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1.
AIDS Behav ; 2024 Jun 08.
Artigo em Inglês | MEDLINE | ID: mdl-38850332

RESUMO

The development of tailored interventions that address drug use and sexual risk taking among sexual minority men (SMM) in relationships has garnered increasing interest over the past 20 years. Motivational interviewing (MI) has demonstrated promise in both individual and couples-based applications. The Personal Values Card Sort activity is commonly employed in these interventions; however, relatively little is known about the content of client responses evoked by this intervention task. This study examined how SMM in relationships characterize their values; how their relationship partners influence value attainment; and how they situated drug use and sexual risk taking in the context of their values and primary relationships. A qualitative analysis of intervention transcripts from sessions with 47 SMM aged 18 to 34 was conducted. All respondents were HIV negative and indicated recent (past 30 days) drug use and sexual risk behavior. Participants' high priority values reflected a focus on clarifying personal identity and interpersonal relationships. Values manifested as realized, aspirational, or transformed. Participants described their partners as supporting goal attainment and as a companion with whom they cope with goal-related stressors. Consistent with interdependence theory, participants' responses indicated consideration of their partner and relationship when these aligned with and supported goal-attainment. Conversely, when partners were described as a barrier to realizing their values, participants characterized their relationship as being of lower quality. Implications of this content for the activation of motivation for health behavior are discussed with specific emphasis on applications with SMM in relationships.

3.
Women Health ; 63(9): 713-726, 2023 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-37794625

RESUMO

Research suggests that young adult women were among those more susceptible to mental health declines in the initial months of COVID-19. Unfortunately, longitudinal data examining mental health before and after the pandemic's onset are extremely limited. In a sample of 240 women ages 18-29 who were surveyed online first in November-December 2019 and then again between May and July 2020, this study aimed to examine how major life changes associated with the pandemic (i.e. loss of income, loss of employment, change in relationship status, and change in living arrangement) impacted mental health (i.e. depression, anxiety, stress). Multivariate regression analyses were conducted on three models predicting stress, anxiety, and depression from the four life changes, controlling for the effects of mental health before the COVID-19 pandemic. Results showed that a change in living arrangement was uniformly associated with increased mental health problems among women who also experienced a decrease in income. Likewise, loss of income was uniformly related to increased mental health problems among women who also experienced a change in living arrangement. In contrast, job loss was associated with a decrease in stress, and changes in relationship status were not associated with mental health outcomes. These findings highlight the potential for COVID-19 to produce co-occurring and synergistic stressors. Meanwhile, the impact of job loss on mental health may have been attenuated by enhanced unemployment benefits. Mental health interventions that aim to support young women as the pandemic abates should be tailored to address the impact of multiple psychosocial stressors.


Assuntos
COVID-19 , Saúde Mental , Adulto Jovem , Humanos , Feminino , COVID-19/epidemiologia , Depressão/epidemiologia , Depressão/psicologia , Pandemias , Saúde da Mulher , Ansiedade/epidemiologia , Ansiedade/psicologia
4.
Arch Sex Behav ; 52(7): 2859-2877, 2023 10.
Artigo em Inglês | MEDLINE | ID: mdl-37726556

RESUMO

Sexual awareness is an understudied phenomenon, despite its known effects on mental health. Little is known about the predictors of sexual awareness, including how early sexual debut and early engagement with online dating and hookup apps might impact the development of sexual mindfulness. Given the conceptual overlap between mindfulness and sexual awareness, this study tested a model that hypothesized that general mindfulness and early sexual and online dating debuts may be associated with mental health outcomes and sexual behavior through pathways involving sexual awareness (assertiveness, consciousness, appeal, and monitoring). A sample of 2,379 heterosexually active young adult women completed an online survey. Path models indicated that both early sexual debut (prior to age 15) and early online dating debut (prior to age 18) had significant, positive direct associations with anxiety and depression scores. Early online dating was also associated with condomless sex with casual male partners. However, both early sexual debut and early online dating debut were indirectly linked to greater sexual risk through greater appeal, and to lower sexual risk through sexual assertiveness. Additionally, greater monitoring contributed to more depression for those with an earlier sexual debut. These findings point to potential risks associated with early online dating. They also highlight benefits of studying sexual awareness as a multi-dimensional construct, especially as it helps to clarify divergent findings in the existing literature on the long-term consequences associated with early sexual debut. While some domains were associated with risk (monitoring and appeal), others were indicative of resilience (assertiveness).


