The development of ZnO nanoparticle-embedded graphitic-carbon nitride towards triple-negative breast cancer therapy.

The present study deals with the effects of curcumin-loaded ZnO nanoparticles (NPs) embedded in graphitic-carbon nitride (g-C3N4) sheets for breast cancer cells. The synthesis of these sheets was carried out by a simple co-precipitation method. The physicochemical and thermal properties of the composite sheets were studied using various characterization techniques. The powder X-ray diffraction and high-resolution transmission electron microscopy analyses confirmed the hexagonal wurtzite phase of the ZnO nanoparticles, which were randomly distributed on the g-C3N4 nanosheets, generating a finely bonded interface between the two components. The X-ray photoelectron spectroscopy analysis confirmed the successful formation of the g-C3N4@ZnO composite, while the thermal studies revealed the thermal stability of the composite. In addition, the drug release and kinetics studies proved that the release of curcumin was more significant under acidic conditions (pH 5) compared with neutral pH (7.4). Further, the biological assays verified the antibacterial activity (against two different cultures of E. coli and S. aureus) and anticancer activity (against MDA-MB-231 cancer cells) of the g-C3N4@ZnO/C nanocomposite. Finally, the lactate dehydrogenase activity assay presented the cytotoxic assessment of the nanocomposite based on its cytoplasmic activity and the extent of enzymes released from the damaged cells.

Biosynthesised Silver Nanoparticles Loading onto Biphasic Calcium Phosphate for Antibacterial and Bone Tissue Engineering Applications.

Biphasic calcium phosphate (BCP) serves as one of the substitutes for bone as it consists of an intimate mixture of beta-tricalcium phosphate (ß-TCP) and hydroxyapatite (HAP) in different ratios. BCP, because of its inbuilt properties such as osteoconductivity, biocompatibility, and biostability in several clinical models serves as a bone substituent for orthopedic applications. Therefore, the present study aimed to assess the effectiveness of silver (Ag) nanoparticles (NPs) combined with BCP composites for the orthopedic sector of bone tissue regeneration and growth. In this regard, we first synthesized Ag-BCP microclusters by the double-emulsion method and then characterized the composite for various physicochemical properties, including the crystallinity and crystal structure, bonding and functionality, porosity, morphology, surface charges, topography, and thermal stability. In addition, the antibacterial activity of Ag-BCP was tested against gram-positive and gram-negative microorganisms such as Staphylococcus aureus, Candida albicans, and Escherichia coli. Finally, the cytocompatibility of Ag-BCP was confirmed against the fibroblast cells in vitro.