Bioprospecting of exopolysaccharide from marine Sphingobium yanoikuyae BBL01: Production, characterization, and metal chelation activity.

In the present study, an exopolysaccharide (EPS)-producing bacterial strain was isolated from the Eastern Sea (Sokcho Beach) of South Korea and identified as Sphingobium yanoikuyae BBL01. Media optimization was performed using response surface design, and a yield of 2.63 ± 0.02 g/L EPS was achieved. Purified EPS produced using lactose as the main carbon source was analyzed by GC-MS and found to be composed of α-D-xylopyranose (28.6 ± 2.0%), ß-D-glucopyranose (21.0 ± 1.6%), α-D-mannopyranose (18.5 ± 1.2%), ß-d-mannopyranose (13.1 ± 1.4%), ß-D-xylopyranose (10.2 ± 2.1%), α-d-talopyranose (5.9 ± 1.1%), and ß-d-galacturonic acid (2.43 ± 0.8%). Interestingly, different carbon sources (glucose, galactose, glycerol, lactose, sucrose, and xylose) showed no effect on EPS monomer composition, with a slight change in the mass percentage of various monosaccharides. Purified EPS was stable up to 233 °C, indicating its possible suitability as a thickening and gelling agent for food-related applications. EPS also showed considerable emulsifying, flocculating, free-radical scavenging, and metal-complexion activity, suggesting various biotechnological applications.

Microfluidic fabrication of photo-responsive hydrogel capsules.

We report thermo- and photo-responsive hydrogel capsules, providing controlled encapsulation and triggered release of water-soluble encapsulants. Monodisperse O/W/O (oil-in-water-in-oil) double-emulsion drops are produced in a capillary microfluidic device as templates, which transform into hydrogel capsules upon polymerization of thermo-sensitive monomers in the water phase containing gold nanorods.