Repeated bolus injections of bupivacaine for continuous bilateral transversus thoracis plane block undergoing median sternotomy in a dog: A case report.

An 8-year-old, 6.5 kg, neutered female Shih-Tzu dog was presented for surgical resection of a mediastinal mass. A median sternotomy and left cranial lung lobectomy were performed. Intraoperatively, with the patient under general anaesthesia, a bilateral transversus thoracis plane (TTP) block was performed by injecting 0.5% bupivacaine (0.2 ml/kg) per side using real-time ultrasound guidance. After surgery, indwelling catheters for repeated bolus injections of bupivacaine in TTP were placed as follows: the fifth sternebra was palpated in dorsal recumbency, and the transducer was placed in the longitudinal plane lateral to the sternal border. A 16 gauge over-the-needle catheter was inserted caudo-cranially using an in-plane technique and located in the TTP. An intermittent bolus of bupivacaine (0.1 ml/kg) per side was injected via the indwelling catheter every 8 h for 3 days, with a constant rate infusion of an intravenous fentanyl (1 µg/kg/h) and ketamine (0.12 mg/kg/h) combination. Post-operative pain was evaluated using the Glasgow composite measure pain scale and the score was 4-5/24 on the day of surgery and gradually decreased over time. Additional rescue analgesia was not required. Repeated boluses of bupivacaine for a continuous bilateral TTP block may be a useful adjuvant for perioperative pain management strategies, including median sternotomy, in dogs.

Evaluation of a novel, low-cost, 3D printed video laryngoscope with borescope in anesthetized Beagle dogs.

OBJECTIVE: To develop and evaluate a low-cost three-dimensional (3D)-printed video laryngoscope (VLVET) for use with a commercial borescope. STUDY DESIGN: Instrument development and pilot study. ANIMALS: A total of six adult male Beagle dogs. METHODS: The VLVET consisted of a laryngoscope handle and a Miller-type blade, and a detachable camera holder that attached to various locations along the blade. The laryngoscope and camera holder were 3D-printed using black polylactic acid filament. Dogs were premedicated with intravenous (IV) medetomidine (15 µg kg-1) and anesthesia induced with IV alfaxalone (1.5 mg kg-1). The VLVET, combined with a borescope, was used for laryngeal visualization and intubation. Performance was evaluated by comparing direct and video-assisted views in sternal recumbency. The borescope camera was sequentially positioned at 2, 4, 6, 8 and 10 cm from the blade tip (distanceLARYNX-CAM), which was placed on the epiglottis during intubation or laryngoscopy. At the 10 cm distanceLARYNX-CAM, laryngeal visualization was sequentially scored at inter-incisor gaps of 10, 8, 6, 4 and 2 cm. Laryngeal visualization scores (0-3 range, with 0 = obstructed and 3 = unobstructed views) were statistically analyzed using the Friedman's test. RESULTS: Under direct visualization, the 2 cm distanceLARYNX-CAM had a significantly lower score compared with all other distanceLARYNX-CAM (all p = 0.014) because the view was obstructed by the camera holder and borescope camera. With both direct and camera-assisted views, visualization scores were higher at inter-incisor gaps ≥ 4 cm compared with 2 cm (all p < 0.05). CONCLUSIONS AND CLINICAL RELEVANCE: During laryngoscopy and intubation, the VLVET and borescope facilitated both direct and video laryngoscopy at distanceLARYNX-CAM in Beagle dogs when inter-incisor gaps were ≥ 4 cm.

Perianesthetic metabolic acidosis is associated with 2% dorzolamide eye drops in dogs that underwent ophthalmic surgery: a retrospective study (2019-2022).

