[C-reactive protein in diagnosis of complications in renal insufficiency and failure]. / C-reaktivní protein v diagnostice komplikací pri ledvinové nedostatecnosti a selhání.

C-reactive protein (CRP) is one of the positive proteins in an acute phase. It is produced in hepatocytes in response to cytokines activity, especially to IL-6. Its increase is the second biggest after significant bacterial and cardiovascular insults. It reaches its peak between 24 and 48 hours. CRP monitoring makes possible monitoring of the intensity of the pathologic process and to control efficiency of treatment measures according to fluctuation of its level. According to its serum values it can reflect a place of inflammation, e.g. in upper or lower airways, urinary tract etc. It helps to distinguish between bacterial and viral inflammations and to identify size of vascular lesions such as acute myocardial infarction, cerebral infarction, decompensation of atherosclerosis. Because of its easy detection and quick elevation CRP has not only a diagnostic importance but also a prognostic one and is a predictor of a risk of atherosclerosis. Although long lasting renal insufficiency (LLRI), renal failure (RF) and regular dialysis treatment (RDT) are indicated to elevate CRP level, authors present proves that adequately treated patient compensated with an adequate dialysis treatment has normal CRP values for a long time in spite of long lasting comorbidities including atherosclerosis. There has been done a long term monitoring of 10 patients with LLRI and 22 patients with RDT. Their CRP was monitored via a turbidimetric method using sets K-Assay made by company Kamya Bio Comp. Elevated CRP in the samples reflects an acute insult such as infection, cardiovascular disease, diabetes decompensation and last but not least quality of a dialysis treatment.

Quantification of histoautoradiographic evidence of DNA repair synthesis in the liver.

Histoautoradiography was used to detect dimethylnitrosamine-induced 3H-thymidine incorporation in vivo into G phase hepatocytes. A description of the standard procedure for counting the grains as well as the mode of mathematical evaluation is presented. The results exhibited higher sensitivity than those in the investigation of the DNA repair synthesis by means of a scintillation counter using the method of detection of hydroxyurea-resistant incorporation of 3H-thymidine. Thus it was possible to simplify the investigation by lowering the number of evaluated cells. A suitable compromise between precision and laboriousness will probably be achieved by counting 20 hepatocytes per animal. In case that there are striking differences between the experimental and the control group, a qualitative conclusion may be drawn even without counting the grains.