RESUMO
Analysis of clinical-roentgen-laboratory features of the course of hereditarily determined gout in 74 patients is represented. It is exhibited that hereditarily induced gout begins at the young age and is accompanied by the acute disturbance of purine exchange resulting in serious relapsing course of the articular (with availability of radiological attributes of osteal destruction) and the renal syndromes (urate nephrolithiasis, immune complex glomerulonephritis, tubulointerstitional nephritis) as well as in the early occurrence and rapidly progressing chronic renal insufficiency. The disease is accompanied by high frequency of the cardiovascular pathologies negatively influencing the mean lifetime of patients.
Assuntos
Predisposição Genética para Doença , Gota/genética , Adulto , Fatores Etários , Doenças Cardiovasculares/etiologia , Doenças Cardiovasculares/genética , Doenças Cardiovasculares/mortalidade , Transtornos Cerebrovasculares/etiologia , Transtornos Cerebrovasculares/genética , Transtornos Cerebrovasculares/mortalidade , Feminino , Gota/complicações , Gota/diagnóstico por imagem , Gota/mortalidade , Humanos , Masculino , Síndrome Nefrótica/etiologia , Síndrome Nefrótica/genética , Síndrome Nefrótica/mortalidade , Nucleotidases/metabolismo , Oxipurinol/metabolismo , Linhagem , Radiografia , Fatores Sexuais , Ácido Úrico/metabolismo , Xantina Oxidase/metabolismoRESUMO
Patients with system lupus erythematosus, rheumatic arthritis, chronic active hepatitis and gout were found to have considerable hyperuricemia and be decreased in aminoacides content, which are the predecessors of purine. Hyperactivity of xanthineoxidase and POL content were also revealed. The close correlation relation of purine indices of such patients has been observed. The obtained data allow applying methods of correction of immunity system and purine metabolism to these patients by introducing in a complex therapy inhibitors of xanthineoxidase.
Assuntos
Aminoácidos/sangue , Doenças Autoimunes/metabolismo , Gota/metabolismo , Hiperuricemia/metabolismo , Purinas/metabolismo , Aminoácidos/metabolismo , Doenças Autoimunes/sangue , Doenças Autoimunes/complicações , Gota/sangue , Gota/complicações , Humanos , Hiperuricemia/sangue , Hiperuricemia/etiologia , Peróxidos Lipídicos/sangue , Ácido Úrico/sangue , Xantina Oxidase/sangueRESUMO
Experimental autoimmune disease causes a drastic increase in the production of nitric oxide metabolites. The latter correlate with the indices of autoimmunity expression, with the levels of cyclic nucleotides, with the severity of morphological impairment of the structures of immunocompetent organs. The use of immunosuppressive agents in the pathogenetic therapy of an autoimmune process normalizes both the blood levels of nitrites/nitrates and immunological parameters; this reflects a clear-cut relationship between the synthesis of nitric oxide and the immune response of the body.
Assuntos
Doenças Autoimunes/tratamento farmacológico , Doenças Autoimunes/metabolismo , Imunossupressores/farmacologia , Óxido Nítrico/metabolismo , Animais , Doenças Autoimunes/imunologia , AMP Cíclico/sangue , GMP Cíclico/sangue , DNA/imunologia , Modelos Animais de Doenças , Feminino , Masculino , Nitratos/sangue , Óxido Nítrico/sangue , Nitritos/sangue , Ratos , Ratos Wistar , Baço/efeitos dos fármacos , Baço/imunologia , Baço/patologia , Timo/efeitos dos fármacos , Timo/patologiaRESUMO
The physical-and-chemical condition of condensate of breathed-out moisture (CBM) was studied by the computer-aided analysis of axis-symmetric drops' form in healthy subjects; such condition was found to depend on sex, age and smoking-addiction of the examined persons. The surface tension of the mentioned moisture correlated with its viscoelastic index and with relaxation. The metabolites of nitric oxide, lipids, urea, lactic acid (not uric acid) and of hydrogen peroxide were found to influence the physical-and-chemical parameters of respiratory moisture. Hydrogen peroxide had a reverse correlation with the CBM viscoelastic module. A status of the pulmonary surfactant can be evaluated by using the studied physical-and-chemical CBM parameters.
Assuntos
Peróxido de Hidrogênio/análise , Óxido Nítrico/análise , Surfactantes Pulmonares/química , Adulto , Fatores Etários , Testes Respiratórios/métodos , Feminino , Humanos , Masculino , Reologia , Fatores Sexuais , EspectrofotometriaRESUMO
The article presents a comparative analysis of methods for quantitative assessment of roengenologic changes in the joints in rheumatoid arthritis that have come to be widely used in USA and Europe. It is noted that the Steinbroker's method widely employed by clinicians fails to give an integral assessment of the articular affection. Expediency is documented of employment of the Sharp's method in roentgenologic monitoring in clinical trials of new antirheumatic drug preparations. In a routine clinical setting the Larsen's method or its modifications are to be preferred since in spite of its low sensitivity investigation time is cut down 2.3-fold by comparison with the Sharp's method.
