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J Virol Methods ; 168(1-2): 78-81, 2010 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-20435067

RESUMO

New Zealand identified its first pandemic H1N1 influenza cases in late April 2009, immediately prior to the historical start of the New Zealand influenza season. Both pandemic and oseltamivir-resistant seasonal H1N1 viruses cocirculated in the population for a period of time. Thus, concerns were raised about the possibility of reassortment events between the two strains. An RT-PCR-based genotyping assay was developed so that H1N1 influenza coinfections and reassortants could be detected quickly. The assay differentiated effectively the seasonal and pandemic strains. It also confirmed the identification of the first reported coinfection of pandemic and seasonal H1N1 strains during the 2009 Southern Hemisphere influenza season in New Zealand.


Assuntos
Impressões Digitais de DNA/métodos , Vírus da Influenza A Subtipo H1N1/classificação , Vírus da Influenza A Subtipo H1N1/genética , Vírus Reordenados/classificação , Vírus Reordenados/genética , Virologia/métodos , Humanos , Vírus da Influenza A Subtipo H1N1/isolamento & purificação , Influenza Humana/virologia , Nova Zelândia , RNA Viral/genética , Vírus Reordenados/isolamento & purificação , Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos
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