Epidemiological Study of Parasitic Infections in BuMusa Island, Hormozgan.

BACKGROUND: This epidemiological study aimed to investigate the prevalence of parasitic infections in BuMusa Island, Iran, in one year from 2015 to 2016. METHODS: The current cross-sectional study was conducted in coordination with the health authorities of BuMusa on 732 intestinal samples and 1207 blood samples randomly collected from the island residents. Cutaneous lesions of 1207 people were clinically examined and those suspected of parasitic infections were enrolled. Also, 165 intestinal samples from domestic animals, 35 samples from water tanks, and 330 soil samples were taken to the laboratory to be investigated in terms of parasitic infections. RESULTS: The obtained results showed 26.4% and 45.5% intestinal parasitic infections in humans and animals, respectively. The most prevalent infections in humans were Blastocystis hominis (8.6%), followed by Giardia lamblia (8.2%), and Entamoeba coli (6.8%); and the least prevalent infection was Enterobius vermicularis (<0.2%). Malaria agents and Leishmania were not observed in blood samples. Investigation of animal feces showed that the highest parasitic infection was Eimeria arloingi (16.4%), while the lowest prevalence belonged to Monizia expansa (0.6%). Hymenolepis nana eggs and Cyclops were detected in one sub-source of water tanks. Rhabditis larva, a free-living nematode, was observed in a soil sample. CONCLUSION: The prevalence of parasitic infections in BuMusa Island was relatively low probably due to its hot and dry climate.

Chemiluminescent microparticle immunoassay-based detection and prevalence of Toxoplasma gondii infection in childbearing women (Iran).

Toxoplasmosis is a common zoonotic disease, which is of particular importance in women before and during pregnancy. In this study, the chemiluminescent microparticle immunoassay was used to detect Toxoplasma gondii and the seroepidemiology of Toxoplasma gondii was investigated among childbearing women in Qom, Iran. In this study, 202 blood samples were collected from childbearing women for evaluation of toxoplasmosis. Serum samples were isolated to determine anti-Toxoplasma IgM and IgG antibodies. Based on findings, the total prevalence of anti-Toxoplasma antibodies was 134 (66.3%) [95% CI 59.6-72.5]. In addition, 133 (65.8%) [95% CI 59.1-72.1], 19 (9.4%) [95% CI 6.1-14.2] and 18 (8.9%) [95% CI 5.7-13.6] samples were IgG-positive, IgM-positive and positive for both antibodies, respectively. The highest infection rate was reported over the age of 35 years, and positive lgM antibodies were detected in women below 29 years. T. gondii infection showed a significant correlation with age, pregnancy, contact with cats, exposure to soil, and consumption of raw food (P < 0.05). The serological tests revealed that nearly 33.7% [95% CI 27.5-40.4] of women at childbearing age (49.1% [95% CI 36.4-61.9] of pregnant women versus 27.9% [95% CI 21.3-35.6] of non-pregnant women) had no anti-Toxoplasma antibodies. Therefore, this population is more prone to acute infections with Toxoplasma.

Species diversity of mosquitoes of the Genus Culex (Diptera, Culicidae) in the coastal areas of the Persian Gulf.

BACKGROUND: Lack of information about the dispersal of vector species barricade surveillance and control. AIMS: Therefore, the aim of the present study was to determine the species diversity of Culex mosquito's larvae in the coastal areas of the Persian Gulf. METHODS: Mosquito larvae were collected from six places in three main environmental categories: urban (UA), rural (RA) and uninhabited areas (UNA), using dipping technique. Four dips were taken from each breeding site (350 ml each). Larval investigation was conducted two times a month during the study period. Diversity studies were conducted separately for each category by calculating classic diversity indices. RESULTS: In total, 1369 specimens belonging to 10 different species were collected and identified, as follows: Culex hortensis, Cx. laticinctus, Cx. mimeticus, Cx. perexiguus, Cx. pipiens, Cx. modestus, Cx. sinaiticus, Cx. theileri, Cx. torrentium and Cx. tritaeniorhynchus. None of these mosquito species have been recorded previously in this region. Diversity analysis indicated higher species richness for RA (Margalef 1/26). The average diversity indices for the three environment types ranged from 1.50 to 1.64 for Shannon index and from 0.730 to 0.738 for Simpson index. CONCLUSIONS: Biodiversity analysis indicated that species diversity in rural, urban and uninhabited areas is somewhat similar. Therefore, attention to all areas in vector control programs is essential.

Natural host preferences of parasitoid wasps (Hymenoptera: Pteromalidae) on synanthropic flies.

Synanthropic flies are members of order Diptera and considered as medical and veterinary pests. In this study, parasitoid wasps were determined and their natural host preferences in order to select a suitable agent for biological control of flies. The pupae of three species of flies; Musca domestica, Lucilia sericata and Sarcophaga haemorrhoidalis were used as hosts for natural parasitoids. For this issue, as much as 50 pupae of each fly species put in three separate dishes with covered top by a net. These dishes were placed in the field to attract parasitoid wasps. The most parasitic rate was related to N. vitripennis (%17.2). Host preferences of N. vitripennis on M. domestica pupae were higher than observed parasitism on L. sericata and S. haemorrhoidalis. The emerging rate of two parasitoids; P. vindemmiae and S. nigroaenea were one per host pupae. According to the result, N. vitripennis can be an appropriate candidate for use as natural enemy which expected to be effective in controlling various species of synanthropic flies. Therefore, S. nigroaenea was more suitable to biological control of housefly populations.

Anticancer and antibacterial effects of Iranian viper (Vipera latifii) venom; an in-vitro study.

Viper venom contains antibacterial and cytotoxic components. The aim of this study was to identify and evaluate the antimicrobial and cytotoxic properties of the crude venom of Vipera latifii (V. latifii). Lyophilized venom of V. latifii was quantified by Bradford method and its antibacterial activity (6.25-400 µg/ml) was assessed using the MTT, MIC, Disc diffusion, and Well diffusion assays. Also, its cytotoxic activity was investigated using MTT reduction, Neutral uptake, and Comet assay on human liver cancer (HepG2) cell line. Crude venom showed antibacterial effects against Bacillus subtilis and Staphylococcus aureus, but was not effective on Escherichia coli. Also, the crude venom showed apoptotic and necrotic effects on human liver cancer cells. The venom of V. latifii can inhibit the growth of bacteria and cancer cells. These findings suggest that this may be a potential source of molecules with antibacterial and anticancer characteristics.