High-Density Polyethylene Custom Focusing Lenses for High-Resolution Transient Terahertz Biomedical Imaging Sensors.

Transient terahertz time-domain spectroscopy (THz-TDS) imaging has emerged as a novel non-ionizing and noninvasive biomedical imaging modality, designed for the detection and characterization of a variety of tissue malignancies due to their high signal-to-noise ratio and submillimeter resolution. We report our design of a pair of aspheric focusing lenses using a commercially available lens-design software that resulted in about 200 × 200-µm2 focal spot size corresponding to the 1-THz frequency. The lenses are made of high-density polyethylene (HDPE) obtained using a lathe fabrication and are integrated into a THz-TDS system that includes low-temperature GaAs photoconductive antennae as both a THz emitter and detector. The system is used to generate high-resolution, two-dimensional (2D) images of formalin-fixed, paraffin-embedded murine pancreas tissue blocks. The performance of these focusing lenses is compared to the older system based on a pair of short-focal-length, hemispherical polytetrafluoroethylene (TeflonTM) lenses and is characterized using THz-domain measurements, resulting in 2D maps of the tissue refractive index and absorption coefficient as imaging markers. For a quantitative evaluation of the lens effect on the image resolution, we formulated a lateral resolution parameter, R2080, defined as the distance required for a 20-80% transition of the imaging marker from the bare paraffin region to the tissue region in the same image frame. The R2080 parameter clearly demonstrates the advantage of the HDPE lenses over TeflonTM lenses. The lens-design approach presented here can be successfully implemented in other THz-TDS setups with known THz emitter and detector specifications.

Development of Terahertz Imaging Markers for Pancreatic Ductal Adenocarcinoma using Maximum A Posteriori Probability (MAP) Estimation.

Pancreatic ductal adenocarcinoma (PDAC) is one of the significant reasons for cancer-related death in the United States due to a lack of timely prognosis and the poor efficacy of the standard treatment protocol. Immunotherapy-based neoadjuvant therapy, such as stereotactic body radiotherapy (SBRT), has shown promising results compared to conventional radiotherapy in strengthening the antitumor response in PDAC. To probe and quantify the antitumor response with SBRT, we propose to study the tumor microenvironment using terahertz time-domain spectroscopy (THz-TDS). Since the tumor's complex microenvironment plays a key role in disease progression and treatment supervision, THz-TDS can be a revolutionary tool to help in treatment planning by probing the changes in the tissue microenvironment. This paper presents THz-TDS of paraffin-embedded PDAC samples utilizing a clinically relevant genetically engineered mouse model. This Article aims to develop and validate a novel time-domain approximation method based on maximum a posteriori probability (MAP) estimation to extract terahertz parameters, namely, the refractive index and the absorption coefficient, from THz-TDS. Unlike the standard frequency-domain (FD) analysis, the parameters extracted from MAP construct better-conserved tissue parameters estimates, since the FD optimization often incorporates errors due to numerical instabilities during phase unwrapping, especially when propagating in lossy media. The THz-range index of refraction extracted from MAP and absorption coefficient parameters report a statistically significant distinction between PDAC tissue regions and their healthy equivalents. The coefficient of variation of the refractive index extracted by MAP is one order of magnitude lower compared to the one extracted from FD analysis. The index of refraction and absorption coefficient extracted from the MAP are suggested as the best imaging markers to reconstruct THz images of biological tissues to reflect their physical properties accurately and reproducibly. The obtained THz scans were validated using standard histopathology.

(Cd,Mg)Te crystals for picosecond-response optical-to-x-ray radiation detectors.

We demonstrate a photodetector sensitive to both optical and x-ray picosecond pulses based on our in-house grown cadmium magnesium telluride (Cd,Mg)Te single crystal. Specifically, we developed In-doped Cd0.96Mg0.04Te material and discuss its femtosecond optical photoresponse, as well as the detector performance, such as <100-pA dark current and up to 0.22-mA/W responsivity for 780-nm wavelength optical radiation. The detector exposed to Ti fluorescence (K alpha) x-ray pulses at 4.5 keV, generated by a free-electron laser beam with the central energy of 9.8 keV and <100 fs pulse width, exhibited readout-electronics-limited 200-ps full-width-at-half-maximum photoresponse, demonstrating that it is suitable for coarse timing in free-electron laser x-ray/optical femtosecond pump-probe spectroscopy applications.

