Assuntos
Ferro/sangue , Talassemia/sangue , Adulto , Feminino , Heterozigoto , Humanos , Masculino , Pessoa de Meia-Idade , Talassemia/genética , Talassemia/terapiaRESUMO
This study concerns the comparison of two methodologies for reticulocyte count on two groups of 100 subjects. The evaluation of the results achieved with the two colorants (brilliant cresyl blue and pre-colored glasses with crystal acetate violet and methylene blue) suggests the application of more reliable parameters (reticulocyte index, reticulocyte production index) for the interpretation of reticulocyte counting. It seems desirable to correct the absolute reticulocyte number with the degree of anemia of the patient and to calculate the medullary production index to identify the effective reticulocytosis.
Assuntos
Contagem de Eritrócitos/métodos , Reticulócitos/citologia , Anemia/sangue , Diferenciação Celular , Feminino , Humanos , Masculino , Azul de Metileno , OxazinasAssuntos
Anticorpos Antivirais/análise , Biopterinas/análogos & derivados , HIV/imunologia , Interferon gama/sangue , Transtornos Relacionados ao Uso de Substâncias/imunologia , Complexo Relacionado com a AIDS/etiologia , Biopterinas/sangue , Proteínas do Sistema Complemento/análise , Feminino , Humanos , Imunoglobulinas/análise , Masculino , Neopterina , Risco , Microglobulina beta-2/análiseRESUMO
Description of an analytical procedure in order to show the atypical-CK carriers that simulate an abnormal raising of CK-MB circulating levels in dosages for immune inhibition. This procedure was applied to two samples taken from patients of such a type, immune form neoplastic and cardiac pathology. A systematic dosage of a "real" MB isoenzyme is desirable in all the cases of very raised CK-MB activity.
Assuntos
Ensaios Enzimáticos Clínicos/métodos , Creatina Quinase/sangue , Adulto , Idoso , Anticorpos/análise , Complexo Antígeno-Anticorpo/análise , Cromatografia por Troca Iônica/métodos , Creatina Quinase/imunologia , Eletroforese em Acetato de Celulose/métodos , Feminino , Humanos , Isoenzimas , Masculino , Pessoa de Meia-Idade , Infarto do Miocárdio/diagnósticoRESUMO
Here is underlined the role of quantitative determination of streptococcus A polysaccharide C antibody for the diagnosis of streptococcal infection. The Authors suggest to associate this determination with antistreptolysin titer and/or bacterial esoenzyme antibody titer, in order to achieve a wider spectrum of streptococcus induced antibodies for therapeutic and prophylactic applications.
Assuntos
Anticorpos Antibacterianos/análise , Polissacarídeos Bacterianos/imunologia , Infecções Estreptocócicas/diagnóstico , Streptococcus pyogenes/imunologia , Humanos , Estreptolisinas/imunologiaRESUMO
The results of a screening for beta-heterozygous thalassemia conducted on 999 school-boys aged from 11 to 13 are the following: 7.5% of the subjects were taker of the thalassemia trait; 74 subjects over 75 with beta-heterozygous thalassemia had "Mean Corpuscolar Volume" (MCV) values below 70 fl. The results of the subjects with globular volume less than or equal to 79 fl (with or without beta-thalassemia) were used to compare the diagnostic accuracy of beta-thalassemia for the hematological indexes of England-Fraser, Mentzer, Shine-Lal and the MCV estimations. This comparison has shown better results in terms of sensibility and specificity for MCV with respect to the other indexes. A more extensive application of the study could be convenient in order to evaluate if the MCV level of 70 fl is effective to discriminate between beta-thalassemia and non thalassemic microcytosis.