Assessment of the presence of UV filters and UV stabilizers in stranded dolphin blubber.

The presence of ultraviolet filters (UVFs) and stabilizers (UVSs) was evaluated for the first time in the common bottlenose dolphin (Tursiops truncatus). UVFs and UVSs are compounds of growing concern because their effects on the environment are not completely known. UVFs and UVSs are added to personal care products (PCPs), such as cosmetics and products related to sun care and once released to the aquatic ecosystem, marine organisms can bioaccumulate these substances. This work aimed to determine the presence of 12 UVFs and UVSs in cetacean blubber samples to assess the pollution to which these animals of the highest trophic chain levels are exposed due to human activity. Analytical determinations were carried out using a method based on microwave-assisted extraction combined with ultrahigh-performance liquid chromatography and tandem mass spectrometry detection. The developed method was successfully applied to determine the target compounds in the blubber tissues of five necropsied common bottlenose dolphins. Three of the 12 studied compounds, namely 2-ethylhexyl 2-cyano-3,3-diphenylprop-2-enoate (octocrilene, OC), 2-hydroxy-4-methoxybenzophenone (benzophenone 3, BP3) and 3-methylbutyl (E)-3-(4methoxyphenyl) prop-2-enoate (IMC), were detected in several samples. Of the identified compounds, OC was present in all the samples and at the highest concentration within the range from 52.61 ± 18.59 to 108.0 ± 11.32 ng·g-1.

Seafood consumption as a source of exposure to high production volume chemicals: A comparison between Catalonia and the Canary Islands.

Seafood plays an important role in diet because of its health benefits. However, the fact that chemical compounds such as high production volume chemicals may be present in seafood means that its consumption can be a potential risk for population. To assess the occurrence of HPVs and estimate the exposure and risk associated with their consumption, specimens of the most consumed seafood species in Catalonia and the Canary Islands, Spain, were collected and analysed. Results showed higher levels of HPVs in samples from Catalonia and a prevalence of phthalate esters and benzenesulfonamides over the other target compounds in samples from both locations. Multivariate analysis showed spatial differences between the mean concentration profiles of HPVs for the samples from Catalonia and the Canary Islands. Exposures were higher for the samples from Catalonia, although the intake of HPVs via seafood was not of any real concern in either of the locations.

Assessment of anthropogenic pollution by UV filters using macrophytes as bioindicators.

Marine environment pollution has increased in recent decades as a result of anthropogenic activities. Macrophytes can assimilate the compounds dissolved in the water and respond to changes in surround conditions, for that, they can be used as bioindicators of pollution in aquatic environments. Currently organic ultraviolet (UV) filters have shown ever-increasing in pollution levels in marine ecosystems. The anthropogenic pollution produced by eight organic ultraviolet (UV) filters in coastal macrophytes was studied. A microwave-assisted extraction (MAE), followed by ultrahigh-performance liquid chromatography with tandem mass spectrometry (UHPLC-MS/MS) was applied to 76 macrophyte (seaweeds and seagrass) samples from three different beaches on the Gran Canaria Island (Spain), collected for 6 months. All studied UV filters were found with different detection frequencies from 16% to 100% in macrophyte samples. Octocrylene (OC) was detected in all the analysed samples throughout the sampling period. The highest concentration, 19,369 ng·g-1 dry weight (dw), was for this compound in the seagrass Cymodocea nodosa. The bioconcentration ratio was determined for several seaweed groups (red, brown, green). Different bioconcentration grades were obtained. Those above 1000 indicated significant accumulation, which increases the possibility of chronic effects on seaweed and at upper tropic levels.

Occurrence and environmental hazard of organic UV filters in seawater and wastewater from Gran Canaria Island (Canary Islands, Spain).

Organic ultraviolet (UV) filters are used in personal care products, but they are also added to industrial products and are constantly released to the environment. This study analyses the occurrence of 8 widely used organic UV filters in seawater from three beaches on the Gran Canaria Island (Spain) and in three wastewater treatment plants (WWTPs) by taking samples from influents and effluents. It also discusses the target compounds' post-treatment removal efficiencies. Sampling was carried out for 6 months and analytes were extracted by solid phase extraction with Sep-pak C18 cartridges. They were determined by ultra-high performance liquid chromatography coupled to mass spectrometry in tandem. The potential environmental hazard associated with the found concentrations was also assessed for marine organisms. Different target compounds were detected on the analysed beaches and in the wastewater. Benzophenone-3 (BP3) was the most recurrent compound in the seawater samples (frequency detection of 83%) and also in wastewater influents and effluents (measured in all the samples). However, the highest concentrations for seawater (172 µg L-1) and influent wastewater (208 µg L-1) corresponded to octocrylene, while methylene bis-benzotriazolyltetramethylbutylphenol was the compound most concentrated in secondary treatment effluent (34.0 µg L-1) and BP3 in tertiary treatment effluent (8.07 µg L-1). All the analysed samples showed that at least one target UV filter was present. Regarding the removal efficiencies of these compounds in the studied WWTPs, consistent differences between the target compounds were observed in influent concentration terms, where the average removal rates were higher than 50% for most of the compounds. Conventional treatment is unable to completely remove many studied compounds, while tertiary treatment acts as an additional elimination for some of them. An environmental hazard quotient above 1 was found for octocrylene, benzophenone-3 and 4-methylbenzylidene camphor, which indicates a potential high hazard for living species if these compounds are present.

UV filters and UV stabilisers adsorbed in microplastic debris from beach sand.