OBJECTIVE: To evaluate whether the administration of 2% dorzolamide ophthalmic solution in dogs undergoing ophthalmic surgery is associated with perianesthetic metabolic acidosis. ANIMALS: 60 dogs, with or without dorzolamide administration, underwent arterial blood gas analysis immediately after anesthesia for ophthalmic surgery between 2019 and 2022; a total of 60 surgeries were evaluated. METHODS: This was a retrospective cross-sectional study. Logistic regression analysis was performed to investigate the association between the administration of 2% dorzolamide ophthalmic solution in dogs and the development of metabolic acidosis. Additionally, the influence of various potential risk factors, including age, body weight, sex, use of topical or systemic NSAIDs, and preoperative medications on the occurrence of metabolic acidosis, was evaluated. RESULTS: A significant association was found between the use of 2% dorzolamide ophthalmic solution and perianesthetic metabolic acidosis (OR, 6.79; 95% CI, 2.00 to 23.02; P = .002). Furthermore, topical dorzolamide administration was significantly associated with both perianesthetic hypokalemia (OR, 3.52; 95% CI, 1.11 to 11.20; P = .033) and perianesthetic hyperchloremia (OR, 9.25; 95% CI, 1.71 to 50.01; P = .010). CLINICAL RELEVANCE: The use of 2% dorzolamide ophthalmic solution is associated with perianesthetic metabolic acidosis, hypokalemia, and hyperchloremia in dogs. It is prudent to be aware of these risks, especially before anesthesia.

Evaluation of an automatic approach device to the epidural space of Beagle dogs.

OBJECTIVE: To compare the epidural anesthesia device (EPIA), which facilitates an automatic approach to location of the epidural space, with the performance of clinicians using tactile sensation and differences in pressure when inserting an epidural needle into the epidural space of a dog. STUDY DESIGN: Prospective, crossover experiment. ANIMALS: A total of 14 Beagle dogs weighing 7.5 ± 2.4 kg (mean ± standard deviation). METHODS: Each dog was anesthetized three times at 2 week intervals for three anesthesiologists (two experienced, one novice) to perform 14 epidural injections (seven manual and EPIA device each). The sequence of methods was assigned randomly for each anesthesiologist. The dogs were anesthetized with medetomidine (10 µg kg-1), alfaxalone (2 mg kg-1) and isoflurane and positioned in sternal recumbency with the pelvic limbs extended cranially. Epidural puncture in the manual method was determined by pop sensation, hanging drop technique and reduced injection pressure, whereas using the device a sudden decrease in reaction force on the device was detected. A C-arm identified needle placement in the epidural space, and after administration of iohexol (0.3 mL), the needle length in the epidural space was defined as the mean value measured by three radiologists. Normality was tested using the Kolmogorov-Smirnov test, and significant differences between the two methods were analyzed using an independent sample t test. RESULTS: In both methods, the success rates of epidural insertion were the same at 95.2%. The length of the needle in the epidural space using the device and manual methods was 1.59 ± 0.50 and 1.68 ± 0.88 mm, respectively, with no significant difference (p = 0.718). CONCLUSIONS AND CLINICAL RELEVANCE: EPIA device was comparable to human tactile sense for an epidural needle insertion in Beagle dogs. Further research should be conducted for application of the device in clinical environments.

Agreement Between Tongue-Based Oscillometric and Invasive Blood Pressure in Anesthetized Dogs of Various Weights.

This study aimed to evaluate the agreement between oscillometric blood pressure (OBP) measured from the tongue and invasive blood pressure (IBP) measured from the dorsal pedal artery in anesthetized dogs of various body weights. Forty-five client-owned dogs undergoing general anesthesia for surgery or imaging scan were included; weights ranged from 2.5 to 42.6 kg. Agreement between paired IBP and OBP during normotension was verified with reference standards used in small animals and humans. The data were stratified by body weight (≤5 kg versus >5 kg). In the >5 kg group (n = 29), the bias ± standard deviation for mean (2.1 ± 7.9 mm Hg) and diastolic pressure (-2.7 ± 7.9 mm Hg) exhibited reliability that met human standards (<5 ± 8 mm Hg). However, in the ≤5 kg group (n = 16), the bias ± standard deviation met only veterinary standards (≤10 ± 15 mm Hg) for mean (3.1 ± 10.2 mm Hg) and diastolic pressure (-2.5 ± 12.6 mm Hg). Agreement for systolic pressure did not meet either standard for both groups. This study demonstrates that tongue-based OBP is a close estimate of mean/diastolic blood pressure in anesthetized dogs (>5 kg) during normotension by small-animal and human criteria.

