Characterization, Antioxidant, and Antimicrobial Properties of Mulberry Lattices.

In order to find the most advantageous bioactive compounds from mulberry latex for drug development in the near future, this study was conducted to characterize and evaluate antioxidant and antimicrobial properties from four different mulberry lattices (BR-2, S-1, AR-14, and S-146). The characterization of the lattices was performed by scanning electron microscopy with energy-dispersive X-ray spectroscopy, gas chromatography coupled to mass spectroscopy, and Fourier transform infrared spectroscopy. Further, screenings of the antioxidant and antimicrobial potential of selected lattices were performed in vitro using 2,2-diphenyl-1-picrylhydrazyl assay and agar well diffusion methods, respectively. Interestingly, the outcome of the current study revealed that tested mulberry lattices contain a considerable amount of bioactive phytoconstituents, particularly antimicrobial and antioxidant compounds, as revealed by chromatographic analysis. BR-2 latex was found to have significant antioxidant activity (75%) followed by S-146 (64.6%) and AR-14 (52.9%). The maximum antimicrobial activity was found in BR-2 latex compared to other tested latex varieties. The results of this investigation showed that mulberry latex from the BR-2 type may successfully control both bacterial and fungal infections, with the added benefit of having enhanced antioxidant capabilities.

Weightbearing CT Analysis of the Transverse Tarsal Joint During Eversion and Inversion.

BACKGROUND: Understanding of the movement and function of the transverse tarsal joint (TTJt) continues to evolve. Most studies have been done in cadavers or under nonphysiologic conditions. Weightbearing computed tomographic (WBCT) scans may provide more accurate information about the position of the TTJt when the hindfoot is in valgus or varus. METHODS: Five volunteers underwent bilateral weightbearing CT scans while standing on a platform that positioned both hindfeet in 20 degrees of valgus and 20 degrees of varus. Each bone of the foot was segmented, and the joint surfaces of the talus, calcaneus, cuboid, and navicular were identified. The principal axes for each joint surface were determined and used to calculate the angles and distances between the bones with the foot in valgus or varus. RESULTS: In the coronal plane, the angle between the talus and calcaneus rotated 17.1 degrees as the hindfoot moved from valgus to varus. The distance between the centers of the talus and calcaneus decreased 7.1 mm. The cuboid translated 3.9 mm medially relative to the calcaneus. There was no change in angle or distance between the cuboid and navicular. The navicular rotated 25.4 degrees into varus relative to the talus. CONCLUSION: The TTJt locking mechanism was previously thought to occur from the talonavicular and calcaneocuboid joint axes moving from parallel to divergent as the hindfoot inverts. The current data show a more complex interaction between the four bones that comprise the TTJt and suggests that the locking mechanism may occur because of tightening of the ligaments and joint capsules. CLINICAL RELEVANCE: This study uses weight bearing CT scans of healthy, asymptomatic volunteers standing on valgus and varus platforms to characterize the normal motion of the transverse tarsal joint of the foot. A better understanding of how the transverse tarsal joint functions may assist clinicians in both the conservative and surgical management of hindfoot pathology.

Profile changes after conventional and chin shield genioplasty.

INTRODUCTION: The aim of this study was to compare the profile changes after conventional and chin shield genioplasty. MATERIAL AND METHOD: 20 patients with retruded chin were randomly allocated to two different groups. The experimental group had chin shield osteotomy with interposition of hydroxyapatite collagen graft soaked in platelet rich plasma, while the controls had a conventional genioplasty. The outcome variables evaluated were lip seal, chin thickness, mandibular base length, SNB, labiomental angle, anterior lower facial height, transverse chin shift, and complications. RESULTS: There was an increase in chin thickness among all, but a significant increase in anterior lower facial height was seen in the experimental group only. There was no statistically significant difference in satisfaction score in both groups. CONCLUSION: Chin shield genioplasty provides horizontal as well as vertical lengthening of chin without deepening of the mentolabial fold. Hydroxyapatite collagen bone graft and platelet rich plasma promote healing, induce bone formation and reduce bone resorption.

Wide alveolar cleft and midface distraction: Report of a case.

