Pharmacological Blockade of the Adenosine A2B Receptor Is Protective of Proteinuria in Diabetic Rats, through Affecting Focal Adhesion Kinase Activation and the Adhesion Dynamics of Podocytes.

Induction of the adenosine receptor A2B (A2BAR) expression in diabetic glomeruli correlates with an increased abundance of its endogenous ligand adenosine and the progression of kidney dysfunction. Remarkably, A2BAR antagonism protects from proteinuria in experimental diabetic nephropathy. We found that A2BAR antagonism preserves the arrangement of podocytes on the glomerular filtration barrier, reduces diabetes-induced focal adhesion kinase (FAK) activation, and attenuates podocyte foot processes effacement. In spreading assays using human podocytes in vitro, adenosine enhanced the rate of cell body expansion on laminin-coated glass and promoted peripheral pY397-FAK subcellular distribution, while selective A2BAR antagonism impeded these effects and attenuated the migratory capability of podocytes. Increased phosphorylation of the Myosin2A light chain accompanied the effects of adenosine. Furthermore, when the A2BAR was stimulated, the cells expanded more broadly and more staining of pS19 myosin was detected which co-localized with actin cables, suggesting increased contractility potential in cells planted onto a matrix with a stiffness similar to of the glomerular basement membrane. We conclude that A2BAR is involved in adhesion dynamics and contractile actin bundle formation, leading to podocyte foot processes effacement. The antagonism of this receptor may be an alternative to the intervention of glomerular barrier deterioration and proteinuria in the diabetic kidney disease.

Patients Age 40 Years and Younger With Multiple Myeloma Have the Same Prognosis as Older Patients: An Analysis of Real-World Patients' Evidence From Latin America.

PURPOSE: Multiple myeloma (MM) is a highly heterogeneous, incurable disease most frequently diagnosed in the elderly. Therefore, data on clinical characteristics and outcomes in the very young population are scarce. PATIENTS AND METHODS: We analyzed clinical characteristics, response to treatment, and survival in 103 patients with newly diagnosed MM age 40 years or younger compared with 256 patients age 41-50 years and 957 patients age 51 years or older. RESULTS: There were no statistical differences in sex, isotype, International Scoring System, renal involvement, hypercalcemia, anemia, dialysis, bony lesions, extramedullary disease, and lactate dehydrogenase (LDH). The most used regimen in young patients was cyclophosphamide, bortezomib, dexamethasone, followed by cyclophosphamide, thalidomide, dexamethasone and bortezomib, thalidomide, dexamethasone. Of the patients age 40 years or younger, only 53% received autologous stem-cell transplant (ASCT) and 71.1% received maintenance. There were no differences in overall survival (OS) in the three patient cohorts. In the multivariate analysis, only high LDH, high cytogenetic risk, and ASCT were statistically associated with survival. CONCLUSION: In conclusion, younger patients with MM in Latin America have similar clinical characteristics, responses, and OS compared with the elderly.

Targeting the Endothelin-1 pathway to reduce invasion and chemoresistance in gallbladder cancer cells.

BACKGROUND: Gallbladder cancer (GBC) is a prevalent and deadly biliary tract carcinoma, often diagnosed at advanced stages with limited treatment options. The 5-year survival rate varies widely from 4 to 60%, mainly due to differences in disease stage detection. With only a small fraction of patients having resectable tumors and a high incidence of metastasis, advanced GBC stages are characterized by significant chemoresistance. Identification of new therapeutic targets is crucial, and recent studies have shown that the Endothelin-1 (ET-1) signaling pathway, involving ETAR and/or ETBR receptors (ETRs), plays a crucial role in promoting tumor aggressiveness in various cancer models. Blocking one or both receptors has been reported to reduce invasiveness and chemoresistance in cancers like ovarian, prostate, and colon. Furthermore, transcriptomic studies have associated ET-1 levels with late stages of GBC; however, it remains unclear whether its signaling or its inhibition has implications for its aggressiveness. Although the role of ET-1 signaling in gallbladder physiology is minimally understood, its significance in other tumor models leads us to hypothesize its involvement in GBC malignancy. RESULTS: In this study, we investigated the expression of ET-1 pathway proteins in three GBC cell lines and a primary GBC culture. Our findings demonstrated that both ETAR and ETBR receptors are expressed in GBC cells and tumor samples. Moreover, we successfully down-regulated ET-1 signaling using a non-selective ETR antagonist, Macitentan, which resulted in reduced migratory and invasive capacities of GBC cells. Additionally, Macitentan treatment chemosensitized the cells to Gemcitabine, a commonly used therapy for GBC. CONCLUSION: For the first time, we reveal the role of the ET-1 pathway in GBC cells, providing insight into the potential therapeutic targeting of its receptors to mitigate invasion and chemoresistance in this cancer with limited treatment options. These findings pave the way for further exploration of Macitentan or other ETR antagonists as potential therapeutic strategies for GBC management. In summary, our study represents a groundbreaking contribution to the field by providing the first evidence of the ET 1 pathway's pivotal role in modulating the behavior and aggressiveness of GBC cells, shedding new light on potential therapeutic targets.

