[Monoclonal antibodies IGR to surface antigens of neutrophilic granulocytes in human blood]. / Monoklonal'nye antitela IGR k poverkhnostym antigenam neitrofil'nykh granulotsitov krovi cheloveka.

Four monoclonal antibodies (MAbs) from series IGR to human peripheral blood neutrophilic granulocyte cell surface antigens were obtained by the conventional hybridoma technique. Specificity of MAbs AGR was determined to various leukemic cell lines and human peripheral blood cells. Overlapping in characteristics of antigens (molecular weight, localization, expression on induced leukemic cell line HL-60) to MAbs IGR-1 4C7, IGR-1 5B6 and IGR-2 IA6 suggests their identity. These, apparently, cannot be analogous to the well known granulocyte cell surface glycoproteins LFA-1, CR-3, p150, 95 or GP 130. The characteristics of MAbs IGR-1 and IGR-2 permit concluding that the antibodies should be useful in normal and leukemic myelomonocytic cell linear differentiation studies.

[Monoclonal antibodies IGR to nuclear antigens of neutrophilic granulocytes of human blood]. / Monoklonal'nye antitela IGR k iadernym antigenam neitrofil'nykh granulotsitov krovi cheloveka.

Four monoclonal antibodies (Mabs) of series IGR-1 and IGR-2 to nuclear antigens of neutrophilic granulocytes of human peripheral blood were obtained. Mabs IGR-1 2B8 and IGR-1 6B5 are bound to their specific antigens in the nuclei of all the investigated human cell lines. These Mabs were also specific for metaphase chromosomes of cell lines HL-60 and U-937. Investigations on the ultrastructural level showed that Mabs IGR-1 6B5 reacted with the HL-60 nuclear heterochromatin region. Mabs IGR-1 3D3 and IGR-2 2F1 manifested high specificity only for the nuclei of mature neutrophils and of plasma cells.