Transillumination facilitates coccygeal arterial cannulation in anaesthetised small-breed dogs.

BACKGROUND: Arterial access in small-breed dogs is challenging, but arterial visualisation may facilitate the procedure, as evidenced in human medicine. This prospective, randomised study investigated the result of using transillumination to guide coccygeal artery cannulation in small-breed dogs. METHODS: Coccygeal artery cannulation was attempted in dogs anaesthetised with butorphanol, midazolam, propofol and sevoflurane. In 70 dogs (standard technique group [STECHNIQUE group]), arterial cannulation was performed using pulse palpation. In 71 dogs (transilluminating-standard technique group [TSTECHNIQUE group]), a transilluminating device was utilised. The device was placed on the dorsal tail, and the cannula was advanced towards the dark line visible against the bright background. If the artery was not well visualised due to pigmentation, pulse palpation was used. The success rate of arterial cannulation was compared between the groups using the chi-squared test. RESULTS: Arterial cannulation was significantly more successful (p < 0.001) in the TSTECHNIQUE group (63/71 [88.7%]) than in the STECHNIQUE group (43/70 [61.4%]). LIMITATIONS: The number of punctures attempted and the time to cannulation were not assessed, and the procedure was only performed by veterinarians experienced in arterial cannulation. CONCLUSIONS: The transillumination allows for a more accurate approach to the coccygeal artery, improving the success of arterial cannulation when combined with pulse palpation.

Effect of temperature-responsive hydrogel on femoral and sciatic nerve blocks using bupivacaine in Beagle dogs.

OBJECTIVES: To compare the duration of regional anesthesia of the pelvic limb using bupivacaine with and without a temperature-responsive hydrogel (TRH) in dogs. METHODS: Under anesthesia using medetomidine (10 µg·kg-1 ), alfaxalone (2 mg·kg-1 ), and isoflurane, seven healthy male Beagles received four injections of 0.5% bupivacaine (1 mg·kg-1 with 5 µg·ml-1 epinephrine) to block the femoral and sciatic nerves bilaterally via ultrasound with nerve stimulation guidance. Bupivacaine was used on one pelvic limb (Bup treatment), and bupivacaine with TRH was used on the contralateral limb (Bup-TRH treatment). The nerve block was considered successful upon the absence of responses to pinching the digital pads and mid-tibial skin of both pelvic limbs with mosquito forceps; the pinch, proprioception, and locomotion tests were performed before (baseline) and at each hour after the nerve block until sensory and motor functions returned to baseline. The effect of TRH on nerve blocks was analyzed using a linear mixed model. RESULTS: The duration of the sensory nerve block at the digital pads and mid-tibial skin was longer with Bup-TRH (8.0 ± 1.6 h and 10.9 ± 1.6 h, respectively) than with Bup treatment (3.7 ± 2.0 h and 8.0 ± 1.6 h, respectively). Motor block times of proprioception and locomotion were longer with Bup-TRH (9.3 ± 1.6 and 12.7 ± 1.5 h, respectively) than with Bup treatment (4.6 ± 1.9 and 9.6 ± 1.5 h, respectively). No complications were observed. CLINICAL SIGNIFICANCE: TRH extended the duration of regional anesthesia of the pelvic limb using bupivacaine.

Cardiovascular Responses to Nasal Stimulation Under Ethmoidal-Maxillary Nerve Block and Lidocaine Gel in Anesthetized Beagles.

This study aimed to compare the effect of ethmoidal-maxillary nerve block (EMBLOCK) and topical application of 2% lidocaine gel (LGEL) on cardiovascular variables (heart rate and arterial blood pressure) during mechanical stimulation of the nasal cavity. The working hypothesis was that both techniques (EMBLOCK and LGEL) similarly blunt the cardiovascular responses to the stimulation. Six beagles were anesthetized using alfaxalone and isoflurane on three occasions with each treatment (EMBLOCK, LGEL, and control) in random order. The nasal cavity from the nose tip to the medial canthus was stimulated with a standard-tip cotton swab, and cardiovascular variables were recorded before and after stimulation. The maximum increases in heart rate and blood pressure were significantly smaller in EMBLOCK and LGEL than in the control. There was no significant difference between EMBLOCK and LGEL. Therefore, although further clinical studies are required, EMBLOCK and LGEL can be used because both EMBLOCK and LGEL attenuated the cardiovascular response to mechanical stimulation of the nasal cavity in dogs.