Assuntos
Saúde Mental , Atenção Plena , Masculino , Humanos , Feminino , Adulto Jovem , Adolescente , Comportamento Sexual/psicologia , Parceiros Sexuais
5.
Anim Reprod Sci ; 252: 107227, 2023 May.
Artigo em Inglês | MEDLINE | ID: mdl-37027991

RESUMO

This experiment evaluated reproductive and productive responses of beef cows receiving self-fed low-moisture blocks (LMB) enriched or not with Ca salts of soybean oil (CSSO) throughout the breeding season. Non-pregnant, suckled multiparous Angus-influenced cows were assigned to a fixed-time artificial insemination (AI) protocol (day -10 to 0) followed by natural service (day 15-70). Cows were managed in 12 groups (46 ± 4 cows/group) maintained in individual pastures, and groups received LMB enriched with 25 % (as-fed basis) of CSSO or ground corn (CON) from day - 10 to 100. Both treatments were designed to yield a daily LMB intake of 0.454 kg/cow (as-fed basis). Cows receiving CSSO had greater (P < 0.01) mean concentrations of ω-6 fatty acids in plasma samples collected on days 0 and 55. Cows receiving CSSO had greater (P = 0.05) pregnancy rate to fixed-time AI (67.2 vs. 59.3 %), whereas final pregnancy rate did not differ (P = 0.92) between treatments. Pregnancy loss was less (P = 0.03) in CSSO cows (4.50 vs. 9.04 %), which also calved earlier during the calving season (treatment × week; P = 0.04). Weaning rate tended to be greater (P = 0.09) in CSSO (84.8 vs. 79.4 %), although calf weaning age and weight did not differ (P ≥ 0.72) between treatments. Kilos of calf weaned/cow exposed was greater (P = 0.04) in CSSO cows (234 vs. 215 kg). Therefore, supplementing CSSO to beef cows via LMB during the breeding season improved their reproductive and overall productivity during a cow-calf cycle.


Assuntos
Dieta , Suplementos Nutricionais , Gravidez , Feminino , Bovinos , Animais , Dieta/veterinária , Óleo de Soja/farmacologia , Sais , Melaço , Melhoramento Vegetal , Ração Animal/análise
6.
Sex Res Social Policy ; 19(2): 822-833, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-36212514

RESUMO

Introduction: Most sexual health interventions focus on individual-level predictors of sexual behavior. Given the considerable influence of environmental factors on adolescent girls' sexual health, current interventions may be insufficient to promote safer sex. In this study, we aimed to understand adolescent girls' anticipated barriers to engaging in safer sex behavior after completing a brief, web-based sexual health intervention called HEART. Methods: This study used qualitative interviews with 50 adolescent girls who were recruited from community-based organizations that serve vulnerable youth. All participants were 12 to 19 years old (mean age=15.62, SD=1.83), and identified with a marginalized racial/ethnic group (58% Black; 18% Latinx; 24% Asian, biracial, or multiracial). Further, 24% identified as LGBTQ+, and 58% were sexually active. Results: Guided by the social ecological model, we delineate six unique barriers to safer sex discussed by adolescents: partner manipulation, slut shaming, unclear sexual values, present time orientation, embarrassment, and access to sexual and reproductive health services. Discussion: We conclude with recommendations for addressing these barriers to optimize adolescent girls' sexual health.