Assuntos
Artrite Reumatoide/diagnóstico por imagem , Artrografia/métodos , Progressão da Doença , Humanos , Sensibilidade e EspecificidadeRESUMO
AIM: To study dynamic surface tension (DST) of blood and urine in SLE patients. MATERIALS AND METHODS: Clinical, immunological examinations, DST of blood and urine using computer tensiometers were made in 67 SLE patients aged 15 to 62 years. RESULTS: Changes in interphasic tensiograms in males and females were characterized regarding the course, activity of the pathological process and the presence of certain clinical symptoms. Effective therapy return to normal DST of the biological fluids. Correlations between interphasic tensiograms of blood, urine and levels of proteins, lipids, non-protein and inorganic surfactants and surface-inactive substances were established as well as relations of DST with biochemical composition of the biological fluids. CONCLUSION: The above diagnostic method may be useful for differential diagnosis, assessment of the clinical course, activity of the disease, control over effectiveness of the treatment.
Assuntos
Testes Hematológicos/métodos , Lúpus Eritematoso Sistêmico/sangue , Lúpus Eritematoso Sistêmico/urina , Urinálise/métodos , Adolescente , Adulto , Proteínas Sanguíneas/análise , Diagnóstico Diferencial , Feminino , Humanos , Lúpus Eritematoso Sistêmico/diagnóstico , Masculino , Pessoa de Meia-Idade , Tensão SuperficialRESUMO
Chronic dust bronchitis in miners is associated with increase in dynamic surface tension of blood at short (t = 0.01 s) and medium (t = 1 s) surface lifetimes with lower declination angle of interphase tensiograms curve. Surface tension disorders were similar to those in experimental dust bronchitis. There are correlations between surface tension parameters and the workers' underground length of service, some mining geologic conditions and serum level of proteous and lipid surfactants.
Assuntos
Bronquite/etiologia , Minas de Carvão , Poeira/efeitos adversos , Doenças Profissionais/etiologia , Adulto , Idoso , Doença Crônica , Hemodinâmica , Humanos , Masculino , Pessoa de Meia-IdadeRESUMO
AIM: To try polyenzyme drug vobenzim in chronic glomerulonephritis (CGN). MATERIALS AND METHODS: 174 CGN patients were randomized into 2 groups. The study group of 39 patients received conventional pathogenetic treatment plus vobenzim (initial dose 18-24, maintenance 12-15 dragees a day). Control group of 135 patients were given only pathogenetic therapy. 67 patients appeared ineligible. RESULTS: 3-6 months after the treatment the response was seen in 61.5% patients of the study and 17.8% patients of the control group. Side effects of vobenzim in CGN treatment were absent. CONCLUSION: Enzyme therapy with vobenzim in CGN is safe and effective.
Assuntos
Glomerulonefrite/tratamento farmacológico , Peptídeo Hidrolases/uso terapêutico , Administração Oral , Adolescente , Adulto , Idoso , Doença Crônica , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Peptídeo Hidrolases/administração & dosagem , Segurança , Resultado do TratamentoRESUMO
The disease was simulated by inhalation of dust containing 29% of free silica in Wistar male rats during 8 months. One group of the subjects was kept under 26-28 degrees C and other one--under 38-40 degrees C. Dynamic surface tension of lung tissue homogenate was studied by means of computer tensiometer MRT (Lauda, Germany). Interphase tensiometry precisely characterizes state of lung surfactant in experimental chronic dust bronchitis. Equilibrium surface tension and surface tension for 0.01 and 1 sec deteriorate during combined exposure to silica dust and heating microclimate. The studies demonstrated strong correlation between interphase tensiogram parameters and individual lipids content of lung surfactant. Surface tension is related to the state of lipids peroxidation in lungs and antioxidant systems.
Assuntos
Bronquite/fisiopatologia , Poeira/efeitos adversos , Surfactantes Pulmonares/fisiologia , Dióxido de Silício/efeitos adversos , Animais , Bronquite/etiologia , Doença Crônica , Modelos Animais de Doenças , Peroxidação de Lipídeos/fisiologia , Pulmão/fisiopatologia , Masculino , Ratos , Ratos Wistar , Tensão Superficial , TemperaturaRESUMO
An improved version of the MPT2 (LAUDA) computerized tensiometer (Germany) was used to measure dynamic surface tension (ST) of blood and urine by the maximum bubble pressure method in patients with tumors of the uterus, vagina and cervical canal. Relatively low blood and urine ST values at t-->infinity and serum at t = 1 s were registered. Combined radiotherapy involved oppositely-directed changes in the equilibrium (static) ST of the biological fluids as well as a urine ST inhibition in the surface short-life range (t = 0.01 s). The slope of blood serumtension curves by the end of treatment was close to that in healthy females.