Effect of the respiratory rate on the pulse pressure variation induced by hemorrhage in anesthetized dogs.

BACKGROUND: Studies on anesthetized dogs regarding pulse pressure variation (PPV) are increasing. The influence of respiratory rate (RR) on PPV, in mechanically ventilated dogs, has not been clearly identified. OBJECTIVES: This study evaluated the influence of RR on PPV in mechanically ventilated healthy dogs after hemorrhage. METHODS: Five healthy adult Beagle dogs were premedicated with intravenous (IV) acepromazine (0.01 mg/kg). Anesthesia was induced with alfaxalone (3 mg/kg IV) and maintained with isoflurane in 100% oxygen. The right dorsal pedal artery was cannulated with a 22-gauge catheter for blood removal, and the left dorsal pedal artery was cannulated and connected to a transducer system for arterial blood pressure monitoring. The PPV was automatically calculated using a multi-parameter monitor and recorded. Hemorrhage was induced by withdrawing 30% of blood (24 mL/kg) over 30 min. Mechanical ventilation was provided with a tidal volume of 10 mL/kg and a 1:2 inspiration-to-expiration ratio at an initial RR of 15 breaths/min (baseline). Thereafter, RR was changed to 20, 30, and 40 breaths/min according to the casting lots, and the PPV was recorded at each RR. After data collection, the blood was transfused at a rate of 10 mL/kg/h, and the PPV was recorded at the baseline ventilator setting. RESULTS: The data of PPV were analyzed using the Friedman test followed by the Wilcoxon signed-rank test (p < 0.05). Hemorrhage significantly increased PPV from 11% to 25% at 15 breaths/min. An increase in RR significantly decreased PPV from 25 (baseline) to 17%, 10%, and 10% at 20, 30, and 40 breaths/min, respectively (all p < 0.05). CONCLUSIONS: The PPV is a dynamic parameter that can predict a dog's hemorrhagic condition, but PPV can be decreased in dogs under high RR. Therefore, careful interpretation may be required when using the PPV parameter particularly in the dogs with hyperventilation.

Measurement of tracheal diameters using ultrasonography versus computed tomography in Beagle dogs.

OBJECTIVE: To compare ultrasonography with computed tomography (CT) for assessment of tracheal diameter as a feasibility study for endotracheal tube selection. STUDY DESIGN: Prospective study. ANIMALS: A total of nine Beagle dogs with a median (interquartile range) weight of 7.4 (7.2-7.7) kg. METHODS: Tracheal diameter measurements were obtained at two locations: 1 cm proximal to caudal border of the cricoid cartilage (sublaryngeal; SL) and dorsal to above cranial border of the manubrium (thoracic inlet; TI). For CT, dogs were anesthetized with propofol and sevoflurane, in sternal recumbency, and measurements obtained after controlled ventilation-induced apnea and the endotracheal tube cuff was deflated. Transverse diameter, right and left 45° oblique diameters were measured. For ultrasonography, unsedated dogs were standing with slight neck extension, and images obtained in ventrodorsal, 45° right and left oblique ways after expiration. Diameters between the tracheal lumen mucosal borders were measured. The degree of agreement between the tracheal diameters measured at SL and TI locations with CT (TDCT-SL and TDCT-TI) and ultrasonography (TDUS-SL and TDUS-TI) was verified using the Bland-Altman method. RESULTS: The agreement between the measurements obtained with CT and ultrasonography was revealed by Bland-Altman analyses, although ultrasonography tended to slightly underestimate the tracheal diameter. CONCLUSIONS AND CLINICAL RELEVANCE: Ultrasonography can be applied for tracheal diameter measurement. Although further studies are required, an endotracheal tube selection method, using ultrasonography, could be proposed.

