[The concentration of procalcitonin in blood andi enteric exudation in patients at early post-operation period].

The study sampling included 46 patients operated on the occasion of acute surgical pathology of abdominal organs. The concentration of procalcitonin was analyzed at 2-3 days after operation in simultaneously obtained samples of blood serum and exudation of small intestine. The level of procalcitonin was analyzed using enzyme-linked immunosorbent assay with test-systems with sensitivity 0.01 ng/ml. The concentration of procalcitonin in content of small intestine was lower than in blood serum (p<0.001) and in most samples of enteric exudation (n=33) did not exceed 0.01 ng/ml. The analysis of correlation interdependences established that concentration of procalcitonin in intestinal exudation has no dependencies with its level in blood serum. The admixture of blood as a result of traumatization of mucous membrane under application of naso-jejunal probe is one of main causes of occurrence of procalcitonin in content of intestine.

[The impact of blood smear preparation on effectiveness of functioning of Vision Hema--the digital system of automatic blood analysis].

The article deals with study of the impact of standardization of blood smears preparation on effectiveness of functioning of Vision Hema system. The analysis was applied to the results of counting of 200 leukocytes in 30 blood smears prepared from venous blood stabilized with ethylenediaminetetraacetic acid using thoroughly degreased slide plates and applying automatic device to prepare blood smears (comparative group) and in 49 preparations prepared manually from non-stabilized capillary blood (control group). The standardization of the procedure of preparation of glass samples resulted in five time decrease of total amount of artifacts and in disappearance of thrombocytes aggregates and pavement epithelium cells. The absolute amount of destroyed leukocytes decreased in 2.4 times and particles of dirt in 9.5 time. The proposed technique of preparation of smear increased velocity of automatic analysis of leukogram by the Vision Hema system in 2 times and speeded up validation by physician of derived results in 3 times.

[Laboratory diagnosis of urinary tract injuries].

In 238 patients with various surgical diseases of the abdominal cavity, concentration of creatinine in serum and peritoneal cavity exudate was evaluated. In the most cases, no significant differences in creatinine levels were found in these biological fluids, that allowed to recommend to use creatinine level in the exudate, flowing along drainages, as a parameter for dynamic control of nitrogen-excretion renal function. Significant difference in the serum creatinine level and exudate creatinine level was found in cases of injury of organs of urinary system and the penetration of urine into the peritoneal cavity. In these cases (n = 16), creatinine level in the abdominal exudates exceeded the serum level (p < 0.01), that allowed to provide a new method for diagnosis of lesions of the urinary tract.

[Diagnostic value of studying creatinine in abdominal exudates].

The levels of creatinine in serum and peritoneal exudate were studied in 77 patients with acute surgical abdominal diseases. It has been found that the exudate concentration of this compound is mainly determined by its level in the serum, which allows the exudate creatinine to be studied for the diagnosis of renal failure. The creatinine concentration in the abdominal exudate in relation to that in the serum varies in urinary tract injury and in the early hours after abdominal sanitation in peritonitis. Exudate creatinine concentration increases (by 2.7-fold or more) require that the urinary tract should be revised to reveal its injury. Creatinine levels in the exudate can be determined not earlier than 8 hours after abdominal lavage. The determination of creatinine concentrations in the peritoneal exudate is a non-invasive and informative tool that enhances the quality of laboratory monitoring in emergency abdominal surgery.

[Lacrimal and salivary antioxidants in viral infection]. / Antioksidanty slezy i sliuny pri virusnoi infektsii.

The activities of antioxidant enzymes in the cornea and tears in patients with ophthalmic herpes and in the saliva in those with herpetic stomatitis were assessed. Impaired inhibition of hydroxy radical and a drop of antioxidant enzymes activities and of the level of ascorbic acid in herpes-infected cornea and tears are factors in the pathogenesis of ophthalmic herpes. Measurements of enzymatic activities in the tears and saliva in herpetic infection are a valuable diagnostic test and a criterion of treatment efficacy.

[The immunochemical determination of the concentration of organ-specific ejaculate proteins in the differential diagnosis of chronic inflammatory diseases of the male reproductive system]. / Immunokhimicheskoe opredelenie kontsentratsii organospetsificheskikh belkov éiakuliata v differentsial'noi diagnostike khronicheskikh vospalitel'nykh zabolevanii muzhskoi reproduktivnoi sistemy.

Concentrations of lactoferrin (LF), alpha-microglobulin of fertility (AMGF) and specific thermostable alpha-glycoprotein (STAG) which are markers of the prostate, seminal vesicles and testiculi in ejaculate, respectively, were determined by Ouchterlony's immunodiffusion method in 148 patients with chronic prostatitis (group 1), 26 patients with combination of prostatitis with chronic epididymitis (group 2) and 22 healthy controls (group 3). After that the patients were given prostatilen. It was found that high pretreatment values of LF registered in groups 1 and 2 lowered close to those in the controls. This shows elevated LF to be a diagnostic sign of prostatitis. In group 1 AMGF was lower than in controls, in groups 1, 2 and 3 STAG levels were similar.