Multivariate optimization of methylene blue dye degradation using electro-Fenton process with self-doped TiO2 nanotube anode.

This paper reports the optimization of the electro-Fenton (EF) process using different anode materials for the degradation of Methylene Blue (MB) dye as a model compound. The cathode used was an air-diffusion PTFE, while three different anode materials (Pt, DSA, and self-doped TiO2 nanotubes - SD-TNT) were tested individually. A full factorial design (FFD) with a central point combined with response surface methodology (RSM) was employed to optimize the experimental variables, including solution pH, applied current, and anode material. The optimized EF conditions involved a pH of 4.0, a current of 100 mA, and an SD-TNT anode for 120 min of electrolysis. Under these conditions, the MB solution achieved complete decolorization and 45% of total organic carbon (TOC) removal after 120 min of EF treatment. The findings indicate that the hydroxyl radical (•OH) plays a crucial role as the primary oxidizing agent in the EF process. The decay of MB followed pseudo-first-order kinetics, reflecting a consistent formation of •OH radicals that effectively attacked the MB dye and its subproducts during mineralization. Moreover, the EF process exhibited superior performance in terms of energy consumption (EC) and mineralization current efficiency (ECM) in the initial treatment stages, while the presence of recalcitrant by-products and loss of anode self-doping impacted performance in the later stages. The optimized EF conditions and the understanding gained from this study contribute to the advancement of sustainable wastewater treatment strategies for the removal of organic dyes.

Reiki for promotion of health and sleep quality in hospital nursing professionals.

OBJECTIVES: to know the repercussions of a Reiki therapy intervention on the sleep quality of nursing professionals working in a general hospital. METHODS: a qualitative-quantitative study conducted with 16 professionals from the nursing team of a hospital in northwest Paraná, who participated in an intervention consisting of six weekly Reiki sessions. Data collected from September, 2019 to March, 2020 through semi-structured interviews and application of the Pittsburgh Sleep Quality Index before and after the intervention. RESULTS: better sleep quality, characterized by a reduction in the time to fall asleep and in nightmares, and an increase in sleeping hours. CONCLUSIONS: reiki intervention had a positive impact on the sleep quality of participants.

Gene function prediction in five model eukaryotes exclusively based on gene relative location through machine learning.

The function of most genes is unknown. The best results in automated function prediction are obtained with machine learning-based methods that combine multiple data sources, typically sequence derived features, protein structure and interaction data. Even though there is ample evidence showing that a gene's function is not independent of its location, the few available examples of gene function prediction based on gene location rely on sequence identity between genes of different organisms and are thus subjected to the limitations of the relationship between sequence and function. Here we predict thousands of gene functions in five model eukaryotes (Saccharomyces cerevisiae, Caenorhabditis elegans, Drosophila melanogaster, Mus musculus and Homo sapiens) using machine learning models exclusively trained with features derived from the location of genes in the genomes to which they belong. Our aim was not to obtain the best performing method to automated function prediction but to explore the extent to which a gene's location can predict its function in eukaryotes. We found that our models outperform BLAST when predicting terms from Biological Process and Cellular Component Ontologies, showing that, at least in some cases, gene location alone can be more useful than sequence to infer gene function.

Reiki for promotion of health and sleep quality in hospital nursing professionals / Reiki para la promoción de la salud y la calidad de sueño en profesionales de enfermería hospitalaria / Reiki para promoção da saúde e qualidade do sono em profissionais de enfermagem de hospital

ABSTRACT Objectives: to know the repercussions of a Reiki therapy intervention on the sleep quality of nursing professionals working in a general hospital. Methods: a qualitative-quantitative study conducted with 16 professionals from the nursing team of a hospital in northwest Paraná, who participated in an intervention consisting of six weekly Reiki sessions. Data collected from September, 2019 to March, 2020 through semi-structured interviews and application of the Pittsburgh Sleep Quality Index before and after the intervention. Results: better sleep quality, characterized by a reduction in the time to fall asleep and in nightmares, and an increase in sleeping hours. Conclusions: reiki intervention had a positive impact on the sleep quality of participants.

