Correction: Label free localization of nanoparticles in live cancer cells using spectroscopic microscopy.

Correction for 'Label free localization of nanoparticles in live cancer cells using spectroscopic microscopy' by Graham L. C. Spicer et al., Nanoscale, 2018, 10, 19125-19130, https://doi.org/10.1039/C8NR07481J.

Extended depth of focus by self-imaging wavefront division with the mirror tunnel.

The mirror tunnel is a component used to extend the depth of focus for compact imaging probes used in endoscopic optical coherence tomography (OCT). A fast and accurate method for mirror tunnel probe simulation, characterization, and optimization is needed, with the aim of reconciling wave- and ray-optics simulation methods and providing a thorough description of the physical operating principle of the mirror tunnel. BeamLab software, employing the beam propagation method, was used to explore the parameter space and quantify lateral resolution and depth of focus extension. The lateral resolution performance was found to depend heavily on the metric chosen, implying that care should be taken in the interpretation of optimization and simulation results. Interpreting the mirror tunnel exit face as an extended object gives an understanding of the probe operation, decoupling it from the focusing optics and potentially helping to reduce the parameter space for future optimization.

Label free localization of nanoparticles in live cancer cells using spectroscopic microscopy.

Gold nanoparticles (GNPs) have become essential tools used in nanobiotechnology due to their tunable plasmonic properties and low toxicity in biological samples. Among the available approaches for imaging GNPs internalized by cells, hyperspectral techniques stand out due to their ability to simultaneously image and perform spectral analysis of GNPs. Here, we present a study utilizing a recently introduced hyperspectral imaging technique, live-cell PWS, for the imaging, tracking, and spectral analysis of GNPs in live cancer cells. Using principal components analysis, the extracellular or intracellular localization of the GNPs can be determined without the use of exogenous labels. This technique uses wide-field white light, assuring minimal toxicity and suitable signal-to-noise ratio for spectral and temporal resolution of backscattered signal from GNPs and local cellular structures. The application of live-cell PWS introduced here could make a great impact in nanomedicine and nanotechnology by giving new insights into GNP internalization and intracellular trafficking.

Detection of extracellular matrix modification in cancer models with inverse spectroscopic optical coherence tomography.

In cancer biology, there has been a recent effort to understand tumor formation in the context of the tissue microenvironment. In particular, recent progress has explored the mechanisms behind how changes in the cell-extracellular matrix ensemble influence progression of the disease. The extensive use of in vitro tissue culture models in simulant matrix has proven effective at studying such interactions, but modalities for non-invasively quantifying aspects of these systems are scant. We present the novel application of an imaging technique, Inverse Spectroscopic Optical Coherence Tomography, for the non-destructive measurement of in vitro biological samples during matrix remodeling. Our findings indicate that the nanoscale-sensitive mass density correlation shape factor D of cancer cells increases in response to a more crosslinked matrix. We present a facile technique for the non-invasive, quantitative study of the micro- and nano-scale structure of the extracellular matrix and its host cells.