7.
AIDS Behav ; 26(1): 13-20, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33929627

RESUMO

This study evaluated preliminary reliability and validity evidence for novel scores that extend the motivational interviewing treatment integrity (MITI) coding system to assess elements unique to motivational interviewing (MI) with couples. We recruited 20 cismale couples where at least one partner was aged 18-29; reported substance use; and was HIV-negative. Couples were randomized to a three-session MI or attention-matched education control. Four coders rated audio-recordings of initial sessions in both conditions. Novel global scores had high nearly-exact percent agreement (Decreasing Dyadic Ambivalence = 100%; Facilitating Dyadic Functioning = 94%). Consistency for novel behavior counts was good (ICCdyadic reflections = .72) and above minimum thresholds (nearly-exact agreement for Relationship Affirmations = 78%). MI sessions generated novel and established MITI codes that were significantly better than education sessions, providing preliminary evidence of validity. Findings provided initial support for the use of these novel scores in future research evaluating MI-based substance use and HIV risk reduction studies with couples.


Assuntos
Infecções por HIV , Entrevista Motivacional , Transtornos Relacionados ao Uso de Substâncias , Infecções por HIV/prevenção & controle , Humanos , Reprodutibilidade dos Testes , Transtornos Relacionados ao Uso de Substâncias/terapia
9.
J Adolesc Health ; 70(1): 28-41, 2022 01.
Artigo em Inglês | MEDLINE | ID: mdl-34059426

RESUMO

This meta-analysis synthesized the proportion of adolescents and young adults (AYAs) who are adherent to pre-exposure prophylaxis (PrEP) and factors moderating adherence. A systematic search was conducted of studies published through May 2020 using PubMed, PsycInfo, Cochrane, EMBASE, and CINAHL. Included studies reported the proportion of AYAs (mean age ≤ 29 years) who were PrEP adherent. PrEP adherence was meta-analyzed using random-effects models. Secondary analyses evaluated potential moderating variables. A total of 41 individual samples, from 29 studies, of 8,679 AYA (weighted average age = 23.8 years) were synthesized. The proportion of AYA who met study-defined thresholds for PrEP adherence was .64 (95% confidence interval .57-.71) across studies. Subgroup analyses revealed that adherence was lower in young cisgender women (.46) than young men who have sex with men (.65) and serodiscordant heterosexual couples (.98). Adherence was higher in studies initiated after 2012 (.70) than earlier studies (.47). No differences based on participant age, country, or strategies to promote adherence were found. Across published studies to date, 64% of AYA demonstrate adequate PrEP adherence. Findings can be used to guide PrEP adherence interventions and also provide directions for future research on PrEP among AYA.


Assuntos
Fármacos Anti-HIV , Infecções por HIV , Profilaxia Pré-Exposição , Minorias Sexuais e de Gênero , Adolescente , Adulto , Fármacos Anti-HIV/uso terapêutico , Feminino , Infecções por HIV/prevenção & controle , Homossexualidade Masculina , Humanos , Masculino , Adesão à Medicação , Adulto Jovem
11.
AIDS Behav ; 26(5): 1695-1715, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-34729670

RESUMO

HIV knowledge - the information a person possesses about HIV - is essential for the prevention and management of HIV. Therefore, the accurate measurement of HIV knowledge is important for both science and practice. This systematic review identifies extant HIV knowledge scales that have been validated with adolescent and adult populations and summarizes the state of this research. We searched seven electronic databases, which resulted in 6,525 articles. After title/abstract and full-text review, 27 studies remained and underwent qualitative review of reported scale psychometric properties. Many studies were conducted in the last decade (n = 12), reflecting advances in scientific knowledge of HIV. Five were exclusively adolescent-based studies (sample age ≤ 18). Most studies reported reliability (n = 25) or at least one form of validity (n = 21). Future studies should develop or refine HIV knowledge scales so that they reflect recent scientific developments, use rigorous psychometric testing, and target samples that include those persons at highest risk for HIV.


