Assuntos
Acetilglucosamina/análogos & derivados , Antifúngicos/farmacologia , Quitina/metabolismo , Compostos de Dansil/metabolismo , Corantes Fluorescentes/metabolismo , Acetilglucosamina/síntese química , Acetilglucosamina/metabolismo , Candida albicans/efeitos dos fármacos , Candida albicans/metabolismo , Parede Celular/efeitos dos fármacos , Quitina Sintase/antagonistas & inibidores , Compostos de Dansil/síntese química , Avaliação Pré-Clínica de Medicamentos , Inibidores Enzimáticos/química , Inibidores Enzimáticos/farmacologia , Corantes Fluorescentes/química , Nucleosídeos de Pirimidina/farmacologiaRESUMO
During the course of a screening program for inhibitors of myo-inositol monophosphatase we fermented the strain ATCC 20928, a known producer of L-671,776. We now show that this strain produces a complex of at least three sesquiterpenic compounds, L-671,776 (termed factor B) and two structurally related substances, termed factors A and C. Both factors A and C, like L-671,776, exhibited inhibitory activity against myo-inositol monophosphatase. Six other fungi producing the above mentioned compounds were also isolated and taxonomically characterized.
Assuntos
Benzofuranos/química , Inibidores Enzimáticos/química , Inibidores Enzimáticos/farmacologia , Fungos Mitospóricos/metabolismo , Monoéster Fosfórico Hidrolases/antagonistas & inibidores , Compostos de Espiro/química , Benzofuranos/metabolismo , Benzofuranos/farmacologia , Cromatografia Líquida de Alta Pressão , Meios de Cultura , Inibidores Enzimáticos/isolamento & purificação , Fermentação , Fungos Mitospóricos/química , Fungos Mitospóricos/classificação , Compostos de Espiro/metabolismo , Compostos de Espiro/farmacologia , Stachybotrys/química , Stachybotrys/classificação , Stachybotrys/metabolismoAssuntos
Autoanálise/métodos , Avaliação Pré-Clínica de Medicamentos/métodos , Inibidores Enzimáticos/farmacologia , Monoéster Fosfórico Hidrolases/antagonistas & inibidores , Monoéster Fosfórico Hidrolases/metabolismo , Avaliação Pré-Clínica de Medicamentos/instrumentação , Fosfatos/metabolismo , Microbiologia do Solo , Solventes , Stachybotrys/metabolismo , Fatores de Tempo , TitulometriaRESUMO
The principle of equivalent light action predicts that two light treatments (wavelengths ;lambda(1) and lambda(2)) producing the same Pfr/P ratio (phi(lambda1) = phi(lambda2)) and the same rate of phytochrome photoconversion (k(lambda1) = k(lambda2)) are perceived by phytochrome as being the same and should produce the same effect. The results of experiments based on the principle of equivalent light action indicate that cryptochrome is involved in the photoregulation of anthocyanin production elicited by blue light in tomato seedlings. This was also the case for one strain of cabbage seedlings. For another strain of cabbage seedlings, the results suggest that cryptochrome is either not involved or that the state of phytochrome is the principal limiting factor.