RESUMEN Objetivos: conocer las repercusiones de una intervención con sesiones de Reiki en la calidad del sueño de los profesionales de enfermería que actúan en un hospital general. Métodos: estudio cualitativo-cuantitativo realizado con 16 profesionales del equipo de enfermería de un hospital del noroeste de Paraná que participaron de una intervención de seis sesiones semanales de Reiki. Datos recolectados de septiembre, 2019 a marzo, 2020 a través de entrevistas semiestructuradas y aplicación del Índice de Calidad de Sueño de Pittsburgh antes y después de la intervención. Resultados: mejor calidad de sueño, caracterizada por una reducción del tiempo para conciliar el sueño y de las pesadillas y un aumento de las horas de sueño. Conclusiones: la intervención de Reiki tuvo un impacto positivo en la calidad de sueño de los participantes.

RESUMO Objetivos: conhecer as repercussões de intervenção com sessões de reiki na qualidade do sono de profissionais de enfermagem atuantes em um hospital geral. Métodos: estudo de abordagem quali-quantitativa realizado com 16 profissionais da equipe de enfermagem de um hospital no noroeste do Paraná que participaram de uma intervenção constituída por seis sessões semanais de reiki. Dados coletados de setembro/2019 a março/2020, mediante entrevista semiestruturada e aplicação do Índice de Qualidade do Sono de Pittsburgh antes e após o término da intervenção. Resultados: melhora na qualidade do sono, caracterizada por redução do tempo para adormecer e dos pesadelos e aumento nas horas dormidas. Conclusões: a intervenção com reiki repercutiu de forma positiva sobre a qualidade do sono das participantes.

Características clínicas y epidemiológicas de las neoplasias mieloproliferativas Philadelphia negativas en el sistema público de salud de Chile / Clinical and epidemiological characteristics of the Philadelphia negative myeloproliferative neoplasms in Chile

BACKGROUND: Philadelphia-negative myeloproliferative neoplasms (Ph-MPN) are chronic hematological disorders characterized by the overproduction of one or more mature myeloid blood cell lineages. Classical Ph-MPN are polycythemia vera (PV), essential thrombocytopenia (ET) and primary myelofibrosis (PMF). AIM: To assess the epidemiological, clinical and diagnostic characteristics of Ph-MPN in Chile. Material and Methods: Retrospective review of medical records of all patients referred as MPN from 2012 to 2017. Patients with (9;21) translocation were excluded. RESULTS: Data of 462 cases with a median age of 69 years from 10 public hospitals was reviewed. ET was the most frequently Ph-MNP found. The incidence of Ph-MPN was 1.5 x 100.000 cases. The JAK2 V617F mutation study was performed in 96% of patients and only 30% had a bone marrow biopsy. Thrombotic events were observed in 29% of patients. Bleeding events were observed in 7%. Five-year overall survival was 87%. Conclusions: ET is the most frequent Ph-MPN. The mean incidence was lower than reported in the literature, in part because of a sub diagnosis.

Real-World Data on Adult T-Cell Leukemia/Lymphoma in Latin America: A Study From the Grupo de Estudio Latinoamericano de Linfoproliferativos.

PURPOSE: Adult T-cell leukemia/lymphoma (ATLL) is an aggressive disease caused by the human T-cell leukemia virus type 1. Real-world data of ATLL in Latin America are lacking. PATIENTS AND METHODS: We analyzed patients with ATLL (acute, lymphomatous, chronic, and smoldering) encountered in 11 Latin American countries between 1995 and 2019. Treatment response was assessed according to the 2009 consensus report. Survival curves were estimated using the Kaplan-Meier method and log-rank test. RESULTS: We identified 253 patients; 226 (lymphomatous: n = 122, acute: n = 73, chronic: n = 26, and smoldering: n = 5) had sufficient data for analysis (median age 57 years). Most patients with ATLL were from Peru (63%), Chile (17%), Argentina (8%), and Colombia (7%). Hypercalcemia was positively associated with acute type (57% v lymphomatous 27%, P = .014). The median survival times (months) were 4.3, 7.9, 21.1, and not reached for acute, lymphomatous, chronic, and smoldering forms, with 4-year survival rates of 8%, 22%, 40%, and 80%, respectively. First-line zidovudine (AZT)-interferon alfa (IFN) resulted in an overall response rate of 63% (complete response [CR] 24%) for acute. First-line chemotherapy yielded an overall response rate of 41% (CR 29%) for lymphomatous. CR rate was 42% for etoposide, cyclophosphamide, vincristine, doxorubicin, and prednisone versus 12% for cyclophosphamide, vincristine, doxorubicin, and prednisone-like regimen (P < .001). Progression-free survival at 1 year for acute type patients treated with AZT-IFN was 67%, whereas 2-year progression-free survival in lymphomatous type patients who achieved CR after chemotherapy was 77%. CONCLUSION: This study confirms Latin American ATLL presents at a younger age and has a high incidence of lymphomatous type, low incidence of indolent subtypes, and worse survival rates as compared with Japanese patients. In aggressive ATLL, chemotherapy remains the preferred choice for lymphomatous favoring etoposide-based regimen (etoposide, cyclophosphamide, vincristine, doxorubicin, and prednisone), whereas AZT-IFN remains a good first-line option for acute subtype.

