1.
FEBS Lett
; 199(2): 182-6, 1986 Apr 21.
Artigo
em Inglês
| MEDLINE
| ID: mdl-2422055
RESUMO
Amphiphilic detergent-soluble acetylcholinesterase (AChE) from Torpedo is converted to a hydrophilic form by digestion with phospholipase C from Trypanosoma brucei or from Bacillus cereus. This lipase digestion uncovers an immunological determinant which crossreacts with a complex carbohydrate structure present in the hydrophilic form of all variant surface glycoproteins (VSG) of T. brucei. This crossreacting determinant is also detected in human erythrocyte AChE after digestion with T. brucei lipase. From these results we conclude that the glycophospholipid anchors of protozoan VSG and of AChE of the two vertebrates share common structural features, suggesting that this novel type of membrane anchor has been conserved during evolution.
Assuntos
Acetilcolinesterase/imunologia , Antígenos de Protozoários/análise , Epitopos/análise , Membrana Eritrocítica/enzimologia , Glicoproteínas/imunologia , Trypanosoma brucei brucei/imunologia , Fosfolipases Tipo C , Acetilcolinesterase/metabolismo , Animais , Bacillus cereus/enzimologia , Membrana Celular/enzimologia , Órgão Elétrico/enzimologia , Glicosilfosfatidilinositol Diacilglicerol-Liase , Humanos , Cinética , Fosfolipases/metabolismo , Torpedo , Trypanosoma brucei brucei/enzimologia , Glicoproteínas Variantes de Superfície de Trypanosoma
2.
Prax Kinderpsychol Kinderpsychiatr
; 21(6): 221-38, 1972.
Artigo
em Alemão
| MEDLINE
| ID: mdl-5082279