Assuntos
Infecções por HIV , Adolescente , Adulto , Infecções por HIV/diagnóstico , Infecções por HIV/prevenção & controle , Humanos , Psicometria , Reprodutibilidade dos Testes
12.
Psychol Sex Orientat Gend Divers ; 8(2): 201-212, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-34805445

RESUMO

Sexual minority men (SMM) disproportionately experience psychosocial risk factors and comorbid health concerns (e.g., HIV infection) that increase their vulnerability to COVID-19 infection and distress. The current study applied a socioecological approach to the Health Belief model to understand associations among perceived risk, optimistic bias (a perceived lower risk relative to similar others), united action (perceived community capacity for collective action) and COVID-19 behavioral prevention strategies among COVID-19-negative adult cisgender SMM. Participants (n = 859), recruited via a geo-location-based dating app in May 2020, completed an online survey. Results indicated significant interactions between perceived risk and optimistic bias on social distancing (B = -.03, p < .05), and between perceived risk and united action on number of preventive precautions (B = -.01, p < .01) and number of casual sex partners (B= -.20, p < .05). At low and average levels of perceived risk, socioecological constructs were positively associated with behavioral prevention strategies. At average levels of perceived risk and above, united action was negatively associated with number of casual sex partners, demonstrating evidence of the interaction between perception of interpersonal and community factors and perceptions of one's own risk. These findings may help to inform how to mobilize increased engagement in COVID-19 behavioral prevention strategies among cisgender SMM.

13.
Arch Sex Behav ; 50(4): 1505-1520, 2021 05.
Artigo em Inglês | MEDLINE | ID: mdl-34031778

RESUMO

Consensual non-monogamous (CNM) relationship rules, the boundaries and understandings partners have about acceptable behaviors related to their CNM relationship, are associated with relationship functioning and sexual health risk among gay and bisexual men. Partnered young gay and bisexual men (YGBM) experience unique relational challenges and sexual health disparities. Thus, understanding the nuances of relationship rules among YGBM in CNM relationships is of particular importance. However, few studies have examined relationship rules specifically among YGBM. The current study sought to explore relationship rules among YGBM in CNM relationships, including both those explicitly discussed and those implicitly assumed. We also assessed concordance and discrepancies in partners' reports of their relationship rules, as well as rule violations. Partners from 10 CNM couples (n = 20; Mage = 25.3) completed separate semi-structured interviews that included questions about their relationship rules. Individual- and dyad-level analyses were conducted using a modified version of the consensual qualitative research method. Three main themes of relationship rules emerged from the data: extradyadic partner criteria, conditions regarding extradyadic sex, and conditions regarding maintenance of the primary relationship. While 39% of participants reported rules that were not endorsed by their partner, few clearly contradictory discrepancies in partners' reports were observed. Nine participants reported having at least one implicit rule and 70% of participants (from 8 of 10 couples) reported that they and/or their partner violated at least one rule. Explicit rules and rule concordance may be associated with improved relationship functioning. Common violations of sexual risk reduction rules may contribute to YGBM's increased HIV/STI risk.


Assuntos
Infecções por HIV , Minorias Sexuais e de Gênero , Infecções Sexualmente Transmissíveis , Homossexualidade Masculina , Humanos , Masculino , Homens , Comportamento Sexual , Parceiros Sexuais
14.
J Sex Res ; 57(2): 145-154, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-31287336

RESUMO

This study evaluated the feasibility, acceptability, and preliminary efficacy of a 45-minute interactive, online sexual health program for adolescents, called Health Education and Relationship Training (HEART). The program was originally developed and evaluated among adolescent girls (HEART for Girls); the current project describes and evaluates a new version of the program that was adapted for boys and girls. Participants were 226 high school students (mean age = 16.3; 58% girls; 46% White; 79% heterosexual). Students were randomized to HEART or an attention-matched control and assessed at pre-test and immediate post-test. Overall, the program was feasible to administer in a school setting and youth found the program highly acceptable (83% liked the program, 87% learned new things, and 93% would use program content in the future). At post-test, students who completed HEART demonstrated improvements on every outcome we examined: sexual communication intentions, condom use intentions, HIV/STD knowledge, condom attitudes, condom norms, self-efficacy to practice safer sex, and sexual assertiveness compared to control participants (effect size ds = .23 to 1.27). Interactions by gender and sexual orientation revealed the program was equally acceptable and worked equally well for boys and girls and for heterosexual and sexual minority youth. We propose several avenues to further adapt and tailor HEART given its promise in promoting adolescent sexual health.