Modified retrograde intubation through the cricothyroid membrane in a cat with temporomandibular joint ankylosis.

A 6-month-old, 2.9-kg, male, Korean short hair cat was referred for inadequate mouth opening (4 mm), and condylectomy was scheduled for ankylosis of the left temporomandibular joint. Retrograde intubation via the cricothyroid membrane was planned since direct visualisation of the larynx was not possible. In dorsal recumbency, the cricothyroid membrane was punctured with an 18-gauge needle, and a J-tip guidewire, inserted via the needle, was advanced and identified at the mouth. A 6-Fr truncated feeding tube was inserted from the mouth to the cricothyroid membrane along the guidewire. The guidewire was removed, and the tip of the feeding tube was advanced as an anterograde guide to the inner trachea. Subsequently, an endotracheal tube was inserted along the feeding tube. Capnography was used to confirm correct intubation. Condylectomy was performed on the left temporomandibular joint, and the patient recovered from anaesthesia uneventfully. Retrograde intubation is less invasive than tracheostomy or cricothyroidotomy, and the cricothyroid membrane has been suggested as a suitable site for guidewire insertion in humans and dogs. However, when resistance is encountered during the advancement of endotracheal tube using the traditional technique without the anterograde guide, it may be difficult to distinguish where the resistance occurred leading to damage or oesophageal displacement in cats with relatively fragile airway. This report suggests that retrograde intubation via the cricothyroid membrane can be performed in cats with limited visualisation of the larynx, and an anterograde guide following the retrograde wire could reduce the potential damage or oesophageal displacement.

Effect of extension tube length on the damping coefficient and natural frequency in normotensive dogs.

OBJECTIVE: To establish an acceptable extension tube length (ETL) to measure direct blood pressure (BP) in dogs. DESIGN: Prospective, experimental study. SETTING: University-based small animal research facility. ANIMALS: Eight healthy Beagle dogs: 6 males and 2 females. INTERVENTIONS: Each extension tube with lengths of 25, 50, 75, 115, 145, 205, and 275 cm were connected after the catheterization with a 22-Ga catheter in the dorsal pedal artery in sternal recumbency. A square wave from the fast-flush test was consecutively recorded 5 times to analyze the system's dynamic response characteristics according to the ETL. After recording the square wave, the ETL was converted to a Latin square. The dynamic response was analyzed using natural frequency (NF) and the damping coefficient (DC), both of which affect the damping factor. The average values of NF and DC were plotted against a graph showing the damping factor. Linear regression was used to evaluate the between-group changes in NF and DC. MEASUREMENTS AND MAIN RESULTS: The DC gradually increased from 0.21 to 0.29 ξ, and the NF gradually decreased from 38 to 14 Hz according to the increase in ETL (P < 0.05). The dynamic response showed adequate damping with all ETLs. CONCLUSION: With an increase in ETL, the NF decreased significantly, while the DC demonstrated a less significant change. Therefore, NF had a greater influence on the damping factor of arterial BP measurement. There was no difference between ETLs from 25 to 275 cm lines for measuring BP. An ETL of less than 275 cm is recommended as the damping amount is adequate. Moreover, an ETL less than 275 cm does not meaningfully affect BP measurement in dogs.

Sealing peri-cuff leak of a small endotracheal tube passing through laryngeal web in five dogs (2015-2019).

CASE DESCRIPTION: 5 dogs with a history of ventriculocordectomy were anesthetized with isoflurane for ovariohysterectomy, dental prophylaxis, or intracapsular lens extraction. CLINICAL FINDINGS: No remarkable, abnormal clinical signs such as exercise intolerance, respiratory distress, or stridor were found in 4 dogs. However, 1 dog had cough after drinking, which had started after the ventriculocordectomy. During intubation, laryngeal web, suspected to be a complication of ventriculocordectomy, was accidentally discovered. TREATMENT AND OUTCOME: Laryngeal web was observed during intubation, necessitating the use of a smaller-diameter endotracheal tube (ETT) for intubation. However, the smaller cuff volume of the smaller ETT did not prevent the air leak. Therefore, the ETT with the inflated cuff was pulled cranially until the narrowed laryngeal lumen was plugged with the cuff behind the vocal cords. The ETT was secured to prevent slippage. No air leakage around the ETT cuff or complications related to the ETT placement were observed in the peri-anesthetic period in any dog. CLINICAL RELEVANCE: Laryngeal web can be found in patients with a history of larynx-related surgeries and may allow only a small-diameter ETT to pass through. The sealing technique used for peri-cuff air leak using a small ETT described here that can pass through a laryngeal web could be useful to seal an air leak around the cuff without complications.