Chronotype, general health and quality of sleep in a population of Spanish nurses.

OBJECTIVE: The aim of this study was to investigate the association between chronotype, general health status and sleep quality in a sample of Spanish nurses. METHOD: An observational study assessing morningness-eveningness predisposition, general health status and quality of sleep was conducted between January and April 2018. Univariate and multivariate analyses were performed. A linear regression model was carried out to determine the influence of the variables on the morningness-eveningness type. RESULTS: Morning-type was associated with aging (ß = 0.249, p = 0.005), being married (ß = 3.970, p = 0.033), and with a self-assessed low daily sleepiness (ß = -0.311, p = 0.152). Good quality of sleep was moderately correlated with high values of general health (r = 0.337) and perceived quality of life (r = 0.426). CONCLUSION: Anomalies of the circadian rhythm together with the features of shift workers may play an important role in predicting self-assessed general health status or the quality of sleep in nurses.

[Clinical and epidemiological characteristics of the Philadelphia negative myeloproliferative neoplasms in Chile]. / Características clínicas y epidemiológicas de las neoplasias mieloproliferativas Philadelphia negativas en el sistema público de salud de Chile.

BACKGROUND: Philadelphia-negative myeloproliferative neoplasms (Ph-MPN) are chronic hematological disorders characterized by the overproduction of one or more mature myeloid blood cell lineages. Classical Ph-MPN are polycythemia vera (PV), essential thrombocytopenia (ET) and primary myelofibrosis (PMF). AIM: To assess the epidemiological, clinical and diagnostic characteristics of Ph-MPN in Chile. MATERIAL AND METHODS: Retrospective review of medical records of all patients referred as MPN from 2012 to 2017. Patients with (9;21) translocation were excluded. RESULTS: Data of 462 cases with a median age of 69 years from 10 public hospitals was reviewed. ET was the most frequently Ph-MNP found. The incidence of Ph-MPN was 1.5 x 100.000 cases. The JAK2 V617F mutation study was performed in 96% of patients and only 30% had a bone marrow biopsy. Thrombotic events were observed in 29% of patients. Bleeding events were observed in 7%. Five-year overall survival was 87%. CONCLUSIONS: ET is the most frequent Ph-MPN. The mean incidence was lower than reported in the literature, in part because of a sub diagnosis.

[Clinical and epidemiological characteristics of the Philadelphia negative myeloproliferative neoplasms in Chile]. / Características clínicas y epidemiológicas de las neoplasias mieloproliferativas Philadelphia negativas en el sistema público de salud de Chile.

BACKGROUND: Philadelphia-negative myeloproliferative neoplasms (Ph-MPN) are chronic hematological disorders characterized by the overproduction of one or more mature myeloid blood cell lineages. Classical Ph-MPN are polycythemia vera (PV), essential thrombocytopenia (ET) and primary myelofibrosis (PMF). AIM: To assess the epidemiological, clinical and diagnostic characteristics of Ph-MPN in Chile. MATERIAL AND METHODS: Retrospective review of medical records of all patients referred as MPN from 2012 to 2017. Patients with (9;21) translocation were excluded. RESULTS: Data of 462 cases with a median age of 69 years from 10 public hospitals was reviewed. ET was the most frequently Ph-MNP found. The incidence of Ph-MPN was 1.5 x 100.000 cases. The JAK2 V617F mutation study was performed in 96% of patients and only 30% had a bone marrow biopsy. Thrombotic events were observed in 29% of patients. Bleeding events were observed in 7%. Five-year overall survival was 87%. CONCLUSIONS: ET is the most frequent Ph-MPN. The mean incidence was lower than reported in the literature, in part because of a sub diagnosis.

Technosociality in the daily lives of primary care professionals and health promotion: scoping review / A tecnossocialidade no cotidiano de profissionais da atenção primária e promoção da saúde: scoping review

ABSTRACT This study aimed to map the available evidence on technosociality in the daily lives of primary care professionals to promote the health of people/families. This is a scoping review based on the Joanna Briggs Institute and Prisma-ScR. The PCC strategy (participants, concept and context) was used. The sample comprises 25 articles. The predominant language was English, the year, 2018, the strength of evidence, 5 and the quality of evidence, high and moderate. Technologies are part of the daily life of PHC professionals and include health control, curative and medication measures. They are sources of communication between professionals and professionals and users. For the use of technologies, it is essential that professionals are trained, using evidence-based practices to achieve the objectives in view of the needs of each one. The use of technologies to promote the health of people/families represents a technological innovation in Primary Health Care, with the possibility of impacting health conditions and promoting healthier life choices.