Assuntos
Comportamento do Adolescente , Comportamento Contraceptivo/estatística & dados numéricos , Educação Sexual/organização & administração , Saúde Sexual/estatística & dados numéricos , Infecções Sexualmente Transmissíveis/prevenção & controle , Adolescente , Estudos de Viabilidade , Feminino , Humanos , Internet , Masculino , Avaliação de Programas e Projetos de Saúde , Serviços de Saúde Escolar/organização & administração , Autoeficácia
15.
J Sex Res ; 57(2): 177-188, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-31517541

RESUMO

A healthy sexual self-concept that captures an understanding of the risky and the positive aspects of sexuality is imperative to life-long well-being. Parents have a unique opportunity to instill knowledge of sexual risk as well as confidence and comfort around sexuality in their adolescents. Although parent-child communication about sexual risk is fairly common, less is known regarding the frequency of parent-child communication about sex-positive topics, such as sexual desire and satisfaction. This study examined the frequency of parents' communication with their children about sexual risk and sex-positive topics among a sample of 901 parents of 13-17-year-old adolescents (parent Mage= 40.61; 71% mothers) from across the U.S. Parents reported on sexual communication with their adolescent children (child Mage = 14.68; 50% daughters). We examined gender differences in communication patterns. Few parents communicated with their adolescents about sex-positive topics. Only 38% discussed sexual satisfaction, 38% discussed different types of sexual practices (e.g., oral sex), and 55% discussed sexual desire. Parents communicate more about sexual risk than sex-positive topics with their adolescents and this discrepancy was largest for mothers of daughters. Fathers of daughters communicate the least about sex-positive topics. Implications for intervention development and future research on sexual communication are discussed.


Assuntos
Relações Pais-Filho , Educação Sexual/estatística & dados numéricos , Comportamento Sexual/estatística & dados numéricos , Saúde Sexual/estatística & dados numéricos , Adolescente , Estudos Transversais , Feminino , Humanos , Masculino , Caracteres Sexuais , Infecções Sexualmente Transmissíveis/prevenção & controle
16.
J Adolesc ; 77: 90-97, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31693971

RESUMO

INTRODUCTION: Adolescents are heterogeneous in how they define and experience their sexual orientation, which can include specific identity labels, romantic attractions, and other- and/or same-sex sexual behavior. These three components of sexual orientation are not always concordant, and studies suggest adolescents-particularly girls-are fluid in these dimensions of orientation over time. The current study examined: 1) fluidity in adolescent girls' and boys' self-labeled identities and romantic attractions over time, and 2) patterns of adolescent girls' and boys' self-labeled identities and romantic attractions as they coincide with sexual behavior. METHODS: Surveys were administered to adolescents in three low-income high schools in the rural Southeastern U.S. at three yearly intervals (n = 744; Mage = 15.0; 54.3% girls; 48% White, 24% Hispanic/Latinx, 21% Black/African American). Participants reported their self-labeled sexual identity and romantic attraction at each time point and their lifetime sexual behavior with girls and boys at year 3. RESULTS: Results revealed 26% of girls and 11% of boys reported fluidity in identity and 31% of girls and 10% of boys reported fluidity in attractions. At each time point, up to 20% of girls and 6% of boys reported a sexual minority identity label with concurrent same-sex attraction; the majority of these participants also reported same-sex behavior. Among heterosexual-identified participants reporting some degree of same-sex attraction at year 3, approximately 66% of girls and 10% of boys reported same-sex behavior. CONCLUSIONS: The findings suggest that many adolescents are nuanced and dynamic in how they identify and experience their developing sexualities.


