How general are generalist parasites? The small mammal part of the Lyme disease transmission cycle in two ecosystems in northern Europe.

The pathogens causing Lyme disease are all vectored by generalist tick species found on a wide range of vertebrates, but spatial and annual variation in host use has rarely been quantified. We here compare the load of Ixodes ricinus (the vector) on small mammals and investigate the infection prevalence of Borrelia burgdorferi s.l. (the pathogen) involved in the enzootic transmission cycle of Lyme disease in two contrasting ecosystems in Norway from 2014 to 2016. The most common larval tick host in the eastern region was the bank vole, while the common shrew dominated in the western region of Norway. However, the wood mouse and the bank vole had consistently higher larval tick loads than the common shrew in both ecosystems. Hence, the evidence indicated that species are differently suitable as hosts, regardless of their abundances. The pathogen infection prevalence was similar among small mammal species, but markedly higher in the region with larger small mammal populations and higher tick loads, while the seasonal and annual variation was less marked. Our study indicated that the generalist I. ricinus shows consistent patterns of load on species of small vertebrate hosts, while B. burgdorferi s.l. (B. afzelii) was a true generalist. The similar roles of host species across regions suggest that disease dynamics can be predicted from host community composition, but predicting the role of host community composition for disease dynamics requires a detailed understanding of the different species population limitations under global change.

Tick abundance, pathogen prevalence, and disease incidence in two contrasting regions at the northern distribution range of Europe.

BACKGROUND: Emergence of tick-borne diseases is impacting humans and livestock across the Northern Hemisphere. There are, however, large regional variations in number of cases of tick-borne diseases. Some areas have surprisingly few cases of disease compared to other regions. The aim here is to provide a first step towards a better understanding of such contrasting regional patterns of disease emergences at the northern distribution range of Ixodes ricinus in Europe. METHODS: We compare disease incidence, vector abundance and pathogen prevalence in eastern and western Norway differing in the number of tick-borne disease cases. First, we analysed the incidence of Lyme borreliosis in humans, tick-borne fever (anaplasmosis) in sheep and anaplasmosis and babesiosis in cattle to verify if incidence differed. Secondly, we analysed extensive field data on questing tick density, pathogen prevalence, as well as the broad spatial pattern of human and livestock distribution as it may relate to tick exposure. RESULTS: The incidences of all diseases were lower in eastern, compared to western, Norway, but this was most marked for the livestock diseases. While the prevalence of Borrelia burgdorferi (sensu lato) in ticks was similar in the two regions, the prevalence of Anaplasma phagocytophilum was markedly lower in eastern, compared to western, Norway. We found overall a lower abundance of questing nymphs in the east. In the east, there were cases of babesiosis in cattle where anaplasmosis was absent, suggesting absence of the pathogen rather than differences in exposure to ticks as part of the explanation for the much lower incidence of anaplasmosis in eastern Norway. CONCLUSIONS: Many factors contribute to different disease incidence across ecosystems. We found that regional variation in tick-borne disease incidence may be partly linked to vector abundance and pathogen prevalence, but differently for human and livestock diseases. Further studies are needed to determine if there is also regional variation in specific genospecies and strain frequencies differing in pathogenicity.

Contrasting emergence of Lyme disease across ecosystems.

Global environmental changes are causing Lyme disease to emerge in Europe. The life cycle of Ixodes ricinus, the tick vector of Lyme disease, involves an ontogenetic niche shift, from the larval and nymphal stages utilizing a wide range of hosts, picking up the pathogens causing Lyme disease from small vertebrates, to the adult stage depending on larger (non-transmission) hosts, typically deer. Because of this complexity the role of different host species for emergence of Lyme disease remains controversial. Here, by analysing long-term data on incidence in humans over a broad geographical scale in Norway, we show that both high spatial and temporal deer population density increase Lyme disease incidence. However, the trajectories of deer population sizes play an overall limited role for the recent emergence of the disease. Our study suggests that managing deer populations will have some effect on disease incidence, but that Lyme disease may nevertheless increase as multiple drivers are involved.