RESUMO
AIM: To study changes in expression of TLR9, NF-kappaB, TNFalpha genes in cervical canal mucosa in pregnant women with herpesvirus infection. MATERIALS AND METHODS: Using previously developed real-time polymerase chain reaction assays, levels of expression of genes, which corresponding proteins play important role in TLR9-dependent signaling pathway, was quantified. RESULTS: Two-fold, 4.4-fold, and 3-fold rise in expression of TLR9, NF-kappaB, and TNFalpha genes respectively was revealed in pregnant women with herpesvirus infection compared to women with physiologically occurring pregnancy. CONCLUSION: Obtained results demonstrated the activation of TLR-mediated mechanisms of innate immunity on the level of cervical canal mucosa during pregnancy complicated by viral infection.
Assuntos
Colo do Útero/imunologia , Infecções por Herpesviridae/imunologia , NF-kappa B/genética , Complicações Infecciosas na Gravidez/imunologia , Receptor Toll-Like 9/genética , Fator de Necrose Tumoral alfa/genética , Adulto , Colo do Útero/virologia , Feminino , Expressão Gênica , Infecções por Herpesviridae/genética , Humanos , Mucosa/imunologia , Mucosa/virologia , Gravidez , Complicações Infecciosas na Gravidez/genética , Complicações Infecciosas na Gravidez/virologia , Regulação para CimaRESUMO
Innate immunity is the first line of host defense against infection including those caused by herpesviruses. Toll-like receptors (TLRs) are the main pathogen-recognizing receptors. TLR2 binds to glycoproteins of herpesvirus virion; intracellular TLR9 recognizes nonmethylated CpG repeats of herpesvirus' DNA, which lead to activation of receptor signaling pathways and synthesis of proinflammatory cytokines, chemokines, antimicrobial peptides and other molecules, which participate in induction of innate immunity mechanisms. Many viruses (measles virus, cytomegalovirus etc.) activate expression of TLRs for more effective replication of the virus in cells.