Assuntos
Amiloide/biossíntese , Células-Tronco Hematopoéticas/metabolismo , Macrófagos/metabolismo , Mieloma Múltiplo/patologia , Idoso , Amiloidose/patologia , Células Cultivadas , Imunofluorescência , Humanos , Imunoglobulina G , Masculino , Microscopia Eletrônica , Mieloma Múltiplo/tratamento farmacológico , Mieloma Múltiplo/metabolismoRESUMO
One-hundred-eighty-six previously untreated patients with malignant lymphoma were evaluated for immunocompetence by means of several tests of immune function: total circulating lymphocytes, T cells (E-rosettes), B cells (EAC-rosettes), delayed hypersensitivity to six recall antigens, serum immunoglobulins, mixed lymphocyte culture, and lymphocyte mitogenic response to phytohemagglutinin and pokeweed mitogen. The results were correlated with histology, stage, and clinical features. Diffuse lymphomas, especially diffuse histiocytic (large cell) (DHL), were associated with decreased absolute lymphocytes and E-rosette forming cells. Skin test reactivity varied with both histology and stage. For example, only one of six tests was impaired in diffuse lymphocytic well differentiated (DLWD) lymphoma in contrast to two of six in localized DHL and five of six in advanced DHL. Patients with nodular lymphoma exhibited depressed mean levels of IgA and IgG, while only IgA was significantly decreased in diffuse lymphoma. Mitogen stimulation was depressed in all groups, although mixed lymphocyte cultures did not differ significantly from controls. In summary, there is a spectrum of immunodeficiency of both B and T cell type in patients with malignant lymphoma that correlated with histology and stage. Implications and possible mechanisms of these observations are discussed.
Assuntos
Linfócitos B/imunologia , Linfoma/imunologia , Linfócitos T/imunologia , Adulto , Idoso , Humanos , Contagem de Leucócitos , Teste de Cultura Mista de Linfócitos , Linfoma/patologia , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Fito-Hemaglutininas/farmacologia , Formação de Roseta , Testes CutâneosRESUMO
An in vitro assay for clonogenic tumor cells was applied to human metastatic melanomas. Melanoma colony formation was observed in 22 of 33 samples obtained from a variety of involved tissues. The melanocytic tumor origin of colonies was established by serial observations by inverted light microscopy, staining of fixed colonies for melanin, and cytological and karyotypic analysis of cells within colonies. Two morphologically distinct types of colonies were identified, one consisting of light large cells and the second of dark small cells. Investigations of factors which modulate growth and differentiation of clonogenic melanoma cells may provide a clearer understanding of this neoplasm.
