RESUMO
The rat aortic transplant model of chronic rejection was used to study the effect of cyclosporine (CsA) on the development of intimal hyperplasia. ACI and Lewis rat strains were used to create isograft and allograft CsA nontreated and treated groups. After orthotopic abdominal aortic transplantation, recipients received either no treatment, CsA 2.5 mg/kg/day, CsA 5 mg/kg/day, or CsA 10 mg/kg/day by gavage. Treated grafts were harvested at 3 and 6 months after transplantation, and computer image digital analysis was used to measure intimal and medial areas of graft cross-sections. At 3 months, the reduction in percent intima was 62% (P = 0.005), 74% (P = 0.002), and 97% (P < 0.0001) for the 2.5-, 5-, and 10-mg/kg allograft groups, respectively. There was a 93% (P < 0.0001) reduction in percent intima at 6 months in the 10-mg/kg allograft group. CsA treatment also preserved the aortic media. In comparison to nontreated isografts, medial area in nontreated allografts was decreased by 37% at 3 months after transplantation. In contrast, medial area was not significantly changed in CsA-treated recipients (10 mg/kg/day) in comparison to nontreated isografts. More importantly, medial nuclear density was preserved in the CsA-treated recipients in comparison to nontreated allografts and was similar to treated or nontreated isografts. In conclusion, daily high dose CsA treatment was found to markedly inhibit intimal hyperplasia in rat aortic allografts up to 6 months after transplantation, which suggests that CsA at an adequate dosage can suppress chronic rejection.
Assuntos
Aorta/transplante , Ciclosporina/farmacologia , Rejeição de Enxerto/prevenção & controle , Imunossupressores/farmacologia , Transplante Homólogo/imunologia , Transplante Isogênico/imunologia , Túnica Íntima/transplante , Túnica Média/transplante , Animais , Aorta/efeitos dos fármacos , Aorta/patologia , Ciclosporina/sangue , Rejeição de Enxerto/imunologia , Rejeição de Enxerto/patologia , Sobrevivência de Enxerto , Hiperplasia , Imunossupressores/sangue , Masculino , Ratos , Ratos Endogâmicos ACI , Ratos Endogâmicos Lew , Fatores de Tempo , Transplante Homólogo/patologia , Transplante Isogênico/patologia , Túnica Íntima/efeitos dos fármacos , Túnica Íntima/patologia , Túnica Média/efeitos dos fármacos , Túnica Média/patologiaAssuntos
Aorta Abdominal/transplante , Ciclosporina/uso terapêutico , Transplante Homólogo/imunologia , Transplante Isogênico/imunologia , Animais , Aorta Abdominal/efeitos dos fármacos , Aorta Abdominal/patologia , Rejeição de Enxerto/prevenção & controle , Hiperplasia , Músculo Liso Vascular/efeitos dos fármacos , Músculo Liso Vascular/patologia , Músculo Liso Vascular/transplante , Ratos , Ratos Endogâmicos ACI , Ratos Endogâmicos Lew , Transplante Homólogo/patologia , Transplante Isogênico/patologiaAssuntos
Ciclosporina/uso terapêutico , Sobrevivência de Enxerto/imunologia , Imunossupressores/uso terapêutico , Transplante de Fígado/imunologia , Ácido Micofenólico/análogos & derivados , Animais , Cães , Relação Dose-Resposta a Droga , Quimioterapia Combinada , Feminino , Sobrevivência de Enxerto/efeitos dos fármacos , Ácido Micofenólico/uso terapêutico , Prednisolona/uso terapêutico , Transplante HomólogoAssuntos
Aorta/transplante , Imunossupressores/farmacologia , Animais , Aorta/citologia , Aorta/efeitos dos fármacos , Compostos de Bifenilo/farmacologia , Divisão Celular/efeitos dos fármacos , Ciclosporina/farmacologia , Masculino , Músculo Liso Vascular/citologia , Músculo Liso Vascular/efeitos dos fármacos , Músculo Liso Vascular/transplante , Ácido Micofenólico/análogos & derivados , Ácido Micofenólico/farmacologia , Ratos , Ratos Endogâmicos ACI , Ratos Endogâmicos Lew , Transplante Homólogo , Transplante IsogênicoRESUMO
Recurrent squamous cell cancer of the vulva metastasized via regional lymphatics. Hematogenous spread is late and unusual. A patient with metastatic disease presented with soft tissue mass in her thigh musculature. We believe this to be the first reported case of noncontiguous skeletal metastasis for this tumor.