Evaluation of the tongue for oscillometric measurement of arterial pressure in anesthetized Beagle dogs.

OBJECTIVE: To evaluate the agreement between oscillometric blood pressure (OBP) measured from the tongue and invasive blood pressure (IBP), and to compare OBPs measured from the tongue with OBPs measured from the pelvic limb and tail. STUDY DESIGN: Prospective experimental study. ANIMALS: A total of eight adult Beagle dogs weighing 11.1 ± 1.2 kg. METHODS: Animals were premedicated with intravenous (IV) acepromazine (0.005 mg kg-1). Anesthesia was induced with alfaxalone (3 mg kg-1) IV and maintained with isoflurane. The dorsal pedal artery was catheterized for IBP measurements. Systolic (SAP), diastolic (DAP) and mean (MAP) arterial pressure were simultaneously measured from the tongue, pelvic limb and tail. Based on invasive SAP, hypertension (>140 mmHg), normotension (90-140 mmHg) and hypotension (<90 mmHg) were induced by controlling end-tidal isoflurane concentrations and/or dobutamine/dopamine administration. Agreement between paired IBP and OBP measurements was analyzed with reference standards for noninvasive blood pressure devices used in small animals and humans. RESULTS: Regardless of cuff placement, the mean bias ± standard deviation between IBP and OBP met veterinary (≤10 ± 15 mmHg) and human (<5 ± 8 mmHg) standards for MAP and DAP. SAP measurements provided by the OBP device showed unacceptable agreement with IBP, and the bias between methods increased at higher blood pressures, regardless of cuff site. During hypotension, tongue OBP showed the largest percentage of absolute difference <10 mmHg in relation to IBP for SAP (90%), MAP (97%), and DAP (93%), compared with pelvic limb (60%, 97% and 82%, respectively) and tail OBP (54%, 92% and 77%, respectively). CONCLUSIONS AND CLINICAL RELEVANCE: The tongue is a clinically useful site for measuring OBP in anesthetized Beagle dogs, providing reliable estimates of MAP and DAP. The tongue could replace other cuff placement sites and may be a relatively suitable site for assessing hypotension.

Effect of intra-abdominal hypertension on the intraocular pressure of the conscious dogs.

This study was performed to evaluate the effect of intra-abdominal pressure (IAP) on intraocular pressure (IOP) in conscious dog models using a balloon technique to generate intra-abdominal hypertension. Six healthy dogs without ocular abnormalities were evaluated in this study. A balloon device was placed in the intra-abdominal cavity. The abdomen was insufflated to IAP levels of 15 and 25 mmHg using the balloon device. Intraocular pressure was measured at baseline, at IAP levels of 15 and 25 mmHg, and after decompression. In comparison with the mean baseline IOP (15.1 ± 2.0 mmHg), there was a significant increase in IOP at IAP levels of 15 mmHg (20.0 ± 2.1 mmHg) and 25 mmHg (19.9 ± 2.2 mmHg), corresponding to a 32.4% and 31.7% increase from baseline IOP, respectively. The mean IOP after decompression (14.8 ± 1.7 mmHg) was significantly lower compared to those at IAP levels of 15 and 25 mmHg. The present findings demonstrate that increased IAP has a clinically significant effect on IOP in dogs under conscious conditions. Although more research is needed to determine of increased IAP on IOP, these findings suggest that increased IAP leads to mild and reversible increase in IOP.