BACKGROUND: Cleft lip and palate patients often present wide alveolar cleft and midface hypoplasia. Closure of such wide alveolar clefts may be difficult using bone graft. Traditional orthognathic surgery for midface advancement shows relapse. Distraction osteogenesis (DO) as a modality for midface advancement has shown good results with external distractors. Use of internal distractor (ID) further improves patient compliance, causes minimal discomfort and offers the advantage of limiting relapse. The purpose of this study was to evaluate the versatility of intraoral distracters in midface advancement in cases with alveolar cleft. METHOD: A 16 years old young girl with midface deficiency and alveolar cleft visited our outpatient clinic for aesthetic improvement and midface distraction was planned. After Le Fort I osteotomy, internal distractor was fixed. A latency period of 5 days was allowed and then distraction was started at the rate of 1 mm per day in two installments. Evaluation was done for closure of cleft, ease of the procedure, stability, and complications. Lateral cephalograms were evaluated at three stages: predistraction; post-distraction; and 1 year post-distraction. RESULTS: Complete closure of alveolar cleft was observed with 17 mm midface advancement and bone formation at the pterygomaxillary region. Maxillary position improved in relation to the cranial base. The results were stable even at 1 year follow-up. CONCLUSION: Distraction osteogenesis using intraoral distractors was successful in alveolar cleft closure, as well as midface advancement in terms of stability of results and patient compliance with minimal complications.

Association of mitochondrial deoxyribonucleic acid mutation with polymorphism in CYP2E1 gene in oral carcinogenesis.

BACKGROUND: Oral carcinogenesis is a complex process affected by genetic as well as environmental factors. CYP2E1 gene is involved in metabolism of number of compounds and carcinogens. Its normal functioning is required for homeostasis of free radical. Mitochondrial deoxyribonucleic acid (mtDNA) is 10-100 times more susceptible to damage than nuclear DNA. Mitochondrial DNA large scale deletions are well documented in oral cancer. However, the relationship between CYP2E1 gene polymorphisms and mtDNA damage is still not documented in literature. MATERIALS AND METHODS: Case-control study involving 50 subjects was carried out. Deoxyribonucleic acid extraction was done from study subject tissue samples. Restriction fragment length polymorphism (RFLP) and polymerase chain reaction (PCR) amplification was done to confirm CYP2E1 gene polymorphisms. The PCR amplification was done for mtDNA 4977 bp deletion. Statistical analysis was carried out using SPSS version 11.5 with χ(2) tests. RESULTS: c1c1 and DD polymorphisms are prevalent in North Indian population having oral cancer. These polymorphisms are significantly associated with mtDNA 4977 bp deletion. CONCLUSION: Mitochondrial DNA damage induced by wild CYP2E1 forms and imperfect DNA repair in mtDNA may act synergistically to greatly enhance oral cancer risk.

Alveolar ridge augmentation using distraction osteogenesis: a clinical trial.

BACKGROUND: Severely resorbed ridges present a great challenge to prosthodontic rehabilitation. Available reconstructive options include autologous/alloplastic augmentation with questionable results, or regeneration of new bone under gradual and controlled tension using distraction osteogenesis. This study focused on use of distraction osteogenesis for the treatment of vertically deficient alveolar ridges to assess its feasibility and outcome. MATERIALS AND METHODS: Alveolar distraction osteogenesis (ADO) was studied at 10 different intra-oral, partial or complete edentulous sites. After a latency period of 5 days, distraction was carried out for 6-7 days at the rate of 0.5 mm every 12 hours (1 mm/day). Distractor was removed after a consolidation period of 12 weeks. RESULTS: The mean height gained at the 10 intra-oral sites was 4.8 mm with standard deviation of ± 0.056. The mean follow-up period was 2 years. Complications of therapy included hardware failure, wound gape and extra-oral scar. The overall complication rate was 10%. CONCLUSION: Distraction osteogenesis is a promising option to aid uneventful prosthodontic rehabilitation of severe vertically resorbed alveolar ridges.

Dimensional differences in mandibular antegonial notches in temporomandibular joint ankylosis.

BACKGROUND: Deep antegonial notch (AN) is seen in congenital and acquired abnormalities of mandible like condylar hypoplasia, temporomandibular joint ankylosis (TMA), muscular hypoactivity and brachial arch syndrome. This study was planned with an aim to study the depth of AN in TMA and find its relation with the duration of ankylosis. MATERIALS AND METHODS: The study comprised 20 cases of unilateral or bilateral TMA, with age range 8-25 years. A comparison between ipsilateral and contralateral AN was done on orthopantomograms of these bilateral and unilateral cases with the time period of total duration of ankylosis. RESULTS: Seven cases had right-sided ankylosis, six had left and seven had bilateral TMA where the history of ankylosis ranged from 6 months to 12 years. Spearman's rank correlation indicated a strong correlation between the duration of history of ankylosis and AN on the ipsilateral/contralateral sides. Wilcoxin signed rank test proved the results to be statistically significant. CONCLUSION: Deep accentuated AN is one of the clinical features of the TMA and has a direct relation to the morphology and growth pattern of mandible.