RESUMO Este estudo teve por objetivo mapear a evidência disponível sobre a tecnossocialidade no cotidiano de profissionais da atenção primária para a promoção da saúde de pessoas/famílias. Trata-se de uma scoping review fundamentada no Joanna Briggs Institute e no Prisma-ScR. Foi utilizada a estratégia PCC (participants, concept e context). A amostra compõe 25 artigos. O idioma predominante foi o inglês, o ano foi 2018, a força de evidência 5 e a qualidade de evidência alta e moderada. As tecnologias fazem parte do cotidiano dos profissionais da APS e abrangem medidas de controle da saúde, curativas e medicamentosas. São fontes de comunicação entre profissionais e profissionais e usuários. Para o uso de tecnologias, é imprescindível que os profissionais estejam capacitados, utilizando práticas baseadas em evidências para alcançar os objetivos perante as necessidades de cada um. O uso de tecnologias para a promoção da saúde de pessoas/ famílias representa uma inovação tecnológica na Atenção Primária à Saúde, com possibilidade de impacto nas condições de saúde e promoção de escolhas mais saudáveis de vida.

Chronotype, general health and quality of sleep in a population of Spanish nurses / Cronotipo, salud general y calidad del sueño en una población de enfermeros españoles / Cronótipo, saúde geral e qualidade do sono em uma população de enfermeiros espanhóis

ABSTRACT Objective The aim of this study was to investigate the association between chronotype, general health status and sleep quality in a sample of Spanish nurses. Method An observational study assessing morningness-eveningness predisposition, general health status and quality of sleep was conducted between January and April 2018. Univariate and multivariate analyses were performed. A linear regression model was carried out to determine the influence of the variables on the morningness-eveningness type. Results Morning-type was associated with aging (β = 0.249, p = 0.005), being married (β = 3.970, p = 0.033), and with a self-assessed low daily sleepiness (β = -0.311, p = 0.152). Good quality of sleep was moderately correlated with high values of general health (r = 0.337) and perceived quality of life (r = 0.426). Conclusion Anomalies of the circadian rhythm together with the features of shift workers may play an important role in predicting self-assessed general health status or the quality of sleep in nurses.

RESUMEN Objetivo Este estudio tuvo como objetivo investigar la asociación entre el cronotipo, el estado general de salud y la calidad del sueño en una muestra de enfermeras españolas. Método Desde enero a abril de 2018, se realizó un estudio observacional para evaluar la predisposición matutina-vespertina, el estado de salud general y la calidad del sueño. Se realizaron análisis univariante y multivariante. Se efectuó un modelo de regresión lineal para determinar la influencia de las variables en el tipo matutino-vespertino. Resultados El tipo matutino se asoció con el envejecimiento (β = 0,249; p = 0,005), estar casado (β = 3,970; p = 0,033) y con un bajo nivel de somnolencia diaria autoevaluada (β = -0,311; p = 0,152). La buena calidad del sueño se correlacionó moderadamente con valores altos de salud general (r = 0,337) y de calidad de vida percibida (r = 0,426). Conclusión Las anomalías del ritmo circadiano junto con las características de los trabajadores por turnos pueden desempeñar un papel importante en la predicción del estado de salud general autoevaluado o la calidad del sueño de las enfermeras.

RESUMO Objetivo O objetivo deste estudo foi investigar a associação entre cronótipo, estado geral de saúde e qualidade do sono em uma amostra de enfermeiras espanholas. Método Foi realizado um estudo observacional entre janeiro e abril de 2018 que avaliou a predisposição matutina-vespertina, o estado geral de saúde e a qualidade do sono. Foram realizadas análises univariadas e multivariadas. Um modelo de regressão linear foi realizado para determinar a influência das variáveis sobre o tipo matutino-vespertino. Resultados O tipo matinal foi associado ao envelhecimento (β = 0,249, p = 0,005), ser casado (β = 3,970, p = 0,033) e com baixa sonolência diária autoavaliada (β = -0,311, p = 0,152). Boa qualidade de sono foi moderadamente correlacionada com altos valores de saúde geral (r = 0,337) e qualidade de vida percebida (r = 0,426). Conclusão Anomalias do ritmo circadiano em conjunto com as características dos trabalhadores em turnos podem desempenhar um papel importante na previsão do estado geral de saúde autoavaliado ou da qualidade do sono em enfermeiras.