The effects of maxillary nerve block, ethmoidal nerve block and their combination on cardiopulmonary responses to nasal stimulation in anesthetized Beagle dogs.

OBJECTIVE: To describe an approach for ethmoidal nerve block (EBLOCK) and to compare the effects of a maxillary nerve block (MBLOCK), EBLOCK and their combination (M-EBLOCK) on heart rate (HR), systolic (SAP), mean (MAP), diastolic (DAP) arterial pressures and respiratory rate (fR) during nasal stimulation in dogs. STUDY DESIGN: Prospective, blinded, randomized, crossover placebo-controlled study. ANIMALS: Beagle dogs (five cadavers, nine live dogs), with a median (interquartile range) weight of 10.5 (10.3-11.0) kg. METHODS: The accuracy of iohexol injections (each 1 mL) at the maxillary and ethmoidal foramina in cadavers was evaluated using computed tomography. Then, anesthetized dogs were administered four bilateral treatments separated by 1 week, saline or 2% lidocaine 1 mL per injection: injections of saline at the maxillary and ethmoidal foramina (Control), injections of lidocaine at the maxillary foramina and saline at the ethmoidal foramina (MBLOCK), injections of saline at the maxillary foramina and lidocaine at the ethmoidal foramina (EBLOCK) and injections of lidocaine at all foramina (M-EBLOCK). The ventral nasal meatus was bilaterally stimulated using cotton swabs, and HR, SAP, MAP, DAP and fR were continuously recorded. Values for each variable were compared before and after stimulation using Wilcoxon signed-rank test. Changes in variables among treatments were analyzed using Mann-Whitney U and Kruskal-Wallis tests (p ≤ 0.05). RESULTS: Computed tomography revealed iohexol distribution around the openings of the target foramina in all cadavers. In living dogs, HR, SAP, MAP, DAP and fR significantly increased after stimulation within each treatment (p < 0.03). Physiologic responses were significantly attenuated, but not absent, in the M-EBLOCK [HR (p = 0.019), SAP, MAP, DAP and fR (all p ≤ 0.001)] compared with those in the Control. CONCLUSIONS AND CLINICAL RELEVANCE: Concurrent injections of lidocaine at the maxillary and ethmoidal foramina attenuated HR, arterial pressure and fR responses to nasal stimulation in Beagle dogs.

Fluoroscopy-guided approach to the lumbar dorsal root ganglion in dogs: cadaver study in Beagles.

OBJECTIVE: To evaluate an approach to the canine lumbar dorsal root ganglion (DRG), a significant contributor to the pain pathway, using new methylene blue staining. STUDY DESIGN: Prospective randomized study. ANIMALS: A total of three Beagle dog cadavers weighing 10.4 ± 0.7 kg (mean ± standard deviation). METHODS: Bilateral third to fifth lumbar DRG approaches were performed in three dog cadavers positioned in sternal recumbency. The mammillary process was palpated, and a 22 gauge spinal needle was inserted through the skin 1 cm lateral to the process and directed towards the median plane at a 45° angle to the dorsal plane. The needle was advanced along the transverse plane until touching bone, or a popping sensation was detected. Under fluoroscopic guidance, the position of the needle tip was adjusted to be in the cranioventral part of the intervertebral foramen. The location of the needle was confirmed by demarcation of the nerve roots after iohexol (0.1 mL) injection. For evaluation of the DRG approach, new methylene blue (0.1 mL) was injected. Subsequently, anatomical dissection of the area was performed. The DRG staining was scored as follows: 0, no staining; 1, partial (<50%); 2, partial (≥50%); and 3, complete staining. Comparisons among the staining scores of the third to fifth DRG were assessed with the Friedman test. RESULTS: Staining score 3 was achieved in 14 of 18 (77.8%) sites. Staining scores 2, 1 and 0 were identified at two, one and one of the 18 sites, respectively. No significant difference was noted in the staining scores among the third to fifth DRGs (p = 0.78). CONCLUSIONS AND CLINICAL RELEVANCE: The technique used for DRG injections achieved adequate DRG staining, supporting use of the fluoroscopy-guided approach to the canine lumbar DRG.