Assuntos
Identidade de Gênero , Comportamento Sexual/psicologia , Adolescente , Feminino , Humanos , Masculino , Minorias Sexuais e de Gênero/psicologia , Minorias Sexuais e de Gênero/estatística & dados numéricos , Sudeste dos Estados Unidos , Inquéritos e Questionários
17.
J Health Commun ; 24(7-8): 633-642, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31407953

RESUMO

While the literature on parent-child sexual communication among adolescent girls is robust overall, research that is specifically focused on communication between fathers and daughters is more limited. Further, there have been calls for work on parent-child sexual communication to be situated within a multi-factorial conceptual framework that distinguishes between different communication components, such as the communication source, content, frequency, quality, and timing. Using such a framework, this study examined aspects of father-daughter sexual communication as they compare to mother-daughter communication in a diverse sample of 193 girls (Mage = 15.62). Results highlighted several gaps between father-daughter and mother-daughter communication. Girls reported covering less content and communicating less frequently about sexual topics with their fathers compared to their mothers. Girls also reported being less comfortable communicating and found their discussions to be less helpful with fathers than mothers. Girls were also less likely to report communicating with fathers about sexual topics before their sexual debut than with mothers. No significant differences were found in communication style (i.e., conversational or like a lecture) between fathers or mothers. Results highlight the importance of understanding the multifaceted process of parent-child communication and signal the need for targeted intervention efforts to improve upon father-daughter communication.


Assuntos
Comunicação , Relações Pai-Filho , Relações Mãe-Filho , Saúde Sexual , Adolescente , Feminino , Seguimentos , Humanos , Masculino , Inquéritos e Questionários
18.
AIDS Behav ; 23(8): 2155-2175, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30276703

RESUMO

Social support enhances self-management and prevention of behaviors and is typically assessed using self-report scales; however, little is known about the validity of these scales in HIV-infected or affected populations. This systematic review aims to identify available validated social support scales used in HIV-infected and HIV-affected populations. A systematic literature search using key search terms was conducted in electronic databases. After rounds abstract screenings, full-text reviews, and data abstraction 17 studies remained, two of which assessed multiple social support scales, which increased number of scales to 19. Most scales assessed positive social support behaviors (n = 18). Most scales assessed perceived social support (n = 14) compared to received social support. Reliability ranged from 0.67 to 0.97. The most common forms of validation reported were content validity and construct validity and the least was criterion-related validity. Future research should seek to build evidence for validation for existing scales used in HIV-infected or HIV-affected populations.


Assuntos
Infecções por HIV/psicologia , Apoio Social , HIV , Infecções por HIV/tratamento farmacológico , Humanos , Qualidade de Vida , Reprodutibilidade dos Testes , Autorrelato
19.
J Adolesc Health ; 62(6): 651-660, 2018 06.
Artigo em Inglês | MEDLINE | ID: mdl-29784112

RESUMO

PURPOSE: Technology-based interventions to promote sexual health have proliferated in recent years, yet their efficacy among youth has not been meta-analyzed. This study synthesizes the literature on technology-based sexual health interventions among youth. METHODS: Studies were included if they (1) sampled youth ages 13-24; (2) utilized technology-based platforms; (3) measured condom use or abstinence as outcomes; (4) evaluated program effects with experimental or quasi-experimental designs; and (5) were published in English. RESULTS: Sixteen studies with 11,525 youth were synthesized. There was a significant weighted mean effect of technology-based interventions on condom use (d = .23, 95% confidence interval [CI] [.12, .34], p < .001) and abstinence (d = .21, 95% CI [.02, .40], p = .027). Effects did not differ by age, gender, country, intervention dose, interactivity, or program tailoring. However, effects were stronger when assessed with short-term (1-5 months) than with longer term (greater than 6 months) follow-ups. Compared with control programs, technology-based interventions were also more effective in increasing sexual health knowledge (d = .40, p < .001) and safer sex norms (d = .15, p = .022) and attitudes (d = .12, p= .016). CONCLUSIONS: After 15 years of research on youth-focused technology-based interventions, this meta-analysis demonstrates their promise to improve safer sex behavior and cognitions. Future work should adapt interventions to extend their protective effects over time.


