C-Phycocyanin ameliorates 2-acetylaminofluorene induced oxidative stress and MDR1 expression in the liver of albino mice.

AIM: To study the effect of C-Phycocyanin (C-PC), a biliprotein isolated from Spirulina platensis, on 2-acetylaminofluorene (2-AAF) induced oxidative stress and MDR1 expression in the liver of albino mice. METHODS: In the present study, albino mice aged 40-60 days were used. The mice were randomly assigned to four groups of six animals each. The first group was treated with the vehicle (absolute alcohol), the second group was treated with C-PC (50 mg/kg body weight), the third group was treated with 2-AAF (25 mg/kg body weight) and the fourth group was treated with C-PC (50 mg/kg body weight) and 2-AAF, daily for 3 days. The mice were sacrificed and the tissues were collected and stored for histology and biochemical studies. RESULTS: 2-AAF induced liver tissue damage in albino mice. 2-AAF treatment resulted in upregulation of MDR1 expression and enhanced the generation of reactive oxygen species (ROS). It also induced phosphorylation of Akt and nuclear translocation of NF-kappaB. Co-administration of C-PC and 2-AAF inhibited the expression of MDR1 by preventing ROS generation, Akt phosphorylation and NF-kappaB nuclear translocation. CONCLUSION: 2-AAF-induced oxidative stress is reduced by C-PC treatment. C-PC inhibited the 2-AAF induced expression of MDR1 by interfering at the level of ROS generation, Akt phosphorylation and NF-kappaB translocation. This study reveals the usefulness of C-PC in preventing oxidative stress and downregulation of MDR1 induced by xenobiotics like 2-AAF.

Betanin a betacyanin pigment purified from fruits of Opuntia ficus-indica induces apoptosis in human chronic myeloid leukemia Cell line-K562.

Betalains are water-soluble nitrogenous vacuolar pigments present in flowers and fruits of many caryophyllales with potent antioxidant properties. In the present study the antiproliferative effects of betanin, a principle betacyanin pigment, isolated from the fruits of Opuntia ficus-indica, was evaluated on human chronic myeloid leukemia cell line (K562). The results show dose and time dependent decrease in the proliferation of K562 cells treated with betanin with an IC(50) of 40 microM. Further studies involving scanning and transmission electron microscopy revealed the apoptotic characteristics such as chromatin condensation, cell shrinkage and membrane blebbing. Agarose electrophoresis of genomic DNA of cells treated with betanin showed fragmentation pattern typical for apoptotic cells. Flow cytometric analysis of cells treated with 40 microM betanin showed 28.4% of cells in sub G0/G1 phase. Betanin treatment to the cells also induced the release of cytochrome c into the cytosol, poly (ADP) ribose polymerase (PARP) cleavage, down regulation Bcl-2, and reduction in the membrane potentials. Confocal microscopic studies on the cells treated with betanin suggest the entry of betanin into the cells. These studies thus demonstrate that betanin induces apoptosis in K562 cells through the intrinsic pathway and is mediated by the release of cytochrome c from mitochondria into the cytosol, and PARP cleavage. The antiproliferative effects of betanin add further value to the nutritional characteristics of the fruits of O. ficus-indica.