Effect of intra-abdominal hypertension on plasma exogenous creatinine clearance in conscious and anesthetized dogs.

OBJECTIVE: To evaluate the effect of intra-abdominal pressure (IAP) on plasma exogenous creatinine clearance in both conscious and anesthetized dog models using a balloon technique to generate intra-abdominal hypertension. DESIGN: Prospective, cross-over, experimental study. SETTING: University-based small animal research facility. ANIMALS: Six healthy male Beagle dogs. INTERVENTIONS: A balloon device comprising a Foley urinary catheter and latex balloon was placed in the intra-abdominal cavity. Plasma exogenous creatinine clearance was compared after intravenous administration of exogenous creatinine solution at 80 mg/kg under 4 different treatment conditions as follows: control and IAP levels of 25 mm Hg in conscious dogs and control and IAP levels of 25 mm Hg in anesthetized dogs (CC, C25, AC, and A25, respectively). Samples were obtained before (T0) and 10, 20, 30, 60, 90, 120, 240, 360, 480, and 600 min after administration of creatinine in all treatment groups. MEASUREMENTS AND MAIN RESULTS: There were no significant differences in plasma creatinine concentration for CC, AC, and C25 during the treatment period. However, in the A25 treatment condition, the plasma creatinine concentration increased significantly at 10, 20, 30, 60, 90, and 120 min after administration of creatinine (P < 0.05). Plasma creatinine clearances were 5.0 ± 0.5, 4.7 ± 1.2, 5.5 ± 0.9, and 2.5 ± 0.5 mL/kg/min for 600 min (CC, AC, C25, and A25, respectively). In the A25 treatment condition, the plasma exogenous creatinine clearance decreased significantly to 50%, 47%, and 55% of that under control conditions (CC, AC, and C25, respectively). After decompression of the abdomen, plasma creatinine concentrations declined rapidly and returned to basal concentrations. CONCLUSIONS: Intra-abdominal hypertension under general anesthesia could cause renal hypoperfusion. Timely decompression may improve the outcome of acutely increased IAP when surgery and/or general anesthesia is required in canine patients.

Cranial versus caudal thoracic epidural anesthesia using three volumes of lidocaine in conscious Beagle dogs.

OBJECTIVE: To compare the effects of epidural injection of three volumes of lidocaine injected at the third (T3) or eleventh thoracic vertebra (T11) in conscious dogs to induce thoracic epidural anesthesia (TEA) and to measure the epidural dispersion of iohexol under similar conditions. STUDY DESIGN: Prospective crossover experiment. ANIMALS: A group of five Beagle dogs weighing 10.4 ± 0.5 kg (mean ± standard deviation). METHODS: Each dog was anesthetized twice, separated by 1 week, for inserting an epidural catheter at the lumbosacral space and advancing the tip to T3 (treatment TEAT3) or T11 (treatment TEAT11). For each treatment, three volumes of 2% lidocaine (0.05, 0.10 and 0.20 mL kg-1) were administered at 24 hour intervals, and sensory blockade (SB) of dermatomes was estimated by pinching the skin with mosquito forceps. Under identical conditions of injection volume and site, iohexol was administered 3 hours after lidocaine injection to identify epidural distribution (ED) using computed tomography. The effects of injection site and volume on SB of thoracic dermatomes and ED were analyzed using a linear mixed model (p < 0.05). RESULTS: Thoracic SB and ED significantly increased as the volume increased (p < 0.001 and p < 0.001, respectively), and significantly decreased in TEAT3 than in TEAT11 (p = 0.011 and p = 0.002, respectively). Cervical SB was obtained in three of five dogs in TEAT3 and two of five dogs in TEAT11 injected with 0.20 mL kg-1. One dog showed temporary inspiratory stridor probably caused by bilateral laryngeal paralysis, but no hypoxia. CONCLUSIONS AND CLINICAL RELEVANCE: TEA induced at T3 produced less thoracic SB than did TEA at T11 with the same volumes of lidocaine. The cervical SB obtained with the highest volume of lidocaine may increase the risk of laryngeal paralysis and pulmonary aspiration.