Quotidiano de equipes de consultório na rua: tecendo redes para a promoção da saúde / El día a día de los equipos consultores de calle: tejiendo redes para la promoción de la salud / The routine of the street outreach office team: weaving networks for health promotion

Resumo Objetivo compreender as potências e limites para a promoção da saúde de pessoas em situação de rua no quotidiano da equipe de Consultório na Rua. Método estudo de casos múltiplos holístico-qualitativo, fundamentado na Sociologia Compreensiva do Quotidiano, realizado com dois informantes-chave e 20 profissionais de equipe de Consultório na Rua de duas capitais do Sul do Brasil. Utilizou-se a Análise de Conteúdo Temática. Resultados as potências para a promoção da saúde de pessoas em situação de rua advêm da articulação da rede intrasetorial e intersetorial pela equipe de Consultório na Rua, além do vínculo e da redução de danos. Os limites enfrentados para desenvolver ações de promoção da saúde se deparam na especificidade e características desse público na terceirização da assistência social, na gestão, na política. Conclusões e implicações para a prática a promoção da saúde ainda é um desafio para a equipe a ser superado perante as demandas de agravos, o tratamento do adoecimento e a lógica biomédica. As estratégias intrasetoriais e intersetoriais podem ser estabelecidas para alcançar ações em rede e efetivamente promover saúde, contemplando direitos fundamentais à vida de pessoas em situação de rua, mesmo em um quotidiano com condições desfavoráveis de viver e conviver.

Resumen Objetivo comprender los poderes y límites para promover la salud de las personas que viven en las calles en la vida cotidiana del equipo de Consultório na Rua. Método estudio de caso múltiple holístico-cualitativo, basado en la Sociología Comprensiva del Cotidiano, realizado con dos informantes clave y 20 profesionales del equipo de Consultório na Rua de dos capitales en el sur de Brasil. Se utilizó el análisis de contenido temático. Resultados las competencias para promover la salud de las personas que viven en la calle provienen de la articulación de la red intrasectorial e intersectorial por parte del equipo Consultório na Rua, además del vínculo y la reducción de daños. Los límites que se enfrentan para desarrollar acciones de promoción de la salud se enfrentan en la especificidad y características de este público, en la externalización de la asistencia social, en la gestión, en la política. Conclusiones e implicaciones para la práctica la promoción de la salud sigue siendo un desafío para el equipo a superar ante los problemas de salud, el tratamiento de la enfermedad y la lógica biomédica. Se pueden establecer estrategias intrasectoriales e intersectoriales para lograr acciones en red y promover eficazmente la salud, contemplando los derechos fundamentales a la vida de las personas que viven en la calle, incluso en una vida cotidiana con condiciones desfavorables para vivir y convivir.

Abstract Objective to understand the powers and limits for promoting the health of homeless people in the daily life of the Outreach office team. Method a holistic-qualitative multiple case study, based on Comprehensive Sociology of Everyday Life, conducted with two key informants and 20 outreach office team professionals from two Southern Brazilian Capitals. We used the technique of thematic content analysis. Results the powers for health promotion of homeless people come from the articulation of the intrasectoral and intersectoral network by the Outreach office team, in addition to bonding and harm reduction. The limits faced to develop health promotion actions are faced in the specificity and characteristics of this public, in the outsourcing of social assistance, in management, in politics. Conclusions and implications for practice health promotion is still a challenge for the team to be overcome before demands of injuries, treatment of illness and biomedical logic. Intrasectoral and intersectoral strategies can be established to achieve network actions and effectively promote health, contemplating fundamental rights to the lives of homeless people, even in a daily life with unfavorable conditions of living and living.

Different outcomes for transplant-eligible newly diagnosed multiple myeloma patients in Latin America according to the public versus private management: a GELAMM study.

The aim of this study was to describe clinical and survival characteristics of transplant-eligible multiple myeloma (MM) patients in Latin America (LA), with a special focus on differences between public and private healthcare facilities. We included 1293 patients diagnosed between 2010 and 2018. A great disparity in outcomes and survival between both groups was observed. Late diagnosis and low access to adequate frontline therapy and ASCT in public institutions probably explain these differences. Patients treated with novel drug induction protocols, followed by autologous stem cell transplantation (ASCT) and maintenance, have similar overall survival compared to that published internationally.

