RESUMO
AIM: Construction of an immunologic test-system for detection of causative agents of enteropathogenic Yersinia (Yersinia pseudotuberculosis and Y. enterocolitica) by dot-immunoassay. MATERIALS AND METHODS: Nenoparticles of colloid silver sized 5-9 nm were used as a marker of specific antibodies. IgG fraction was isolated from commercial antisera to Y. pseudotuberculosis (Ο:1) and Y. enterocolitica (Ο:3 and Ο:9). Testing of the obtained test-system was carried out on 20 strains of Y.pseudotuberculosis and Y. enterocolitica (10 of each species). RESULTS: Dot-analysis had a specific character and detected enteropathogenic Yersinia at a level of 5-105 - 8.106 CFU/ml (100 - 1000 CFU in sample). Wherein cross-reaction with heterologic studied microorganisms - Escherichia coli, Salmonella typhimurium, Shigella flexneri, Yersina pestis EV - as not observed. A possibility of simultaneous detection and serotyping of Y. enterocolitica is shown, that is necessary for confirmation of their epidemic significance. CONCLUSION: The developed test-systems allow to study micro volumes of the samples under study (1 µ1), are express (1.5 - 2h), highly sensitive and specific, technically simple and do not require the use of high-cost equipment, special training of the staff, may be successfully used in practical healthcar in laboratories with varying equiptment levels.