RESUMO
The repair processes in the pancreas in infected pancreonecrosis after oxytocin (OT) administration were studied in 120 male rats with the body mass of 280-310 g, using light microscopy, histochemistry, electron microscopy and immunocytochemistry (p53 protein detection). Pancreonecrosis was induced by microtraumatizing the pancreatic lobules with bile injection and Staphylococcus aureus suspension infusion. During days 3-14, the intensifying processes of ischemia, acinar and ductal lysis, pancreatocyte dystrophy and death were observed in experimental animals. In paranecrotic areas, acinar discomplexation was found together with the signs of increased secretory activiry in pancreatocytes. Pancreatic tissues were infiltrated with erythrocytes, granulocytes, lymphocytes and macrophages which were actively engaged in phagocytosis. OT was found to create the conditions necessary for adequate realization of the regenerative properties of pancreatocytes, microvessels, cells of fibroblastic line, and macrophages with a concomitant decrease of apoptotic dominant in cellular elements.
Assuntos
Pâncreas/patologia , Pancreatite/patologia , Regeneração/fisiologia , Animais , Histocitoquímica , Linfócitos/imunologia , Linfócitos/fisiologia , Macrófagos/imunologia , Macrófagos/fisiologia , Masculino , Necrose , Ocitocina/farmacologia , Pâncreas/metabolismo , Pâncreas/microbiologia , Pancreatite/etiologia , Pancreatite/metabolismo , Ratos , Staphylococcus aureus , Proteína Supressora de Tumor p53/metabolismoRESUMO
Structures of the pancreas and magnocellular (supraoptic and paraventricular) nuclei of the hypothalamus of adult male rats were studied in conditions of acute pancreatitis at the light and electron microscopic levels. Histo-and organotypic changes in the parenchymatous and stromal elements of the pancreas were analyzed simultaneously with cytological assessment of the state of the nonapeptidergic neurosecretory centers of the hypothalamus. Blockade of the release of hypothalamic nonapeptides was found to occur at the level of axovasal complexes, and this aggravated the outcome of destructive and necrobiotic changes in the pathologically altered organ.
Assuntos
Neurônios/patologia , Pancreatite/patologia , Núcleo Hipotalâmico Paraventricular/patologia , Núcleo Supraóptico/patologia , Doença Aguda , Animais , Bile/química , Contagem de Células , Coledocolitíase/complicações , Modelos Animais de Doenças , Humanos , Masculino , Microscopia Eletrônica de Transmissão/métodos , Neurônios/ultraestrutura , Pancreatite/induzido quimicamente , Núcleo Hipotalâmico Paraventricular/ultraestrutura , Ratos , Núcleo Supraóptico/ultraestrutura , Fatores de TempoRESUMO
The structures of pancreas and hypothalamic magnocellular nuclei (supraoptic and paraventricular) were studied using light and electron microscopes in acute pancreatitis induced experimentally in mature male rats. Histo- and organotypic transformations of the pancreatic parenchymal and stromal elements were analyzed with a simultaneous cytological assessment of the hypothalamic nonapeptidergic neurosecretory centers. A phenomenon of the inhibition of hypothalamic nonapeptide release at the level in axo-vasal complexes was established, that aggravated the outcome of the destructive and necrobiotic processes in pathologically changed organ.
