each were used, and biomechanical preparation was done. Resorption cavity was made at 5 mm from the apex. Teeth were filled with different experimental materials. In control group, saline was used. Samples of fracture resistance were stored in an incubator for 3 months and then subjected to the universal testing machine. To evaluate the pH and calcium ion release were checked at 1 day and 1, 2, 3, and 4 weeks intervals. Data were recorded and statistical analysis done by one-way analysis of variance followed by post-hoc Tukey test. RESULTS: Highest fracture resistance was seen in MTA Fillapex followed by iRoot SP, control, and then Ultracal group. Teeth filled with iRoot SP showed highest pH and calcium ion release followed by MTA Fillapex and Ultracal group. CONCLUSION: Bioceramic sealers showed high pH, calcium ion release, and good root reinforcement potential. Initial dressing of calcium hydroxide followed by obturation with Gutta-percha and bioceramic sealer may be considered as an alternative treatment modality for inflammatory resorption.

Quantifying uncertainty in measurement of mercury in suspended particulate matter by cold vapor technique using atomic absorption spectrometry with hydride generator.

As a result of rapid industrialization several chemical forms of organic and inorganic mercury are constantly introduced to the environment and affect humans and animals directly. All forms of mercury have toxic effects; therefore accurate measurement of mercury is of prime importance especially in suspended particulate matter (SPM) collected through high volume sampler (HVS). In the quantification of mercury in SPM samples several steps are involved from sampling to final result. The quality, reliability and confidence level of the analyzed data depends upon the measurement uncertainty of the whole process. Evaluation of measurement uncertainty of results is one of the requirements of the standard ISO/IEC 17025:2005 (European Standard EN IS/ISO/IEC 17025:2005, issue1:1-28, 2006). In the presented study the uncertainty estimation in mercury determination in suspended particulate matter (SPM) has been carried out using cold vapor Atomic Absorption Spectrometer-Hydride Generator (AAS-HG) technique followed by wet chemical digestion process. For the calculation of uncertainty, we have considered many general potential sources of uncertainty. After the analysis of data of seven diverse sites of Delhi, it has been concluded that the mercury concentration varies from 1.59 ± 0.37 to 14.5 ± 2.9 ng/m(3) with 95% confidence level (k = 2).

Evaluation of purity with its uncertainty value in high purity lead stick by conventional and electro-gravimetric methods.

BACKGROUND: A conventional gravimetry and electro-gravimetry study has been carried out for the precise and accurate purity determination of lead (Pb) in high purity lead stick and for preparation of reference standard. Reference materials are standards containing a known amount of an analyte and provide a reference value to determine unknown concentrations or to calibrate analytical instruments. A stock solution of approximate 2 kg has been prepared after dissolving approximate 2 g of Pb stick in 5% ultra pure nitric acid. From the stock solution five replicates of approximate 50 g have been taken for determination of purity by each method. The Pb has been determined as PbSO4 by conventional gravimetry, as PbO2 by electro gravimetry. The percentage purity of the metallic Pb was calculated accordingly from PbSO4 and PbO2. RESULTS: On the basis of experimental observations it has been concluded that by conventional gravimetry and electro-gravimetry the purity of Pb was found to be 99.98 ± 0.24 and 99.97 ± 0.27 g/100 g and on the basis of Pb purity the concentration of reference standard solutions were found to be 1000.88 ± 2.44 and 1000.81 ± 2.68 mg kg-1 respectively with 95% confidence level (k = 2). The uncertainty evaluation has also been carried out in Pb determination following EURACHEM/GUM guidelines. The final analytical results quantifying uncertainty fulfills this requirement and gives a measure of the confidence level of the concerned laboratory. CONCLUSIONS: Gravimetry is the most reliable technique in comparison to titremetry and instrumental method and the results of gravimetry are directly traceable to SI unit. Gravimetric analysis, if methods are followed carefully, provides for exceedingly precise analysis. In classical gravimetry the major uncertainties are due to repeatability but in electro-gravimetry several other factors also affect the final results.

Enzyme-modified indium tin oxide microelectrode array-based electrochemical uric acid biosensor.

We fabricated a miniaturized electrochemical uric acid biosensor with a 3-aminopropyltriethoxysilane (APTES)-modified indium tin oxide (ITO) microelectrode array (µEA). The ITO-µEA on a glass plate was immobilized with the enzyme uricase, through a cross-linker, bis[sulfosuccinimidyl]suberate (BS3). The enzyme-immobilized electrode (uricase/BS3/APTES/ITO-µEA/glass) was characterized by atomic force microscopy and electrochemical techniques. The cyclic voltammetry and impedance studies show an effective binding of uricase at the µEA surface. The amperometric response of the modified electrode was measured towards uric acid concentration in aqueous solution (pH 7.4), under microfluidic channel made of polydimethylsiloxane. The µEA biosensor shows a linear response over a concentration range of 0.058 to 0.71 mM with a sensitivity of 46.26 µA mM-1 cm-2. A response time of 40 s reaching a 95% steady-state current value was obtained. The biosensor retains about 85% of enzyme activity for about 6 weeks. The biosensor using µEA instead of a large single band of electrode allows the entire core of the channel to be probed though keeping an improved sensitivity with a small volume of sample and reagents.

Comparative study of leaching of silver nanoparticles from fabric and effective effluent treatment.

Nano silver (Ag(n)) is employed as an active antimicrobial agent, but the environmental impact of Ag(n) released from commercial products is unknown. The quantity of nanomaterial released from consumer products during use should be determined to assess the environmental risks of advancement of nanotechnology. This work investigated the amount of silver released from three different types of fabric into water during washing. Three different types of fabric were loaded with chemically synthesized Ag nanoparticles and washed repeatedly under simulated washing conditions. Variable leaching rates among fabric types suggest that the manufacturing process may control the release of silver reaching the waste water treatment plants. In an attempt to recover the Ag(n) for reutilization and to save it from polluting water, the effluents from the wash were efficiently treated with bacterial strains. This treatment was based on biosorption and was very efficient for the elimination of silver nanoparticles in the wash water. The process ensured the recovery of the Ag(n) leached into the effluent for reutilization, thus preventing environmental repercussions.

High yield synthesis of intrinsic, doped and composites of nano-zinc oxide using novel combinatorial method.

A novel synthesis of the production of luminescent zinc oxide (ZnO), either in its intrinsic, metal, non-metal-doped or composite forms with high yield has been developed by parallel iterative techniques, within a combinatorial library prepared by the reduction of nitroarenes. The reduction of nitroarenes by aluminium/zinc dusts in alkaline medium (pH 10±2) forms azoxy compounds, whereas in acidic medium (pH 4.9±0.2) forms phenyl hydroxylamine and zinc/aluminium dust gets oxidised into respective hydroxide. Here, we demonstrate the reduction of nitroarenes at neutral pH (7.0±0.2), which forms intrinsic as well as doped ZnO at 50±5°C using zinc dust alone or mixtures of salts of several transition and non-transition metals in presence of 1:10 ratio of solvent and water. Interestingly, it is observed that the photoluminescence emission could be tuned in a wide range from 390 to 615 nm useful for many display related devices.