Resultados de la artrolisis artroscópica seguida de un programa de rehabilitación precoz ambulatoria con catéter interescalénico permanente como tratamiento de la rigidez secundaria de hombro / Arthroscopic arthrolysis followed by an early rehabilitation protocol using an indwelling interscalene catheter as treatment of secondary shoulder stiffness

Introducción: Evaluar los resultados de la artrolisis artroscópica seguida de un protocolo de rehabilitación acelerada empleando un catéter interescalénico permanente para tratar rigideces secundarias de hombro.Material y métodos: Revisión retrospectiva de casos intervenidos de artrolisis de hombro con alguna causa identificable de la rigidez. Se emplea un catéter interescalénico para bloqueo nervioso de la extremidad afecta que se mantiene al menos 5 días. El alta hospitalaria se realiza después de la primera sesión de rehabilitación en las siguientes 24 horas tras la intervención. Se evalúa variación de la movilidad articular, mejoría del dolor y de la capacidad funcional.Resultado: Se incluyen 11 pacientes en el estudio. Las causas de la rigidez fueron cirugías previas de osteosíntesis de fracturas de húmero proximal, reparaciones de manguito rotador, reparación de Bankart y traumatismos sin fractura. El seguimiento medio fue de 26.4 (8.3) meses. Se observa una mejoría estadísticamente significativa en la flexión [95.6 (32)º - 153.4 (59)º], rotación externa [33.9 (17)º - 59.2 (32)º], EVA [7.2 (2.9) - 2.1 (1.7)] y Quick-DASH Score [68.2 (33)º - 22.7 (13)º], pero no en la rotación interna ni en la ASES Score. No se registró ninguna complicación. Un paciente precisó una nueva artrolisis por no mejoría de movilidad a los 3 meses. La causa de la rigidez no influyó en los resultados.Conclusión: La artrolisis artroscópica seguida del inicio precoz de la fisioterapia con bloqueo de la extremidad con catéter interescalénico permanente es segura y proporciona buenos resultados funcionales. Tipo de estudio: Serie de Casos. Nivel de Evidencia: IV

Objective: To evaluate outcomes of arthroscopic arthrolysis followed by an early rehabilitation protocol using an indwelling interscalene catheter as treatment of secondary shoulder stiffness.Materials and methods: A retrospective review of cases who had surgery for shoulder stiffness with an identifiable cause is performed. An interscalene catheter is left after surgery for nervous blockade for at least 5 days. Hospital check-out is carried out immediately after first physical therapy session, at 24 hours from surgery. Outcomes are presented as improvement of range of motion, pain and functional status.Results: 11 patients were included in the study. Causes of stiffness were previous surgeries consisting on osteosynthesis for proximal humeral fractures, repair of rotator cuff tears, Bankart repair and trauma without fracture. Mean follow-up was 26.4 (8.3) months. Significant differences on flexion [95.6 (32)º - 153.4 (59)º], external rotation [33.9 (17)º - 59.2 (32)º], VAS [7.2 (2.9) - 2.1 (1.7)] and Quick-DASH Score [68.2 (33)º - 22.7 (13)º] were observed, while no differences on internal rotation and ASES Score. No complications were registered. One case required re-operation at 3 months due to no progression of shoulder motion. Cause of stiffness did not influence outcomes.Conclusion: Arthroscopic arthrolysis followed by early rehabilitation with nervous blockade of the extremity by an indwelling interscalene catheter is safe and provides good functional outcomes. Study type: case report. Level of evidence: IV

An improved catalogue of putative synaptic genes defined exclusively by temporal transcription profiles through an ensemble machine learning approach.

BACKGROUND: Assembly and function of neuronal synapses require the coordinated expression of a yet undetermined set of genes. Previously, we had trained an ensemble machine learning model to assign a probability of having synaptic function to every protein-coding gene in Drosophila melanogaster. This approach resulted in the publication of a catalogue of 893 genes which we postulated to be very enriched in genes with a still undocumented synaptic function. Since then, the scientific community has experimentally identified 79 new synaptic genes. Here we use these new empirical data to evaluate our original prediction. We also implement a series of changes to the training scheme of our model and using the new data we demonstrate that this improves its predictive power. Finally, we added the new synaptic genes to the training set and trained a new model, obtaining a new, enhanced catalogue of putative synaptic genes. RESULTS: The retrospective analysis demonstrate that our original catalogue was significantly enriched in new synaptic genes. When the changes to the training scheme were implemented using the original training set we obtained even higher enrichment. Finally, applying the new training scheme with a training set including the 79 new synaptic genes, resulted in an enhanced catalogue of putative synaptic genes. Here we present this new catalogue and announce that a regularly updated version will be available online at: http://synapticgenes.bnd.edu.uy CONCLUSIONS: We show that training an ensemble of machine learning classifiers solely with the whole-body temporal transcription profiles of known synaptic genes resulted in a catalogue with a significant enrichment in undiscovered synaptic genes. Using new empirical data provided by the scientific community, we validated our original approach, improved our model an obtained an arguably more precise prediction. This approach reduces the number of genes to be tested through hypothesis-driven experimentation and will facilitate our understanding of neuronal function. AVAILABILITY: http://synapticgenes.bnd.edu.uy.