Assuntos
Sangue , Neoplasias dos Genitais Femininos/fisiopatologia , Urina , Feminino , Neoplasias dos Genitais Femininos/radioterapia , Humanos , Tensão Superficial , Resultado do TratamentoRESUMO
Dynamic superficial tension (ST) of spinal fluid (SF) and blood serum in patients with CNS tumors was investigated. The maximum bubble tension was measured by a MPT2 computer tensiometer (LAUDA). There were decreases in ST of blood serum at t = 1 and t-->infinity, increases in ST at t = 0.01 and decreases in the angle on SF tensiograms. The parameters of interphasic tensiometry of the biological fluids depend on content of biologically active substances severity of the neurological symptomatology and duration of disease. Successive surgical treatment is followed by normalization of SF and blood serum ST.
Assuntos
Líquido Cefalorraquidiano/química , Neoplasias do Sistema Nervoso/sangue , Neoplasias do Sistema Nervoso/líquido cefalorraquidiano , Adolescente , Adulto , Idoso , Criança , Pré-Escolar , Feminino , Humanos , Masculino , Métodos , Pessoa de Meia-Idade , Tensão Superficial , Tensoativos/químicaRESUMO
Experiments on 24 Wistar male rats were performed to clarify the role of central mechanisms in the onset of electrical instability of the heart due to disturbances in purine metabolism. The activity of the enzymes responsible for synthesis and conversion of adenosine-5-nucleotidase and adenosine deaminase in the sensomotor cortex, hypothalamus and hippocamp was studied. Changes in cardiac function were most severe in high levels of adenosine in the neurons of the sensomotor cortex, hypothalamus and hippocamp. Minimal changes occurred in low content of adenosine in the hypothalamus and hippocamp.
Assuntos
Adenosina/metabolismo , Encéfalo/metabolismo , Coração/fisiologia , Ácido Úrico/sangue , Animais , Modelos Animais de Doenças , Masculino , Ratos , Ratos WistarRESUMO
The role of changes in biogenic amines neurochemistry in cerebral structures in abnormal immunoreactivity accompanying development of autoimmune disease was investigated in experiments on 24 Wistar male rats. Biogenic amines and their metabolites (dopamine, dioxyphenylacetic acid, serotonin, oxyindolacetic acid) were assays in the sensomotor cortex, hypothalamus and hippocamp by means of high-performance liquid chromatography. Development of adjuvant arthritis was associated with definite neurochemical changes in the brain: inhibition of serotonin metabolism and enhancement of dopaminergic systems in the hypothalamus and sensomotor cortex. Prophylactic administration of oil solution D,L-alpha-tocopherol acetate promoted enhancement of serotonin metabolism in hypothalamic and hippocampal neurons. Bitemporal exposure to UHF electric field aroused stronger effects on the neurochemical metabolism in hypothalamic, hippocampal and sensomotor cortex neurons than TPA. It is concluded that bitemporal exposure to UHF electric field and antioxidant activate central immunoreactivity mechanisms in animals with adjuvant arthritis. Serotonin and dopamine systems in this process are leading.
Assuntos
Artrite Experimental/metabolismo , Aminas Biogênicas/metabolismo , Encéfalo/metabolismo , Animais , Modelos Animais de Doenças , Masculino , Ratos , Ratos WistarRESUMO
AIM: Assessment of the dynamic surface tension (DST) of blood serum (BS), synovial fluid (SF) in various courses of rheumatoid arthritis (RA). MATERIALS AND METHODS: Forty three patients with RA and 63 apparently healthy individuals were examined. DST of BS and SF was determined in the computer-aided tensiometer and some blood biochemical parameters were also measured. RESULTS: DST of BS in patients with RA were found to be higher than the normal values and some parameters c beta 2 and beta 3) of BS DST did not significantly differ from the normal values whereas others (beta 1 and gamma proved to be much lower. Depending on the disease course variants, there were some differences in these DST changes. There was a feedback between DST parameters and the levels of immunoglobulins, beta 2-microglobulin, and lipids in blood. CONCLUSION: Assessment of BS and SF DST may be useful in the differential diagnosis of RA, in the determination of its intensity and prognosis, and therapeutical efficiency.
