[Comparative sub-population analysis of exosomes from blood plasma of cancer patients]. / Sravnitel'nyi subpopuliatsionnyi analiz ékzosom plazmy krovi bol'nykh zlokachestvennymi novoobrazovaniiami.

To increase the sensitivity and specificity of the developed methods for diagnosis of oncological diseases using exosomes of blood, a stage of pre-selection of tumor exosomes from a common pool of circulating microvesicles is required. In the present work, universal proteins have been identified, their expression has been increased in the exosomes of patients with colorectal cancer, head and neck squamous cell carcinomas, and lung cancer. The use of antibodies against major exosomal proteins will further develop a simple and high-performance method of affinity isolation of tumor exosomes.

[Isolation and characterization of exosomes from blood plasma of breast cancer and colorectal cancer patients]. / Vydelenie i kharakterizatsiia ékzosom plazmy krovi bol'nykh rakom molochnoi zhelezy i kolorektal'nym rakom.

A simple approach for isolation of exosomes from the blood plasma, which allows to obtain highly purified preparations of microvesicles no larger than 100 nm has been proposed. The presence of different subpopulations of exosomes in the blood plasma of healthy donors and cancer patients has been recognized. We found the presence of the universal markers CD9, CD24 and CD81 on exosomes isolated from blood plasma that can be used to their routine typing.