Mieloma múltiple en Chile: Respuesta a tratamiento en pacientes con mieloma múltiple elegibles para trasplante autólogo de progenitores hematopoyéticos / Response rates to first-line treatment in eligible patients to autologous stem transplantation in Chile

Background The treatment of choice of newly diagnosed multiple myeloma (NDMM) is an induction with proteasome inhibitors followed autologous stem cell transplantation (HSCT). Since 2013, the treatment of these patients in the public system is based on CTD (cyclophosphamide, thalidomide, and dexamethasone). Aim To evaluate the response rates achieved with CTD, and the results of HSCT in patients with NDMM in the public setting. Material and Methods Data from patients considered as candidates for HSCT from different centers of the National Adult Antineoplastic Drug Program (PANDA, for its acronym in Spanish), diagnosed between 2013 and 2017, was analyzed. The response to treatment of first and second lines of treatment was evaluated, in addition to the results of HSCT. An optimal Response was defined as the sum of strict complete remission, complete remission and very good partial response (sCR, CR and VGPR). Results One hundred and seventy-seven patients were analyzed, 54% women, and 53% with IgG multiple myeloma. Information about the international staging system was retrieved in 127 patients (71%). Seventeen percent were ISS I, 22% in ISS II and 32% ISS III. CTD was used as first treatment in 106 patients (60%), and cyclophosphamide, bortezomib and dexamethasone (CyBorD) in 13 (7%). As first line, CTD had an overall response of 50.9%, and CyBorD of 76.9%. Thirty patients were treated with bortezomib as second line treatment. Forty patients (22%) underwent HSCT. The 5-year Overall Survival (OS) in transplanted patients and non-transplanted patients was 100 and 62% respectively (p < 0.01). Conclusions The response rate achieved by CTD in these patients is suboptimal. The response to CyBorD was better.

AL amyloidosis in the Chilean public health system: a pending debt: multicenter study of the Chilean Monoclonal Gammopathies Cooperative Group / Amiloidosis AL en el sistema público de Chile: una deuda pendiente: estudio multicéntrico

ABSTRACT Background: Immunoglobulin light chain (AL) amyloidosis is a rare and underdiagnosed entity. Aim: To characterize patients with AL amyloidosis in Chilean public health centers. Material and Methods: We conducted a retrospective, multicenter study. Public centers of the Chilean Monoclonal Gammopathies Cooperative Group were asked to search for patients with AL amyloidosis in their databases. Epidemiological, clinical and laboratory characteristics were evaluated. Results: Forty-two patients aged 22 to 84 years were found. Twenty four percent had localized AL amyloidosis; 64% had a lambda light chain clone; 47% were associated with multiple myeloma and 9% with non-Hodgkin lymphoma. The most commonly involved organ was the kidney (76%). Serum free light chains were measured in 31% and an echocardiogram was performed in 74% of patients. Seventeen percent of patients received only palliative care, 17% were treated with bortezomib, 21% with thalidomide, and 40% with melphalan. No patient was transplanted. The mean overall survival (OS) of the group was 19 months. The 5-year OS was 28%. Conclusions: It is important to obtain these realistic, national data to initiate strategies to improve early diagnosis and proper management of this disease.

La amiloidosis AL es una entidad poco frecuente y subdiagnosticada. Mientras todo el mundo discute sobre las nuevas herramientas diagnósticas y terapéuticas, en Chile y en América Latina en general, estamos lejos de esa realidad. El objetivo del presente estudio fue caracterizar a los pacientes con amiloidosis AL en centros del sistema público de nuestro país. Se realizó un estudio retrospectivo, multicéntrico, descriptivo. Los centros públicos del grupo cooperativo hematológico chileno buscaron en sus bases de datos pacientes diagnosticados con amiloidosis AL. Se evaluaron las características epidemiológicas, clínicas y de laboratorio. La edad media fue de 65 años. A 24% de los pacientes se les diagnosticó amiloidosis AL localizada; 64% tuvo paraproteína con cadena ligera lambda; 47% se asoció con mieloma múltiple y 9% con linfoma no Hodgkin. El órgano afectado con mayor frecuencia fue el riñón (76%). Las cadenas ligeras libres de suero se realizaron en 31% y ecocardiograma en 74%. El 17% recibió solo cuidados paliativos, 17% recibió tratamiento con bortezomib, 21% con talidomida y 40% con melfalán. Ningún paciente fue trasplantado. La media de sobrevida global (SG) del grupo fue de 19 meses. La SG a 5 años fue de 28%. Es importante reportar estos resultados nacionales para iniciar estrategias que mejoren tanto el diagnóstico temprano como el tratamiento de esta patología. Por lo tanto, mejorar la sospecha diagnóstica es crucial.