Evaluation of nonunions of the tibia diaphysis - own experience.

BACKGROUND: Delayed union is not an expected outcome of treatment of a bone fracture. The cause of the delayed union must be established before treatment of the complication can be undertaken. The method of treatment depends mainly on the specific nature of delayed bone union. The study evaluates the causes of delayed bone union during treatment of tibial shaft fractures. MATERIAL AND METHODS: Seventy-two patients with delayed union following a fracture of the tibial diaphysis were treated in the Department of Orthopaedics and Musculoskeletal Traumatology of Wroclaw Medical University between 1995 and 2005. All patients had received previous treatment of the fractures. Depending on the morphology of the delayed union, the treatment was with Ilizarov's compression-distraction method or autologous osteoplasty with intramedullary, internal or external fixation. The choice of a method was based on an analysis of the features of the non-union while fracture morphology, presence of other concomitant sequelae of the original injury, fixation method and possible surgical errors, post-operative patient management, co-morbidity and patient compliance were evaluated as risk factors of delayed bone union. The risk factors were subdivided into treatment-dependent (external) and treatment-independent (internal). Treatment outcomes were evaluated on the basis of an orthopaedic examination and radiographic evidence. RESULTS: In the vast majority of patients, delayed union of the tibial diaphysis was due to external factors. CONCLUSIONS: Delayed union of the tibia can be avoided through early and simultaneous treatment of all sequelae of the original trauma. Patient management must be based on state-of-the-art medical knowledge.

Characterization of single and double fiber-coupled diffusing spheres.

A fiber-optic sensor, consisting of an optical fiber with and without a 1.59-mm-diameter spherical ceramic tip, inserted into a 19-mm-diameter Spectralon sphere has been characterized. This sensor is evaluated as a large-area omnidirectional sensor. An optical transport measurement system that rotates the sphere about two axes has been designed. The system measured the UV transport efficiency at 351 nm of light impinging on the sphere with an f-6 cone angle. When a bare fiber was placed at the center of the target sphere, the detection sensitivity was biased in the forward direction. The peak of the sensitivity of the inverse integrating sphere shifted from front to back as the bare fiber was withdrawn from the sphere and the numerical aperture of the fiber viewed more of the scattering volume. The response function with respect to the angle of incidence of the dual sphere was much more uniform than that obtained with a bare fiber in the center of the sphere.

Time-resolved photoexcitation of the superconducting two-gap state in MgB2 thin films.

Femtosecond pump-probe studies show that carrier dynamics in MgB2 films is governed by the sub-ps electron-phonon (e-ph) relaxation present at all temperatures, the few-ps e-ph process well pronounced below 70 K, and the sub-ns superconducting relaxation below T(c). The amplitude of the superconducting component versus temperature follows the superposition of the isotropic dirty gap and the three-dimensional pi gap dependences, closing at two different T(c) values. The time constant of the few-ps relaxation exhibits a double divergence at temperatures corresponding to the T(c)'s of the two gaps.

Femtosecond response of a free-standing LT-GaAs photoconductive switch.

We present a novel, free-standing low-temperature GaAs (LT-GaAs) photoconductive switch and demonstrate its femtosecond performance. A 1-microm-thick layer of a single-crystal LT-GaAs was patterned into 5-10-microm-wide and 15-30-microm-long bars, separated from their GaAs substrate and, subsequently, placed across gold coplanar transmission lines deposited on a Si substrate, forming a photoconductive switch. The switch was excited with 110-fs-wide optical pulses, and its photoresponse was measured with an electro-optic sampling system. Using 810-nm optical radiation, we recorded an electrical transient as short as 360 fs (1.25 THz, 3-dB bandwidth) and established that the photo-carrier lifetime in our LT-GaAs was 150 fs. Our free-standing devices exhibited quantum efficiency of the order of approximately 7%, and their photoresponse amplitude was a linear function of the applied voltage bias, as well as a linear function of the excitation power, below a well-defined saturation threshold.