1.
Chem Commun (Camb)
; 53(43): 5858-5861, 2017 May 30.
Artigo
em Inglês
| MEDLINE
| ID: mdl-28504793
RESUMO
We describe an approach to generating and verifying well-defined redox states in metalloprotein single crystals by combining electrochemical control with synchrotron infrared microspectroscopic imaging. For NiFe hydrogenase 1 from Escherichia coli we demonstrate fully reversible and uniform electrochemical reduction from the oxidised inactive to the fully reduced state, and temporally resolve steps during this reduction.