RESUMO
The characterization of the tonoplast intrinsic protein ZmTIP2-3 cDNA isolated from maize roots is reported. ZmTIP2-3 belongs to the TIP2 group according to the present nomenclature. The aquaporin function of ZmTIP2-3 protein was demonstrated using expression in X. laevis oocytes. Northern blot analyses revealed that ZmTIP2-3 was specifically expressed in roots. Salt and water stresses induced the accumulation of ZmTIP2-3 transcripts. By contrast, no effect of ABA was observed. An oscillation of ZmTIP2-3 transcript amount during the day-night cycle was observed with some typical features of genes regulated by a circadian mechanism.
Assuntos
Aquaporinas/genética , Proteínas de Plantas/genética , Zea mays/genética , Sequência de Bases , Primers do DNA , Regulação da Expressão Gênica de Plantas , RNA de Plantas/genética , RNA Ribossômico 18S/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Transcrição GênicaRESUMO
In maize (Zea mays) roots, xylem water transfer supported by root pressure occurs during the day and is less important at night. Diurnal modifications of osmotic pressure gradient between medium and xylem could not explain the oscillation of water flux in young maize roots during the day-night cycle. We observed a high turgor pressure of root cortical cells associated with a high flux. In maize roots, ZmPIP transcripts oscillate during the day-night cycle exhibiting some characteristics of genes regulated by a circadian mechanism. The PIP protein level profile is different from the mRNA pattern. Moreover, ZmPIP1 and ZmPIP2 protein levels are differentially regulated during the light and dark period and in response to continuous darkness suggesting different roles for both classes of PIP. Finally, our results suggest that aquaporins from ZmPIP2 subgroup may contribute to root water transfer by cellular pathway that occurs during the light and the dark period of the day-night cycle.