Microplastics (MPs) in oceans adsorb different types of pollutants, which can negatively impact the food chain. The extensive use of personal care products (PCPs) has led to their ubiquitous environmental presence, and their partition between plastic matrices and surroundings is determined by their physico-chemical characteristics and environmental conditions. This work develops and applies a methodology to determine 12 UV filters (UVFs) and UV stabilisers (UVSs) in MPs collected in beach sand. The analyses were carried out by ultrasound-assisted extraction and ultrahigh-performance liquid chromatography with tandem mass spectrometry detection. The validated procedure was applied to MPs samples taken in sand samples from 13 beaches on the Canary Islands (Spain). The results showed the presence of 10 UV filters and UV stabilisers at concentrations between 1 and 4031 ng·g-1, where octocrylene was the most frequently found. The target analytes were present in all the sampling beaches.

Fabric Phase Sorptive Extraction of Selected Steroid Hormone Residues in Commercial Raw Milk Followed by Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry.

Hormones in edible matrices, such as milk, are a subject of concern because of their adverse effects on the endocrine system and cell signaling and the consequent disruption of homeostasis in human consumers. Therefore, the assessment of the presence of hormones in milk as potential endocrine-disrupting compounds is warranted. However, the complexity of milk as a sample matrix and the ultra-low concentration of hormones pose significant analytical challenges. Fabric phase sorptive extraction (FPSE) has emerged as a powerful analytical technique for the extraction of emerging pollutants from complex aqueous matrices. FPSE allows for substantially simplified sample handling and short extraction and desorption times, as well as the decreased use of organic solvents. It is considered a green alternative to traditional extraction methodologies. In this work, the FPSE technique was evaluated to perform the simultaneous extraction of 15 steroid hormones from raw milk without employing any sample pretreatment steps. Clean and preconcentrated hormone solutions obtained from FPSE of raw milk were analyzed using ultra-high-performance liquid chromatography-tandem mass spectrometry to achieve low detection limits, which ranged from 0.047 to 1.242 ng·mL-1. Because of the presence of many interferents in milk, such as proteins, lipids, and sugar, the effect of fat content on the extraction procedure was also thoroughly studied. Additionally, for the first time, the effect of lactose on the extraction of steroid hormones was evaluated, and the results showed that the extraction efficiencies were enhanced in lactose-free samples. Finally, the optimized methodology was applied to commercial samples of cow and goat milk, and no measurable concentrations of the studied hormones were detected in these samples.

Fate and distribution of benzotriazole UV filters and stabilizers in environmental compartments from Gran Canaria Island (Spain): A comparison study.

Tourism is an economic sector of great importance worldwide. In coastal areas, this activity is associated with the use of personal care products, such as ultraviolet (UV) filters and stabilizers. Therefore, assessing their presence and the exposure of living organisms to the impact of this kind of pollutant in such areas could be especially important. The Canary Islands (Spain) are considered an outermost region, and their main economic activity is based on tourism, both national and international. Thus, this area could be remarkably vulnerable to this kind of pollution, and its characterization could be useful to infer conclusions for other similar regions. With this aim, the occurrence of organic UV filters and stabilizers in different environmental matrices in Gran Canaria Island is presented in this work. Six benzotriazole compounds, UV-P, UV-326, UV-327, UV-328, UV-329 and UV-360, were found in wastewater, seawater, sludge, sediment, seaweed and fish samples. The numerous studies devoted to establishing the distribution of these target compounds in many different matrices on a touristic and particularly overcrowded island such as Gran Canaria can be used to understand the pollution situation in similar locations. The works in which determination procedures using different extraction techniques were optimized and validated for the analysis of liquid and solid samples are summarized. They are critically discussed regarding their characteristics and analytical parameters. This research is of interest to environmental managers specializing in the conservation of coastal areas where tourism is an important industry since the active components of UV filters and stabilizers can bioaccumulate and biomagnify in the trophic chain.

An assessment of the concentration of pharmaceuticals adsorbed on microplastics.

In the last decade, microplastics (MPs) have become an increasing cause for concerning. These particles are scattered throughout seas and oceans and have the capability of transporting adsorbed pollutants as pharmaceutical compounds, which can cause toxic effects and be transferred along the food chain. The development, optimization and validation of a sensitive and reliable analytical procedure for the extraction and determination of ten common pharmaceuticals adsorbed on MPs is reported in this study. This method involves ultrasound-assisted extraction coupled with ultra-high-performance liquid chromatography and tandem mass spectrometry. All of the variables included in the extraction process, such as the extraction time and type and solvent volume, were studied and optimised. Under optimal conditions, good reproducibility and repeatability, with relative standard deviations lower than 15% in most cases, were obtained while limits of detection between 0.25 and 15.8 ng g-1 were achieved. Last, the method was applied to the analysis of samples collected from beaches in the Canary Islands (Spain). The results indicated the presence of several analytes adsorbed on MPs in concentrations ranging from 34.0 to 111 ng g-1.

Multiresidue Analysis of Organic UV Filters and UV Stabilizers in Fish of Common Consumption.

Fish species can bioaccumulate different pollutants present in the marine environments and incorporate them into the trophic chain. In this work, the occurrence of organic ultraviolet (UV) stabilizers and filters in different species of fishes of high consumption has been studied. A multiresidue method based on microwave-assisted extraction and ultra-high performance liquid chromatography with mass spectrometry detection was developed and then it was applied to nine fish species from markets in the Canary Islands and Catalonia (Spain). Three UV filters (BP-3, OC and BM-DBM) and two stabilizers (UV-328 and UV-329) were found in some of the studied species, in concentrations ranging between 0.067 and 0.683 µg g-1 dry weight (dw). BP-3 (UV filter) was the most frequently detected compound, followed by UV-329 (UV stabilizer). Thunnus thynnus was the most heavily polluted species, with a concentration of 1.201 µg g-1 dw as the sum of all measured compounds.