RESUMO
The influence of SARS-CoV-2 antigen on the cytokine-producing function of immune cells was studied. We observed suppression of the production of proinflammatory cytokines by 11-46% relative to the spontaneous level under the influence of SARS-CoV-2 antigen vaccine simulator, as well as when it was co-administered with cortisol (IL-6 by 1.8 times and IFNγ by 1.57 times) compared with control samples. IL-8 production was reduced by 1.72 times relative to its spontaneous level. IL-8 production was reduced by 1.72 times relative to its spontaneous level. Under conditions of SARS-CoV-2 stimulation with the vaccine antigen in vitro, an increase in the relative scaled expression of the VEGFA gene by 2.16 times relative to the spontaneous level was observed, which can be regarded as a model "cytokine storm" scenario. The obtained experimental data verify the ideas about the pathogenetic mechanisms of the COVID-19 and can contribute to the development of new approaches to the correction of its complications.
Assuntos
COVID-19 , Vacinas , Humanos , Vacinas contra COVID-19 , SARS-CoV-2/genética , Citocinas/genética , Interleucina-8 , COVID-19/prevenção & controle , Antígenos Virais/genética , Fator A de Crescimento do Endotélio Vascular/genéticaRESUMO
Comparative analysis covered immune genetic parameters in nonferrous metallurgy workers. The authors demonstrated differences in immune genetic profile between male and female workers, under exposure to combination of occupational hazards: noise and dust. Analysis of gene polymorphism revealed predominant disorders in the female workers, concerning criterion of prevalence for minor allele of genes in 1 and 2 phases of detoxication, neuro-endocrine regulation, lipid and energy metabolism, genes of immune regulation and apoptosis.
Assuntos
Poluentes Ocupacionais do Ar/efeitos adversos , Poeira/análise , Fenômenos do Sistema Imunitário/efeitos dos fármacos , Metalurgia , Ruído Ocupacional/efeitos adversos , Polimorfismo de Nucleotídeo Único/efeitos dos fármacos , Adulto , Poluentes Ocupacionais do Ar/análise , Estudos de Casos e Controles , Feminino , Genótipo , Humanos , Fenômenos do Sistema Imunitário/genética , Imunidade Celular/efeitos dos fármacos , Imunidade Celular/genética , Imunidade Humoral/efeitos dos fármacos , Imunidade Humoral/genética , Inativação Metabólica/efeitos dos fármacos , Inativação Metabólica/genética , Masculino , Polimorfismo de Nucleotídeo Único/imunologia , Federação Russa , Fatores SexuaisRESUMO
Medical examination covered female workers engaged into chemical production of technical rubber goods, under combined exposure to chemicals (nitrogen oxides, carbon oxide) and noise exceeding MAC by 3.7-27.5%. Findings are increased phagocytary activity, lower level of serum IgM and IgG, suppressed expression of CD25 and CD95 T-cell receptors, and slower apoptosis induced via Fas receptor--all those indicators associated with work conditions. The disorders revealed are actualized on a basis of genetic variability features of the females, that is characterized by prevalence of mutant allels of FAS and TNFR genes with minor homozygous phenotype.