Assuntos
Artrite Reumatoide/fisiopatologia , Viscosidade Sanguínea/fisiologia , Líquido Sinovial/fisiologia , Adolescente , Adulto , Artrite Reumatoide/sangue , Artrite Reumatoide/diagnóstico , Diagnóstico Diferencial , Processamento Eletrônico de Dados , Feminino , Humanos , Lipídeos/sangue , Masculino , Pessoa de Meia-Idade , Prognóstico , Tensão Superficial , Microglobulina beta-2/metabolismoRESUMO
The condition was studied of the dynamic surface tension of biologic fluids in patients with rheumatoid arthritis using a method of maximum pressure in the vesicle with the aid of computerized tensiometer MPT-1 "Lauda" (Germany). Rise in indices of blood serum surface tension with diminution of the slope of tensiogram curves may suggest the development of pathology of the heart and liver, while increase in the same parameters of urine might be indicative of the coming nephropathy.
Assuntos
Artrite Reumatoide/fisiopatologia , Fenômenos Fisiológicos Sanguíneos , Urina/fisiologia , Vísceras , Adolescente , Adulto , Artrite Reumatoide/complicações , Feminino , Humanos , Inflamação/etiologia , Inflamação/fisiopatologia , Masculino , Pessoa de Meia-Idade , Análise Multivariada , Tensão SuperficialRESUMO
Dynamic surface-tension (S-T) was investigated of blood and urine of healthy individuals and patients with chronic glomerulonephritis by the proposed method of maximum pressure in a bubble with the aid of the computer-assisted device tensometer MPT-1 (Lauda, Germany). Patterns of changes in dynamic and static S-T were found out as were those of the slope of the curve in patients with mesangioproliferative and mesangiocapillary variants of the condition, in nephrotic syndrome and chronic renal insufficiency. Correlation type comparison was performed of physical-and-chemical properties of biological fluids to parameters characterizing protein and fat exchange. Relationship between dynamic S-T and biochemical composition of blood and urine was revealed. Diagnostic value of results of the studies made is discussed.
Assuntos
Glomerulonefrite/sangue , Glomerulonefrite/urina , Adolescente , Adulto , Idoso , Doença Crônica , Feminino , Humanos , Falência Renal Crônica/sangue , Falência Renal Crônica/urina , Masculino , Pessoa de Meia-Idade , Síndrome Nefrótica/sangue , Síndrome Nefrótica/urina , Tensão SuperficialAssuntos
Minas de Carvão , Artropatias/epidemiologia , Exposição Ocupacional , Adulto , Humanos , Masculino , Federação RussaRESUMO
Coal miners suffering from rheumatoid arthritis (RA), deforming osteoarthrosis (OA) and gouty arthritis (GA) were found to develop hypotestosteronemia in a study on the condition of the hypophysis-and-gonads system, this being observed even in essentially healthy individuals. With all arthronoses, androgen levels get depressed, particularly so in the presence of increased activity of lutropin. In RA examenees blood concentration of estradiol tends to increase, while OA and GA miners demonstrate reduced content of estrogen, with manifest hyperprogesteronemia being common. Hormonal dysbalance correlates with gravity of the course of the inflammatory-degenerative processes in the locomotor system.
Assuntos
Minas de Carvão , Estradiol/sangue , Hormônio Foliculoestimulante/sangue , Artropatias/sangue , Hormônio Luteinizante/sangue , Doenças Profissionais/sangue , Progesterona/sangue , Testosterona/sangue , Artrite Gotosa/sangue , Artrite Reumatoide/sangue , Humanos , Osteoartrite/sangue , UcrâniaRESUMO
The studies covered serum levels of total and ionized calcium (Ca), parathyroid hormone (PTH), calcitriol (Ctr) and calcitonin (Ct) in miners assigned to various occupational groups and suffering from deforming osteoarthrosis. Ratio of calcium clearance to glomerular filtration rate was assessed. Ionized Ca appeared to decrease along with increase of PTH and Ctr. Changes in Ca homeostasis were proved to depend on occupation, geologic conditions in the mines (depth, ambient temperature of the workplace), presence of chondrocalcinosis.
Assuntos
Cálcio/metabolismo , Mineração , Osteoartrite/metabolismo , Adulto , Calcitonina/sangue , Calcitriol/sangue , Cálcio/sangue , Taxa de Filtração Glomerular , Homeostase , Humanos , Masculino , Osteoartrite/sangue , Hormônio Paratireóideo/sangueRESUMO
An immunologic evaluation was done in healthy miners and those working in coal mines suffering from deforming osteoarthrosis. Blood levels of beta 2-microglobulin and circulating immune complexes, titres of antibodies to glycosaminoglycans and antigens from cartilaginous tissue and synovial membrane were all found to be elevated. Shifts in the immunity system were clearly related to the spread of the pathological process as well as the rate of the disease progression and presence of reactive synovitis but not with occupation of the patients. Clinico-immunologic manifestations of osteoarthrosis were unfavourably influenced by industrial mining-geological factors.