Assuntos
Comportamento Contraceptivo/estatística & dados numéricos , Gravidez não Planejada , Abstinência Sexual/estatística & dados numéricos , Infecções Sexualmente Transmissíveis/prevenção & controle , Adolescente , Adulto , Preservativos/estatística & dados numéricos , Comportamento Contraceptivo/psicologia , Feminino , Conhecimentos, Atitudes e Prática em Saúde , Humanos , Masculino , Gravidez , Ensaios Clínicos Controlados Aleatórios como Assunto , Sexo Seguro/psicologia , Abstinência Sexual/psicologia , Mídias Sociais , Adulto Jovem
20.
Theriogenology ; 117: 1-6, 2018 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-29800826

RESUMO

Urospermia is a common ejaculatory dysfunction of stallions. Current practice suggests that urine contaminated semen should not be used for cryopreservation. The aim of this study was to determine effects of urine contamination on semen freezing. Sixty-five ejaculates from eight stallions were divided into no urine (CONT), low (20% urine, LOW), and high (50% urine, HIGH) samples. Semen was extended with a commercial cooling extender, cushion-centrifuged, resuspended to 200 million/mL in a commercial egg-yolk based extender, and cryopreserved in liquid nitrogen. A subset of ejaculates (n = 20) were split in two after cushion-centrifugation, and one half of the ejaculate was submitted to a single-layer gradient centrifugation before cryopreservation. Sperm motility parameters were assessed pre- and post-freezing with an automated sperm analyzer. Semen pH, creatinine, and urea concentrations were assessed in raw samples, after urine contamination and after centrifugation and extension. Statistical analyses were performed with ANOVA and Tukey's posthoc. There were significant reductions in total and progressive sperm motilities (i.e., %TM and %PM, respectively) with increasing urine contamination pre-freezing (%TM 67 ±â€¯1.7, %PM 50 ±â€¯2.2, CONT), (%TM 60.3 ±â€¯1.7, % PM 42.5 ±â€¯2.1, LOW), and (%TM 41.3 ±â€¯2, %PM 21.3 ±â€¯1.5, HIGH). Post-thaw motilities for CONT (%TM 54 ±â€¯2.3, %PM 40.8 ±â€¯3.3) and LOW (%TM 51.7 ±â€¯1.8, %PM 36.2 ±â€¯2.1) were not different, but were higher than the HIGH (%TM 31.5 ±â€¯1.2, %PM 17.1 ±â€¯1.0) (p < 0.05). Post-thaw sperm viability was significantly lower in the HIGH (54.7 ±â€¯2.4) than in the CONT (63.8 ±â€¯2.3) or LOW (64.6 ±â€¯3.4) groups. Semen creatinine and urine levels were significantly higher with increasing urine contamination and were significantly decreased after centrifugation and resuspension in freezing extender. Pre-treatment semen pH was significantly lower than semen contaminated with low or high amounts of urine, and pH decreased significantly after centrifugation and resuspension. Gradient centrifugation did not improve %TM in the control group, but it did improve pre-freeze %TM and %PM in the low and high groups and improved significantly post freezing %TM and %PM in the high urine contaminated group. Semen contaminated with a small amount of urine may be suitable for freezing, whereas highly contaminated semen might not be usable. Although urine was mostly removed in this fashion, the initial exposure to high quantities was sufficient to decrease sperm motility pre- and post-freezing, whereas low urine contamination was not as detrimental.


Assuntos
Criopreservação/veterinária , Cavalos , Preservação do Sêmen/veterinária , Sêmen/química , Urina , Animais , Criopreservação/métodos , Masculino , Análise do Sêmen/veterinária , Preservação do Sêmen/métodos , Motilidade dos Espermatozoides
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